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Role of Wnt-5a signaling in tumors

Research Project

Project/Area Number 21590341
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKagoshima University

Principal Investigator

KISHIDA Shosei  鹿児島大学, 医歯学総合研究科, 教授 (50274064)

Co-Investigator(Kenkyū-buntansha) KISHIDA Michiko  鹿児島大学, 大学院・医歯学総合研究科, 助教 (40274089)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsWnt / シグナル伝達 / 脳腫瘍 / グリオーマ / 口腔上皮 / 不死化 / エナメル上皮種 / MMP-2 / 細胞運動 / 下垂体細腫瘍 / エナメル上皮腫
Research Abstract

We analyzed the expressions of all Wnts and Frizzleds in several human glioma cell lines and found that Wnt-5A, Wnt-7A, Frizzled-2, and Frizzled-6 were highly expressed in those cells. By Wnt-5A-knockdown experiment, we have shown that Wnt-5A stimulated induced cellular motility and invasion of human glioma cells through the induction of MMP-2 mRNA expression and MMP-2 enzymatic activity. We established normal human epithelial cells without using HPV and SV40 genes, which have the potential to induce carcinogenesis.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010 2009

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (15 results)

  • [Journal Article] Subcellular localization and putative r ole of VPS13A/chorein in dopaminergic neuronal cells2012

    • Author(s)
      Hayashi T., Kishida M., Nishizawa Y., Iijima M., Koriyama C., Nakamura M., Sano A., Kishida S.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 419巻 Pages: 511-516

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Subcellular localization and putative role of VPS13A/chorein in dopaminergic neuronal cells2012

    • Author(s)
      Hayashi T., et al
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 419巻 Issue: 3 Pages: 511-516

    • DOI

      10.1016/j.bbrc.2012.02.047

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes2011

    • Author(s)
      Kibe T., Kishida M., Kamino M., Iijima M., Chen L., Habu M., Miyawaki A., Hijioka H., Nakamura N., Kiyono T., Kishida S.
    • Journal Title

      Oral Science International

      Volume: 8巻 Pages: 20-28

    • NAID

      10030270663

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Wnt-5a signaling is correlated with infiltrative activity in human glioma by inducing celluar migration and MMP-22011

    • Author(s)
      Kamino M., Kishida M., Kibw T., Iijima M., Hirono H., Tokudome M., Chen L., Koriyama C., Yamada K., Arita K., Kishida S.
    • Journal Title

      Cancer Science

      Volume: 102巻3号 Pages: 540-548

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immortalization and characterization of normal oral epithelial cells with out using HPV and SV40 genes2011

    • Author(s)
      Kibe T., et al
    • Journal Title

      Oral Science International

      Volume: 8巻 Pages: 20-28

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wnt-5a signaling is correlated with infiltrative activity in human glioma by inducing cellular migration and MMP-2.2011

    • Author(s)
      Kamino M., et al.
    • Journal Title

      Cancer Science

      Volume: 3巻 Pages: 540-548

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes.2011

    • Author(s)
      Kibe T., et al.
    • Journal Title

      Oral Science International

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] The role of Wnt signaling pathway in growth characteristics of Ameloblastoma cells2011

    • Author(s)
      岐部俊郎, 岸田想子, 比地岡浩志, 宮脇昭彦, 飯島幹雄, 神野真幸, 清野透, 中村典史, 岸田昭世
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜(神奈川県)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Final Research Report
  • [Presentation] The role of Wnt signaling pathway in growth characteristics of Ameloblastoma cells2011

    • Author(s)
      岐部俊郎, 他
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Glioma浸潤活性におけるWntシグナルの作用機構2011

    • Author(s)
      羽生未佳, 神野真幸, 岸田想子, 岐部俊郎, 湯之上俊二, 飯島幹雄, 平野宏文, 岸田昭世, 時村洋, 有田和徳
    • Organizer
      第29回日本脳腫瘍学会
    • Place of Presentation
      下呂(岐阜県)
    • Year and Date
      2011-11-27
    • Related Report
      2011 Final Research Report
  • [Presentation] GliomaにおけるWntシグナル分子の作用機構2011

