• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanisms for switching off of CaM-kinases and their involvement in regulation of the cellular phosphorylation balance

Research Project

Project/Area Number 21590334
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionHiroshima University

Principal Investigator

ATSUHIKO Ishida  広島大学, 大学院・総合科学研究科, 准教授 (90212886)

Co-Investigator(Kenkyū-buntansha) TAKESHI Yamazaki  広島大学, 大学院・総合科学研究科, 教授 (30192397)
TATSUO Nehira  広島大学, 大学院・総合科学研究科, 助教 (60321692)
Co-Investigator(Renkei-kenkyūsha) TAKAO Mukuda  広島大学, 大学院・総合科学研究科, 助教 (60346335)
ISAMU Kameshita  香川大学, 農学部, 教授 (60127941)
NORIYUKI Sueyoshi  香川大学, 農学部, 准教授 (90346635)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords分子病態学 / CaMKP-N / PPM1E / プロテインホスファターゼ / CaMキナーゼ / 無細胞タンパク合成 / プロセシング / 活性断片 / 酵素学的解析 / プロテインキナーゼ / プロテアソーム / 細胞内局在 / 基質ターゲッティング / 活性制御 / インゲルホスファターゼアッセイ / 酵素 / シグナル伝達 / カルシウム情報伝達系 / カルモジュリン / 発生異常 / 脳神経疾患
Research Abstract

To clarify intracellular dynamics and physiological significance of CaM-kinase phosphatse(CaMKP/ PPM1F) and its nuclear homolog, CaMKP-N(PPM1E), which had been identified as protein phosphatases involved in negative regulation of multifunctional CaM-kinases, we carried out functional analyses of these enzymes using zebrafish as a model system. These in vitro and in vivo studies revealed that CaMKP plays a pivotal role in embryogenesis of zebrafish, and that processing of CaMKP-N regulates its catalytic activity and subcellular localization.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (33 results)

All 2012 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (18 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Functional processing of nuclearCa^<2+>/ calmodulin-dependent protein kinasephosphatase(CaMKP-N): evidence for acritical role of proteolytic processing inthe regulation of its catalytic activitysubcellular localization and substratetargeting in vivo2012

    • Author(s)
      Sueyoshi N., NimuraT., OnouchiT., BabaH., TakenakaS., Ishida, A. Kameshita, I.
    • Journal Title

      Arch. Biochem. Biophys

      Volume: 517 Pages: 43-52

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional processing of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N) : evidence for a critical role of proteolytic processing in the regulation of its catalytic activity, subcellular localization and substrate targeting in vivo2012

    • Author(s)
      Sueyoshi, N., et al
    • Journal Title

      Arch.Biochem.Biophys.

      Volume: 517 Issue: 1 Pages: 43-52

    • DOI

      10.1016/j.abb.2011.10.017

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synaptic vesicles are capable of synthesizing the VGLUT substrate glutamate from α-ketoglutarate for vesicular loading2012

    • Author(s)
      Takeda K, et al
    • Journal Title

      J.Neurochem.

      Volume: (未定)(印刷中) Issue: 2 Pages: 184-196

    • DOI

      10.1111/j.1471-4159.2012.07684.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In-Gel Protein Phosphatase Assays and Other Useful Methods for the Detection of Protein Phosphatase Activities2011

    • Author(s)
      Ishida, A., Kameshita, I
    • Journal Title

      Anticancer Agents Med. Chem

      Volume: 11 Pages: 47-53

    • URL

      http://www.benthamdirect.org/pages/content.php?ACAMC/2011/00000011/00000001/0005W.SGM

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] In-Gel Protein Phosphatase Assays and Other Useful Methods for the Detection of Protein Phosphatase Activities2011

    • Author(s)
      A.Ishida, et al.
    • Journal Title

      Anti-Cancer Agents in Medicinal Chemistry

      Volume: 11 Pages: 47-53

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CoPK32 is a novel stress-responsive protein kinase in the mushroom Coprinopsis cinerea2011

    • Author(s)
      K.Kaneko, et al.
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: (印刷中 未定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A minimum size homologue of Ca^<2+>/ calmodulin-dependent protein kinaseIV naturally occurring in zebrafish2010

