Host-defense by excretion and it's failure generating diseases

• Project/Area Number: 21590323
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Nagasaki International University
• Principal Investigator: WADA Morimasa 長崎国際大学, 薬学部 (20220965)
• Co-Investigator(Kenkyū-buntansha): FUJITA Kyoko 長崎国際大学, 薬学部, 助手 (50435137)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 膜輸送 / 輸送タンパク質 / 生体防御 / 分子間相互作用 / 腸管腫瘍形成 / トランスポーター / 発がん / 薬物治療抵抗性 / 生体分子 / タンパク質 / 分子医薬学 / 分子相互作用

Research Abstract

Excretion at the tissue and organs bordering on external side of the body has a role as a host-defense function against xenobiotics and, then, it's failure could cause diseases. Identification of the corresponding molecular factors and elucidation of the underlying molecular mechanisms is indispensable for diagnosis and treatment of the diseases. In this study, (1) we identified some of the molecular factors which could be a target for cancer treatment, and (2) found that inhibitor of the xenobiotics transporter could be useful as a chemoprevention agent.

Research Products

• [Journal Article] Suppression of intestinal polyp development in ApcMin/+mice through inhibition of P-glycoprotein using verapamil (2012)
  • Author(s): Kyoko Fujimoto, Gen Fujii, Michihiro Mutoh, Masa, Yasunaga, Hiromitsu Tanaka, Morimasa Wada
  • Journal Title: European Journal of Cancer Prevention Volume: (in press)
  • Peer Reviewed
• [Journal Article] Suppression of intestinal polyp development in ApcMin/+ mice through inhibition of P-glycoprotein using verapamil (2012)
  • Author(s): Kyoko Fujimoto, Gen Fujii, Michihiro Mutoh, Masa Yasunaga, Hiromitsu Tanaka, Morimasa Wada
  • Journal Title: European Journal of Cancer Prevention Volume: (in press) Issue: 1 Pages: 8-10
  • DOI: 10.1097/cej.0b013e328353ede8
  • Peer Reviewed
• [Journal Article] Gene knockout and metabolome analysis of carnitine/organic cation transporter OCTN1 (2010)
  • Author(s): Kato Y, Kubo Y, Iwata D, Kato S, Sudo T, Sugiura T, Kagaya T, Wakayama T, Hirayama A, Sugimoto M, Sugihara K, Kaneko S, Soga T, Asano M, Tomita M, Matsui T, Wada M, Tsuji A.
  • Journal Title: Pharm Res Volume: 27 Pages: 832-840
  • NAID: 120002224385
  • Peer Reviewed
• [Journal Article] Gene knockout and metabolome analysis of carnitine/organic cation transporter OCTN1. (2010)
  • Author(s): Kato Y, Kubo Y, Iwata D, Kato S, Sudo T, Sugiura T, Kagaya T, Wakayama T, Hirayama A, Sugimoto M, Sugihara K, Kaneko S, Soga T, Asano M, Tomita M, Matsui T, Wada M, Tsuji A.
  • Journal Title: Pharm Res Volume: 27 Pages: 832-840
  • NAID: 120002224385
  • Peer Reviewed
• [Journal Article] OAZ-t/OAZ3 is essential for rigid connection of sperm tails to heads in mouse (2009)
  • Author(s): Tokuhiro K, Isotani A, Yokota S, Yano Y, Oshio S, Hirose M, Wada M, Fujita K, Ogawa Y, Okabe M, Nishimune Y, Tanaka H.
  • Journal Title: PLoS, Genet Volume: 5 Pages: 1000712-1000712
  • Peer Reviewed
• [Journal Article] Unique expression of encoding an acrosomal protein(ACPIN1) and salivary-specific protein(SAGSIN1) alternatively translated from two open reading frames on Acpin1 mRNA (2009)
  • Author(s): Ueda T, Manabe H, Keizo T, Hirose M, Matsuoka Y, Miyagawa Y, Tsujimura A, Fujita K, Wada M, Okuyama A, Tanaka H.
  • Journal Title: Int. J. Urol Volume: 16 Pages: 639-646
  • Peer Reviewed
• [Journal Article] OAZ-t/OAZ3 is essential for rigid connection of sperm tails to heads in mouse. (2009)
  • Author(s): Tokuhiro K, Isotani A, Yokota S, Yano Y, Oshio S, Hirose M, Wada M, Fujita K, Ogawa Y, Okabe M, Nishimune Y, Tanaka H.
  • Journal Title: PLoS Genet. 5
  • Peer Reviewed
• [Journal Article] Unique expression of encoding an acrosomal protein (ACPIN1) and salivary-specific protein (SAGSIN1) alternatively translated from two open reading frames on Acpinl mRNA. (2009)
  • Author(s): Ueda T, Manabe H, Keizo T, Hirose M, Matsuoka Y, Miyagawa Y, Tsujimura A, Fujita K, Wada M, Okuyama A, Tanaka H.
  • Journal Title: Int.J.Urol. 16 Pages: 639-646
  • Peer Reviewed
• [Journal Article] Gene knockout and metabolome analysis of carnitine/organic cation transporter OCTN1.
  • Author(s): Kato Y, Kubo Y, Iwata D, Kato S, Sudo T, Sugiura T, Kagaya T, Wakayama T, Hirayama A, Sugimoto M, Sugihara K, Kaneko S, Soga T, Asano M, Tomita M, Matsui T, Wada M, Tsuji A.
  • Journal Title: Pharm Res (印刷中)
  • NAID: 120002224385
  • Peer Reviewed
• [Presentation] 多剤耐性タンパク質の細胞内局在化におけるリシン・リッチ領域の役割 (2011)
  • Author(s): 南恵美、藤本京子、榎田陽介、横田貞記、和田守正
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] 多剤耐性タンパク質の細胞内局在化におけるリシン・リッチ領域の役割 (2011)
  • Author(s): 南恵美、藤本京子、榎田陽介、横田貞記、和田守正
  • Organizer: 第34回・日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] ApcMin/+マウスにおける腫瘍サイズ依存的遺伝子の同定と機能解析 (2010)
  • Author(s): 藤本京子、仲西定祐、田口健一、田中宏光、安田香央里、田代康介、久原哲、和田守正
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Apc^<Min/+>マウスにおける腫瘍サイズ依存的遺伝子の同定と機能解析 (2010)
  • Author(s): 藤本京子、仲西定祐、田口健一、田中宏光、安田香央里、田代康介、久原哲、和田守正
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Apc Min/+マウスにおける腫瘍サイズ別遺伝子発現パターンの解析 (2009)
  • Author(s): 藤田京子、田口健一、安田香央里、田中宏光、田代康介、久原哲、和田守正
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Book] コンパスシリーズ「分子生物学-創薬・テーラーメード医療に向けて-」 (2010)
  • Author(s): 和田守正
  • Publisher: 南江堂
• [Book] コンパスシリーズ「分子生物学-創薬・テーラーメード医療に向けて-」 (2010)
  • Author(s): 和田守正(共著)
  • Total Pages: 238
  • Publisher: 南江堂
• [Remarks]