Functional analysis on Goliath, a novel regulator of Wnt signaling

• Project/Area Number: 21590336
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Oita University
• Principal Investigator: HAMADA Fumihiko 大分大学, 医学部, 教授 (70252707)
• Co-Investigator(Kenkyū-buntansha): JI Ruichen 大分大学, 医学部, 助教 (60305034)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 分子腫瘍学 / 細胞内シグナル伝達 / 発生学 / ショウジョウバエ / ショウジョバエ

Research Abstract

We have performed functional analysis on Goliath(Gol), a novel regulator of Wnt signaling. We have identified an E2 ubiquitin conjugating enzyme which binds to the RING domain of Gol. In addition, by searching for the PA domain-binding proteins, some putative substrates of Gol have also been found. To elucidate in vivo function of Gol, we have generated a Drosophila line in which the gol gene was deleted.

Research Products

• [Journal Article] Sunspot, a link between Wingless signaling and endoreplication in Drosophila (2010)
  • Author(s): Taniue, K., Nishida, A., Hamada, F., Sugie, A., Oda, T., Ui-Tei, K., Tabata, T., Akiyama, T.
  • Journal Title: Development Volume: 137 Pages: 1755-1764
  • Peer Reviewed
• [Journal Article] Multiple expressions of lymphatic markers and morphological evolution of newly formed lymphatics in lymphangioma and lymph node lymphangiogenesis (2010)
  • Author(s): Ji, RC., Eshita, Y., Xing, L., Miura, M.
  • Journal Title: Microvasc Res Volume: 80 Pages: 195-195
  • Peer Reviewed
• [Journal Article] Morphological maturation level of the esophagus is associated with the number of circumesophageal muscle fibers during archenteron formation in the starfish Patiria(Asterina) pectinifera (2010)
  • Author(s): Miguchi, Y., Takata, H., Doihara, T., Miyawaki, K., Shimokawa, T., Hamada, F., Kobayashi, N., Matsuda, S.
  • Journal Title: Biol. Bull Volume: 219 Pages: 12-12
  • Peer Reviewed
• [Journal Article] Morphological maturation level of the esophagus is associated with the number of circumesophageal muscle fibers during archenteron formation in the starfish Patiria (Asterina) pectinifera (2010)
  • Author(s): Miguchi, Y., Takata, H., Doihara, T., Miyawaki, K., Shimokawa, T., Hamada, F., Kobayashi, N., Matsuda, S.
  • Journal Title: Biol.Bull. Volume: 219 Pages: 12-16
  • Peer Reviewed
• [Journal Article] Sunspot, a link between Wingless signaling and endoreplication in Drosophila (2010)
  • Author(s): Taniue, K.
  • Journal Title: Development (in press)
  • Peer Reviewed
• [Journal Article] Spatiotemporal distribution patterns of oligosaccharides during early embryogenesis in the starfish Patiria pectinifera (2009)
  • Author(s): Doihara, T., Miguchi, Y., Miyawaki, K., Shimokawa, T., Hamada, F., Kobayashi, N., Matsuda, S.
  • Journal Title: Dev. Genes Evol Volume: 219 Pages: 199-206
  • Peer Reviewed
• [Journal Article] Spatiotemporal distribution patterns of oligosaccharides during early embryogenesis in the starfish Patiria pectinifera (2009)
  • Author(s): Doihara, T.
  • Journal Title: Dev.Genes Evol 219 Pages: 199-206
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Matsuda, S.
  • Journal Title: Dendritic Spines : Biochemistry, Modeling and Properties(NOVA Science publisher) Pages: 151-162
• [Presentation] Wntシグナル伝達経路を制御する新規ubiquitin ligaseの機能解析 (2009)
  • Author(s): 濱田文彦
  • Organizer: 日本解剖学会第65回九州支部学術集会
  • Place of Presentation: 琉球大学・医学部
  • Year and Date: 2009-11-07
• [Presentation] Wnt シグナル伝達経路を制御する新規 ubiquitin ligase の機能解析 (2009)
  • Author(s): 濱田文彦
  • Organizer: 日本解剖学会 第65回九州支部学術集会
  • Place of Presentation: 琉球大学医学部
  • Year and Date: 2009-11-07
• [Book] The Structure of Dendritic Spines, Dendritic Spines : Biochemistry, Modeling and Properties (2009)
  • Author(s): Matsuda, S., Hamada, F., Ishizuka, N.
  • Publisher: NOVA Science publisher