Role of Wnt-5a signaling in tumors

• Project/Area Number: 21590341
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Kagoshima University
• Principal Investigator: KISHIDA Shosei 鹿児島大学, 医歯学総合研究科, 教授 (50274064)
• Co-Investigator(Kenkyū-buntansha): KISHIDA Michiko 鹿児島大学, 大学院・医歯学総合研究科, 助教 (40274089)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: Wnt / シグナル伝達 / 脳腫瘍 / グリオーマ / 口腔上皮 / 不死化 / エナメル上皮種 / MMP-2 / 細胞運動 / 下垂体細腫瘍 / エナメル上皮腫

Research Abstract

We analyzed the expressions of all Wnts and Frizzleds in several human glioma cell lines and found that Wnt-5A, Wnt-7A, Frizzled-2, and Frizzled-6 were highly expressed in those cells. By Wnt-5A-knockdown experiment, we have shown that Wnt-5A stimulated induced cellular motility and invasion of human glioma cells through the induction of MMP-2 mRNA expression and MMP-2 enzymatic activity. We established normal human epithelial cells without using HPV and SV40 genes, which have the potential to induce carcinogenesis.

Research Products

• [Journal Article] Subcellular localization and putative r ole of VPS13A/chorein in dopaminergic neuronal cells (2012)
  • Author(s): Hayashi T., Kishida M., Nishizawa Y., Iijima M., Koriyama C., Nakamura M., Sano A., Kishida S.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 419巻 Pages: 511-516
  • Peer Reviewed
• [Journal Article] Subcellular localization and putative role of VPS13A/chorein in dopaminergic neuronal cells (2012)
  • Author(s): Hayashi T., et al
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 419巻 Issue: 3 Pages: 511-516
  • DOI: 10.1016/j.bbrc.2012.02.047
  • Peer Reviewed
• [Journal Article] Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes (2011)
  • Author(s): Kibe T., Kishida M., Kamino M., Iijima M., Chen L., Habu M., Miyawaki A., Hijioka H., Nakamura N., Kiyono T., Kishida S.
  • Journal Title: Oral Science International Volume: 8巻 Pages: 20-28
  • NAID: 10030270663
  • Peer Reviewed
• [Journal Article] Wnt-5a signaling is correlated with infiltrative activity in human glioma by inducing celluar migration and MMP-2 (2011)
  • Author(s): Kamino M., Kishida M., Kibw T., Iijima M., Hirono H., Tokudome M., Chen L., Koriyama C., Yamada K., Arita K., Kishida S.
  • Journal Title: Cancer Science Volume: 102巻3号 Pages: 540-548
  • Peer Reviewed
• [Journal Article] Immortalization and characterization of normal oral epithelial cells with out using HPV and SV40 genes (2011)
  • Author(s): Kibe T., et al
  • Journal Title: Oral Science International Volume: 8巻 Pages: 20-28
  • Peer Reviewed
• [Journal Article] Wnt-5a signaling is correlated with infiltrative activity in human glioma by inducing cellular migration and MMP-2. (2011)
  • Author(s): Kamino M., et al.
  • Journal Title: Cancer Science Volume: 3巻 Pages: 540-548
  • Peer Reviewed
• [Journal Article] Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes. (2011)
  • Author(s): Kibe T., et al.
  • Journal Title: Oral Science International Volume: (in press)
  • Peer Reviewed
• [Presentation] The role of Wnt signaling pathway in growth characteristics of Ameloblastoma cells (2011)
