Elucidation of the function of Aurora kinases in mitosis and itsdysregulation in cancer

• Project/Area Number: 21590331
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Gifu University
• Principal Investigator: KIMURA Masashi 岐阜大学, 医学系研究科, 助教 (40260575)
• Co-Investigator(Kenkyū-buntansha): OKANO Yukio 岐阜大学, 医学系研究科, 教授 (10177066)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: がん / オーロラキナーゼ / 細胞分裂 / 細胞死 / 転写制御

Research Abstract

Our study using Aurora inhibitor indicate that Aurora is essentialfor cell division and survival. Several proteins, which are regulated by Aurora kinases,play important roles in mitosis. RNA interference of centrosomal proteins includingAurora A induced cell death, which depended on the checkpoint proteins. The expressionof Aurora A and Plk1 were upregulated in cancer cells, but those of the enzymes whichdegrade them were downregulated.

Research Products

• [Journal Article] Mitotic catastrophe and cell deathinduced by depletion of centrosomalproteins (2013)
  • Author(s): Kimura, M., Yoshioka, T., Saio, M.,Banno, Y., Nagaoka, H., and Okano, Y
  • Journal Title: Cell Death Dis Volume: 4 Issue: 4 Pages: e603-e603
  • DOI: 10.1038/cddis.2013.108
  • Peer Reviewed
• [Journal Article] Identification of a Rho family specificguanine nucleotide exchange factorFLJ00018, as a novel actin-bindingprotein (2013)
  • Author(s): Sato, K., Handa, H., Kimura, M., Okano,Y., Nagaoka, H., Nagase, T., Sugiyama,T., Kitade, Y., and Ueda, H.
  • Journal Title: Cell. Signal. Volume: 25 Issue: 1 Pages: 41-49
  • DOI: 10.1016/j.cellsig.2012.09.015
  • Peer Reviewed
• [Journal Article] Growth suppression and mitotic defect inducedby JNJ-7706621, an inhibitor ofcyclin-dependent kinases and aurorakinases (2012)
  • Author(s): Matsuhashi, A., Ohno, T., Kimura, M.,Hara, A., Saio, M., Nagano, A., Kawai,G., Saitou, M., Takigami, I., Yamada, K.,Okano, Y., and Shimizu, K
  • Journal Title: Curr. Cancer. Drug. Targets. Volume: 12 Pages: 625-639
  • URL: http://www.eurekaselect.com/98883/article
  • Peer Reviewed
• [Journal Article] Growth suppression and mitotic defect induced by JNJ-7706621, an inhibitor of cyclin-dependent kinases and aurora kinases. (2012)
  • Author(s): Matsuhashi A
  • Journal Title: Current Cancer Drug Targets Volume: 12 Pages: 625-639
  • Peer Reviewed
• [Journal Article] Growth Suppression and Mitotic Defect Induced by JNJ-7706621, an Inhibitor of Cyclin-Dependent Kinases and Aurora Kinases (2012)
  • Author(s): Matsuhashi Aya
  • Journal Title: Curr Cancer Drug Targets Volume: (In press)
  • Peer Reviewed
• [Journal Article] Plk1 is negativelyregulated by RNF8. Biochem (2011)
  • Author(s): Yoshioka, T., Kimura, M., Saio, M., Era,S., and Okano, Y
  • Journal Title: Biophys.Res. Commun. Volume: 410 Issue: 1 Pages: 57-6
  • DOI: 10.1016/j.bbrc.2011.05.104
  • Peer Reviewed
• [Journal Article] Effects ofAZD1152, a selective Aurora B kinaseinhibitor, on Burkitt's and Hodgkin's lymphomas (2011)
  • Author(s): Mori, N., Ishikawa, C., Senba, M.,Kimura, M., and Okano, Y.
  • Journal Title: Biochem. Pharmacol. Volume: 81 Issue: 9 Pages: 1106-1115
  • DOI: 10.1016/j.bcp.2011.02.010
  • Peer Reviewed
• [Journal Article] Plk1 is negatively regulated by RNF8 (2011)
  • Author(s): Yoshioka Takashi
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 57-61
  • Peer Reviewed
• [Journal Article] Effects of AZD1152, a selective Aurora B kinase inhibitor, on Burkitt's and Hodgkin's lymphomas (2011)
  • Author(s): Mori Naoki
  • Journal Title: Biochemical Pharmacology Volume: 81 Pages: 1106-1115
  • Peer Reviewed
• [Journal Article] Aberrantpromoter hypermethylation of the CHFRgene in oral squamous cell carcinomas. (2009)
  • Author(s): Baba, S., Hara, A., Kato, K., Long, N.K.,Hatano, Y., Kimura, M., Okano, Y.,Yamada, Y., and Shibata, T
  • Journal Title: Oncol. Rep. Volume: 22 Pages: 1173-1179
  • URL: http://www.spandidos-publications.com/or/22/5/1173
  • Peer Reviewed
• [Journal Article] Aberrant promoter hypermethylation of the CHFR gene in oral squamous cell carcinomas. (2009)
  • Author(s): Baba Seiji
  • Journal Title: Oncology Reports 22 Pages: 1173-1179
  • Peer Reviewed