| Project/Area Number |
21590341
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
KISHIDA Shosei 鹿児島大学, 医歯学総合研究科, 教授 (50274064)
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| Co-Investigator(Kenkyū-buntansha) |
KISHIDA Michiko 鹿児島大学, 大学院・医歯学総合研究科, 助教 (40274089)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | Wnt / シグナル伝達 / 脳腫瘍 / グリオーマ / 口腔上皮 / 不死化 / エナメル上皮種 / MMP-2 / 細胞運動 / 下垂体細腫瘍 / エナメル上皮腫 |
|---|
| Research Abstract |
We analyzed the expressions of all Wnts and Frizzleds in several human glioma cell lines and found that Wnt-5A, Wnt-7A, Frizzled-2, and Frizzled-6 were highly expressed in those cells. By Wnt-5A-knockdown experiment, we have shown that Wnt-5A stimulated induced cellular motility and invasion of human glioma cells through the induction of MMP-2 mRNA expression and MMP-2 enzymatic activity. We established normal human epithelial cells without using HPV and SV40 genes, which have the potential to induce carcinogenesis.
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