  • Author(s): 岐部俊郎, 岸田想子, 比地岡浩志, 宮脇昭彦, 飯島幹雄, 神野真幸, 清野透, 中村典史, 岸田昭世
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜(神奈川県)
  • Year and Date: 2011-12-14
• [Presentation] The role of Wnt signaling pathway in growth characteristics of Ameloblastoma cells (2011)
  • Author(s): 岐部俊郎, 他
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-14
• [Presentation] Glioma浸潤活性におけるWntシグナルの作用機構 (2011)
  • Author(s): 羽生未佳, 神野真幸, 岸田想子, 岐部俊郎, 湯之上俊二, 飯島幹雄, 平野宏文, 岸田昭世, 時村洋, 有田和徳
  • Organizer: 第29回日本脳腫瘍学会
  • Place of Presentation: 下呂(岐阜県)
  • Year and Date: 2011-11-27
• [Presentation] GliomaにおけるWntシグナル分子の作用機構 (2011)
  • Author(s): 羽生未佳, 神野真幸, 岸田想子, 湯之上俊二, 飯島幹雄, 平野宏文, 岸田昭世, 有田和徳
  • Organizer: 第12回日本分子脳神経外科学会
  • Place of Presentation: 横浜(神奈川県)
  • Year and Date: 2011-11-15
• [Presentation] GliomaにおけるWntシグナル分子の作用機構 (2011)
  • Author(s): 羽生未佳, 他
  • Organizer: 日本分子脳神経外科学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-11-14
• [Presentation] The role of Wnt signaling pathway in gr owth characterisitics of Ameloblastome cells (2011)
  • Author(s): 岐部俊郎, 岸田昭世, 中村典史
  • Organizer: インドネシア口腔外科学会
  • Place of Presentation: インドネシア
  • Year and Date: 2011-09-22
• [Presentation] The role of Wnt signaling pathway in growth characterisitics of Ameloblastome cells (2011)
  • Author(s): Kibe T., et al
  • Organizer: インドネシア口腔外科学会
  • Place of Presentation: インドネシア
  • Year and Date: 2011-09-20
• [Presentation] 新規口腔粘膜上皮細胞株MOE1の樹立～HPVやSV40遺伝子を用いない不死化 (2011)
  • Author(s): 岐部俊郎, 比地岡浩志, 宮脇昭彦, 中村典史, 岸田昭世
  • Organizer: 第65回日本口腔科学学会
  • Place of Presentation: 船堀(東京都)
  • Year and Date: 2011-04-21
• [Presentation] Wnt-5a signaling is correlated with infltrative activity in human glioma by inducing cellular migration and MMP-2 (2010)
  • Author(s): 神野真幸, 岸田想子, 岐部俊郎, 生駒今日子, 飯島幹雄, 平野宏文, 徳留舞, 陳琳, 郡山千早, 武田泰生, 山田勝士, 有田和徳, 岸田昭世
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] Wnt-5a signahng is correlated with cellular motility and MMP-2 in human glioma cells. (2010)
  • Author(s): Kamino M., et al.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-08
• [Presentation] ヒトグリオーマ浸潤活性におけるWntシグナルの作用機構 (2010)
  • Author(s): 神野真幸, 岸田想子, 岐部俊郎, 生駒今日子, 飯島幹雄, 平野宏文, 徳留舞, 陳琳, 郡山千早, 武田泰生, 山田勝士, 有田和徳, 岸田昭世
  • Organizer: 第63回日本薬理学会西南部会
  • Place of Presentation: 鹿児島(鹿児島県)
  • Year and Date: 2010-11-26
• [Presentation] エナメル上皮腫でのWntシグナルの作用解析及び、新規正常口腔粘膜上皮細胞株の樹立 (2010)
  • Author(s): 岐部俊郎, 比地岡浩志, 宮脇昭彦, 光安岳志, 岸田想子, 清野透, 岸田昭世, 中村典史
  • Organizer: 第55回日本口腔外科学会
  • Place of Presentation: 幕張(千葉県)
  • Year and Date: 2010-10-16
• [Presentation] ヒトグリオーマにおけるWntシグナルの作用解析(細胞運動を中心とする) (2010)
  • Author(s): 神野真幸, 岐部俊郎, 中村葉子, 生駒今日子, 武田泰生, 山田勝士, 岸田想子, 飯島幹雄, 平野宏文, 岸田昭世
  • Organizer: 平成22年度日本生化学会九州支部例会
  • Place of Presentation: 鹿児島(鹿児島県)
  • Year and Date: 2010-05-23
• [Presentation] GliomaにおけるWntシグナル分子の発現解析 (2009)
  • Author(s): 神野真幸, 岐部俊郎, 中村葉子, 生駒今日子, 武田泰生, 山田勝士, 平野宏文, 岸田想子, 飯島幹雄, 岸田昭世
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜(神奈川県)
  • Year and Date: 2009-12-10
• [Presentation] Glioma における Wnt シグナル分子の発現解析 (2009)
  • Author(s): 神野正幸, ほか
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