    • Author(s)
      Nimura, T., Sugiyama, Y., Sueyoshi, N., Shigeri, Y., Ishida, A., Kameshita, I.
    • Journal Title

      J. Biochem

      Volume: 147 Issue: 6 Pages: 857-865

    • DOI

      10.1093/jb/mvq021

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oxazolone-induced over-expression of focal adhesion kinase in colonic epithelial cells of colitis mouse model2010

    • Author(s)
      Yanju Ma, et al.
    • Journal Title

      FEBS Letters

      Volume: 584 Pages: 3949-3954

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A minimum size homologue of Ca^<2+>/calmodulin-dependent protein kinase IV naturally occurring in zebrafish2010

    • Author(s)
      Nimura, T., et al.
    • Journal Title

      J.Biochem. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kameshita Ica^<2+>/ calmodulin-dependent protein kinase phosphatase(CaMKP) is indispensable fornormal embryogenesis in zebrafish, Daniorerio2009

    • Author(s)
      Sueyoshi N., Nimura T., Ishida A., Taniguchi T., Yoshimura Y., Ito M., Shigeri Y.
    • Journal Title

      Arch. Biochem. Biophys

      Volume: 488 Issue: 1 Pages: 48-59

    • DOI

      10.1016/j.abb.2009.06.003

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ca^<2+>/calmodulin-dependent protein kinase phosphatase (CaMKP) is indispensable for normal embryogenesis in zebrafish, Dabio rerio2009

    • Author(s)
      Sueyoshi, N., et al.
    • Journal Title

      Arch.Biochem.Biophys. 488

      Pages: 48-59

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Retinoic acid stimulates 17β-estradiol and teststerone synthesis in rat hippocampal slice cultures2009

    • Author(s)
      Munetsuna, E., et al.
    • Journal Title

      Endocrinology 150

      Pages: 4260-4269

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] ゼブラフィッシュで探るCa2+/カルモジュリン依存性プロテインキナーゼホスファターゼ(CaMKP/ PPM1F)と核局在型CaMKP(CaMKP. N/ PPM1E)の機能と活性調節機構2012

    • Author(s)
      末吉紀行1, 石田敦彦2, 亀下勇
    • Organizer
      1香川大学農学部応用生物科学科、2広島大学大学院総合科学研究科第84回日本生化学会大会
    • Place of Presentation
      京都市・京都国際会館(招待講演)
    • Year and Date
      2012-09-23
    • Related Report
      2011 Final Research Report
  • [Presentation] in vitro合成系で調製したヒトCaMキナーゼホスファターゼ-N(PPM1E)の酵素学的性質2012

    • Author(s)
      大上恵1、宮本和寛1、竹中康浩2、茂里康2、五島直樹2、末吉紀行3、亀下勇3、山崎岳1、石田敦彦1
    • Organizer
      1広島大学大学院総合科学研究科、2産業技術総合研究所、3香川大学農学部応用生物科学科第52回日本生化学会中国・四国支部総会
    • Place of Presentation
      広島市・広島大学霞キャンパス
    • Year and Date
      2012-05-14
    • Related Report
      2011 Final Research Report
  • [Presentation] Functional Processing of NuclearCa2+/ Calmodulin-dependent Protein KinasePhosphatase(CaMKP-N) International Symposium on CarcinogenicSpiral & 9th International2012

    • Author(s)
      Sueyoshi N., NimuraT., OnouchiT., Baba H., Takenaka S., Ishida A., KameshitaI.
    • Organizer
      Conference onProtein Phosphatase
    • Place of Presentation
      東京都・東京大学
    • Year and Date
      2012-02-01
    • Related Report
      2011 Final Research Report
  • [Presentation] ゼブラフィッシュで探るCa^<2+>/カルモジュリン依存性プロテインキナーゼホスファターゼ(CaMKP/PPM1F)と核局在型CaMKP (CaMKP-N/PPM1E)の機能と活性調節機構2011

    • Author(s)
      末吉紀行, ら
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都市(招待講演)
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] 担子菌キノコにおける新規ストレス応答性プロテインキナーゼの機能解析2011