    • Author(s)
      羽生未佳, 神野真幸, 岸田想子, 湯之上俊二, 飯島幹雄, 平野宏文, 岸田昭世, 有田和徳
    • Organizer
      第12回日本分子脳神経外科学会
    • Place of Presentation
      横浜(神奈川県)
    • Year and Date
      2011-11-15
    • Related Report
      2011 Final Research Report
  • [Presentation] GliomaにおけるWntシグナル分子の作用機構2011

    • Author(s)
      羽生未佳, 他
    • Organizer
      日本分子脳神経外科学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-11-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] The role of Wnt signaling pathway in gr owth characterisitics of Ameloblastome cells2011

    • Author(s)
      岐部俊郎, 岸田昭世, 中村典史
    • Organizer
      インドネシア口腔外科学会
    • Place of Presentation
      インドネシア
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] The role of Wnt signaling pathway in growth characterisitics of Ameloblastome cells2011

    • Author(s)
      Kibe T., et al
    • Organizer
      インドネシア口腔外科学会
    • Place of Presentation
      インドネシア
    • Year and Date
      2011-09-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新規口腔粘膜上皮細胞株MOE1の樹立~HPVやSV40遺伝子を用いない不死化2011

    • Author(s)
      岐部俊郎, 比地岡浩志, 宮脇昭彦, 中村典史, 岸田昭世
    • Organizer
      第65回日本口腔科学学会
    • Place of Presentation
      船堀(東京都)
    • Year and Date
      2011-04-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Wnt-5a signaling is correlated with infltrative activity in human glioma by inducing cellular migration and MMP-22010

    • Author(s)
      神野真幸, 岸田想子, 岐部俊郎, 生駒今日子, 飯島幹雄, 平野宏文, 徳留舞, 陳琳, 郡山千早, 武田泰生, 山田勝士, 有田和徳, 岸田昭世
    • Organizer
      第33回日本分子生物学会
    • Place of Presentation
      神戸(兵庫県)
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Wnt-5a signahng is correlated with cellular motility and MMP-2 in human glioma cells.2010

    • Author(s)
      Kamino M., et al.
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒトグリオーマ浸潤活性におけるWntシグナルの作用機構2010

    • Author(s)
      神野真幸, 岸田想子, 岐部俊郎, 生駒今日子, 飯島幹雄, 平野宏文, 徳留舞, 陳琳, 郡山千早, 武田泰生, 山田勝士, 有田和徳, 岸田昭世
    • Organizer
      第63回日本薬理学会西南部会
    • Place of Presentation
      鹿児島(鹿児島県)
    • Year and Date
      2010-11-26
    • Related Report
      2011 Final Research Report
  • [Presentation] エナメル上皮腫でのWntシグナルの作用解析及び、新規正常口腔粘膜上皮細胞株の樹立2010

    • Author(s)
      岐部俊郎, 比地岡浩志, 宮脇昭彦, 光安岳志, 岸田想子, 清野透, 岸田昭世, 中村典史
    • Organizer
      第55回日本口腔外科学会
    • Place of Presentation
      幕張(千葉県)
    • Year and Date
      2010-10-16
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒトグリオーマにおけるWntシグナルの作用解析(細胞運動を中心とする)2010

    • Author(s)
      神野真幸, 岐部俊郎, 中村葉子, 生駒今日子, 武田泰生, 山田勝士, 岸田想子, 飯島幹雄, 平野宏文, 岸田昭世
    • Organizer
      平成22年度日本生化学会九州支部例会
    • Place of Presentation
      鹿児島(鹿児島県)
    • Year and Date
      2010-05-23
    • Related Report
      2011 Final Research Report
  • [Presentation] GliomaにおけるWntシグナル分子の発現解析2009

    • Author(s)
      神野真幸, 岐部俊郎, 中村葉子, 生駒今日子, 武田泰生, 山田勝士, 平野宏文, 岸田想子, 飯島幹雄, 岸田昭世
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜(神奈川県)
    • Year and Date
      2009-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Glioma における Wnt シグナル分子の発現解析2009

    • Author(s)
      神野正幸, ほか
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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