The role of sphingosine kinases on the formation of neural network in dentate granule cells of hippocampus

• Project/Area Number: 21590309
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kobe University
• Principal Investigator: OKADA Taro 神戸大学, 大学院・医学研究科, 准教授 (80304088)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: スフィンゴシンキナーゼ / スフィンゴ脂質 / スフィンゴシン1-リン酸 / 神経伝達物質放出

Research Abstract

It was shown that the long-term potentiation(LTP) which is know to be involved in the memory and learning is not observed in sphingosine kinase 1(SPHK1)-knockout mice. As a consequence the spatial learning ability of the knock out mice was impaired. We also showed that SPHK1 knock down by siRNA diminished the synapse formation in primary hippocampal cells. These results are very important in the sense that the function of hippocampus might be regulated by the medicines which act on the SPHK1-S1P axis. Since over-activation of hippocampal neuron is considered to be involved in the onset of some kind of epilepsy, our finding may provide new treatment strategy of the epilepsy.

Research Products

• [Journal Article] Regulation of synaptic strength by sphingosine 1-phosphate in the hippocampus (2010)
  • Author(s): Kanno T, Nishizaki T, Proia RL, Kajimoto T, Jahangeer S, Okada T, Nakamura S
  • Journal Title: Neuroscience Volume: 29 Pages: 973-80
• [Journal Article] Sphingosine kinase 1 regulates mucin production via ERK phosphorylation (2010)
  • Author(s): Kono Y, Nishiuma T, Okada T, Kobayashi K, Funada Y, Kotani Y, Jahangeer S, Nakamura S, Nishimura Y
  • Journal Title: Pulm Pharmacol Ther Volume: 23 Pages: 36-42
  • NAID: 120003290046
• [Journal Article] Roles of extracellular and intracellular sphingosine 1-phosphate in cell migration (2009)
  • Author(s): Yu H, Okada T, Kobayashi M, Abo-Elmatty DM, Jahangeer S, Nakamura S
  • Journal Title: Genes Cells Volume: 14 Pages: 597-605
• [Journal Article] CtBP1/BARS is an activator of phospholipase D1 necessary for agonist-induced macropinocytosis (2009)
  • Author(s): Haga Y, Miwa N, Jahangeer S, Okada T, Nakamura S
  • Journal Title: EMBO J Volume: 28 Pages: 1197-207
• [Journal Article] Sphingosine kinase/sphingosine 1-phosphate signalling in central nervous system (2009)
  • Author(s): Okada T, Kajimoto T, Jahangeer S, Nakamura S
  • Journal Title: Cell Signal Volume: 21 Pages: 7-13
• [Presentation] Sphingosine 1-phosphate in physiology and pathology in the brain (2009)
  • Author(s): Nakamura, S. and Okada, T.
  • Organizer: 9th International Congress of the Polish Neuroscience Society in Warsow
  • Place of Presentation: Poland
• [Book] 細胞内外で機能するスフィンゴシン1リン酸(S1P)の役割 (2012)
  • Author(s): 岡田太郎、中村俊一
  • Publisher: 生化学
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/biochemistry/Home.html
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/biochemistry/Home.html
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/biochemistry/publication.html
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/biochemistry/publication.html