Research on early diagnosis of abnormal carbohydrate metabolism by antibody specific for AGEs
| Project/Area Number |
21590340
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Japan Women's University |
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Principal Investigator |
NAGAI Ryoji 日本女子大学, 家政学部, 講師 (20315295)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | AGEs / メイラード反応 / 糖化 / CML / コラーゲン / 老化 / 翻訳後修飾 / 糖尿病合併症 / フマル酸 |
|---|
| Research Abstract |
Pentosidine, one of advanced glycation end-products(AGEs), is measured by HPLC in the serum, and increased by heating in a temperature-and time-dependent manner. The increase in pentosidine formation is significantly inhibited by acidic conditions. This indicates that the level of serum pentosidine will be measured more accurately by ELISA if hydrochloric acid is added during the heating process. Furthermore, N^ω-carboxymethylarginine(CMA), acid labile AGE structure, is preferentially generated in glycated collagen. We elucidated the hot-spot of CMA formation in glycated collagen.
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Report
(4 results)
Research Products
(83 results)
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[Journal Article] Natural Compounds Containing a Catechol Group Enhance the Formation of N^ε-(carboxymymethyl) lysine of the Maillard Reaction2011
Author(s)
Fujiwara Y, Kiyota N, Tsurushima K, Yoshitomi M, Mera K, Sakashita N, Takeya M, Ikeda T, Araki T, Nohara T, Nagai R.
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Journal Title
Free Radic. Biol. Med
Volume: 50(7)
Pages: 883-891
Related Report
Peer Reviewed
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[Journal Article] Involvement of membrane type-1-matrix metalloproteinase(MT1-MMP) in RAGE activation signaling pathways2011
Author(s)
Kamioka M, Ishibashi T, Ohkawara H, Nagai R, Sugimoto K, Uekita H, Matsui T, Yamagishi S, Ando K, Sakamoto T, Sakamoto N, Takuwa Y, Wada I, Shimoi M, Maruyama Y, Takeishi Y.
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Journal Title
J. Cell. Physiol
Volume: 226(6)
Pages: 1554-1563
Related Report
Peer Reviewed
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[Journal Article] Involvement of membrane type1-matrix metalloproteinase(MT1-MMP) in rage activation signalingpathways2011
Author(s)
Kamioka M, Ishibashi T, Ohkawara H, Eguchi R, Sugimoto K, Uekita H, Matsui T, Yamagishi SI, Ando K, Sakamoto T, Sakamoto N, Takuwa Y, Wada I, Shiomi M, Maruyama Y, Takeishi Y.
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Journal Title
J Cell Physiol
Volume: 226(6)
Issue: 6
Pages: 1554-1563
DOI
Related Report
Peer Reviewed
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[Journal Article] Glycation of a food allergen by the Maillard reaction enhances its T-cell immunogenicity : the role of macrophage scavenger receptor class A type I and II2010
Author(s)
Ilchmann A, Burgdorf S, Scheurer S, Waibler Z, Nagai R, Wellner A, Yamamoto Y, Yamamoto H, Henle T, Kurts C, Kalinke U, Vieths S, Toda M
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Journal Title
J Allergy Clin Immunol
Volume: 125(1)
Pages: 175-183
Related Report
Peer Reviewed
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[Journal Article] Angptl 4 Deficiency Improves Lipid Metabolism, Suppresses Foam Cell Formation and Protects Against Atherosclerosis2009
Author(s)
Adachi H, Fujiwara Y, Kondo T, Nishikawa T, Ogawa R, Matsumura T, Ishii N, Nagai R, Miyata K, Tabata M, Motoshima H, Furukawa N, Tsuruzoe K, Kawashima J, Takeya M, Yamashita S, Koh GY, Suda T, Oike Y, Araki E
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 379(4)
Pages: 806-811
Related Report
Peer Reviewed
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