    • Author(s)
      金子啓祐, ら
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都市
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 異なる飼育環境におけるトリメチルスズの中枢神経毒性評価2011

    • Author(s)
      大山勤, ら
    • Organizer
      第34回日本神経科学会大会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-09-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 核局在型Ca2+/カルモジュリン依存性プロテインキナーゼホスファターゼ(CaMKP-N・PPM1E)の機能的な限定分解2011

    • Author(s)
      小野内貴士1、二村貴樹1、馬場裕美1、竹中忍1、石田敦彦2、亀下勇1、末吉紀行1
    • Organizer
      香川大学農学部応用生物科学科、2広島大学大学院総合科学研究科第52回日本生化学会中国・四国支部総会
    • Place of Presentation
      広島市・広島大学霞キャンパス
    • Year and Date
      2011-05-14
    • Related Report
      2011 Final Research Report
  • [Presentation] in vitro合成系で調製したヒトCaMキナーゼホスフアターゼーN(PPMIE)の酵素学的性質2011

    • Author(s)
      大上恵, ら
    • Organizer
      第52回日本生化学会中国・四国支部総会
    • Place of Presentation
      広島市
    • Year and Date
      2011-05-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functional Processing of Nuclear Ca2+/Calmodulin-dependent Protein Kinase Phosphatase(CaMKP-N)2011

    • Author(s)
      Sueyoshi N, et al.
    • Organizer
      International Symposium on Carcinogenic Spiral & 9th International Conference on Protein Phosphatase
    • Place of Presentation
      東京都
    • Year and Date
      2011-02-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] 蛍光検出円二色性(FDCD)を用いたタンパク質立体構造のピンポイント解析の試み2010

    • Author(s)
      石原郁, ら
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 核局在型Ca2+/カルモジュリン依存性プロテインキナーゼホスファターゼ(CaMKP-N)の機能的な限定分解2010

    • Author(s)
      小野内貴士1、二村貴樹1、馬場裕美1、竹中忍1、石田敦彦2、亀下勇1、末吉紀行1
    • Organizer
      1香川大・農・応用生物科学、2広島大院・総合科学第83回日本生化学会大会
    • Place of Presentation
      神戸市・神戸ポートアイランド
    • Year and Date
      2010-12-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 核局在型Ca^<2+>/カルモジュリン依存性プロテインキナーゼホスファターゼ(CaMKP-N)の機能的な限定分解2010

    • Author(s)
      小野内貴士, ら
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] αケトグルタル酸はシナプス小胞におけるグルタミン酸の重要な前駆体である2009

    • Author(s)
      武田晃治, ら
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] α-Ketoglutarate as a precursor of the neurotransmitter glutamate2009

    • Author(s)
      武田晃治, et al.
    • Organizer
      2009 Neuroscience Meeting
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2009-10-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] Social isolation stimulates hippocampal estradiol synthesis2009

    • Author(s)
      M.HATTORI, et al.
    • Organizer
      2009 Neuroscience Meeting
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2009-10-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] Retinoic acid stimulates estradiol and testosterone synthesis in rat hippocampal slice cultures2009

    • Author(s)
      T.YAMAZAKI, et al.
    • Organizer
      2009 Neuroscience Meeting
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2009-10-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] CaMキナーゼ特異的フォスファターゼ2009

    • Author(s)
      石田敦彦
    • Organizer
      ナナカマド研究会
    • Place of Presentation
      旭川市・旭川医科大学
    • Year and Date
      2009-09-10
    • Related Report
      2011 Final Research Report
  • [Presentation] CaMキナーゼ特異的フォスファターゼ2009

    • Author(s)
      石田敦彦
    • Organizer
      旭川医科大学生化学講座 ナナカマド研究会
    • Place of Presentation
      旭川医科大学
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Book] 基礎の有機化学.生命科学のために2010

    • Author(s)
      深宮齋彦、本田計一、石田敦彦、大村尚、根平達夫共著
    • Total Pages
      232
    • Publisher
      三共出版
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/ishiyasu/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/ishiyasu/

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi