Functional analysis of Fanconi anemia gene

• Project/Area Number: 21590345
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: MATSUSHITA Nobuko 東京薬科大学, 生命科学部, 准教授 (30333222)
• Co-Investigator(Kenkyū-buntansha): YANAGI Shigeru 東京薬科大学, 生命科学部, 教授 (60252003)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 分子病態学 / DNA損傷修復 / 染色体 / ファンコニ貧血 / チェックポイント

Research Abstract

We performed a functional analysis of Fanconi anemia gene FANCD2.Previously studies reported that FANCD2 functions in DNA repair. However, we found that FANCD2 also involves in transcriptional regulation of TNF-α gene through binding to its promoter region, which resulted in enhanced TNF-αmRNA level in FANCD2 deficient cells.

Research Products

• [Journal Article] Direct Inhibition of TNF-α Promoter Activity by Fanconi Anemia Prote in FANCD2 (2011)
  • Author(s): Nobuko Matsushita, Yujiro Endo, Koichi Sato, Hitoshi Kurumizaka, Takayuki Yamashita, Minoru Takata, Shigeru Yanagi
  • Journal Title: PLoS One Volume: 6(電子媒体による発表) Issue: 8 Pages: e23324-e23324
  • DOI: 10.1371/journal.pone.0023324
  • Peer Reviewed
• [Journal Article] Distinct regulation of mitochondrial localization and stability of two human Sirt5 isoforms (2011)
  • Author(s): Matsushita, N., Yonashiro, R., Ogata, Y., Sugiura, A., Nagashima, S., Fukuda, T., Inatome, R., and Yanagi, S
  • Journal Title: Genes Cells Volume: 16(2) Pages: 190-202
  • Peer Reviewed
• [Journal Article] A mitochondrial ubiquitin ligase MITOL controls cell toxicity of polyglutamine-expanded protein (2011)
  • Author(s): Sugiura, A., Yonashiro, R., Fukuda, T., Matsushita, N., Nagashima, S., Inatome, R., and Yanagi, S
  • Journal Title: Mitochondrion Volume: 11(1) Issue: 1 Pages: 139-146
  • DOI: 10.1016/j.mito.2010.09.001
  • Peer Reviewed
• [Journal Article] Distinct regulation of mitochondrial localization and stability of two human Sirt5 isoforms (2011)
  • Author(s): Matsushita, N.
  • Journal Title: Genes Cells Volume: 16 Pages: 190-202
  • Peer Reviewed
• [Journal Article] A mitochondrial ubiquitin ligase MITOL controls cell toxicity of polyglutamine-expanded protein (2011)
  • Author(s): Sugiura, A.
  • Journal Title: Mitochondrion Volume: 11 Pages: 139-146
  • Peer Reviewed
• [Journal Article] Mitochondrial ubiquitin ligase MITOL ubiquitinates mutant SOD1 and attenuates mutant SOD1-induced ROS generation (2009)
  • Author(s): Yonashiro, R., Sugiura, A., Miyachi, M., Fukuda, T., Matsushita, N., Inatome, R., Ogata, Y., Suzuki, T., Dohmae, N., and Yanagi, S
  • Journal Title: Mol. Biol. Cell Volume: 20(21) Issue: 21 Pages: 4524-4530
  • DOI: 10.1091/mbc.e09-02-0112
  • Peer Reviewed
• [Journal Article] Mitochondrial ubiquitin ligase MITOL ubiquitinates mutant SOD1 and attenuates mutant SOD1-induced ROS generation. (2009)
  • Author(s): Yonashiro, R.
  • Journal Title: Mol.Biol.Cell 20 Pages: 4524-4530
  • Peer Reviewed
• [Presentation] ファンコニ貧血蛋白FANCD2によるNF-kappaB転写制御機構の解析FANCD2 regulates NF-kappaB transcriptional activity (2011)
  • Author(s): 松下暢子, 菊間啓太, 太田莉英子, 尊龍介, 徳山剛士, 高田穣, 胡桃坂仁志, 山下孝之, 稲留涼子, 柳茂
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] Analysis of the Fanconi Anemia pathway (2011)
  • Author(s): 菊間啓太, 松下暢子, 志村卓哉, 川口浩平, 吉田有里, 加藤翔太, 稲留涼子, 柳茂
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] ファンコニ貧血蛋白FANCD2によるNF-kappaB転写制御機構の解析 (2011)
  • Author(s): 松下暢子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシィフィコ横浜(横浜)
  • Year and Date: 2011-12-15
• [Presentation] Analysis of the Fanconi Anemia pathway (2011)
  • Author(s): 菊間啓太
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシィフィコ横浜(横浜)
  • Year and Date: 2011-12-15
• [Presentation] TAX1BP1欠損DT40細胞の機能解析～NF-κB転写活性亢進が細胞に与える影響の検討～Functional Analysis of TAX1BP1 deficient cells (2011)
  • Author(s): 太田莉英子, 松下暢子, 尊龍介, 徳山剛士, 稲留涼子, 柳茂
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] TAX1BP1欠損DT40細胞の機能解析～NF-κB転写活性亢進が細胞に与える影響の検討～ (2011)
  • Author(s): 太田莉英子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシィフィコ横浜(横浜)
  • Year and Date: 2011-12-14
• [Presentation] ファンコニ貧血経路(FA経路)の解析Analysis of the Fanconi anemia pathway (2010)
  • Author(s): 遠藤雄二郎、松下暢子、菊間啓太、太田莉英子、稲留涼子、柳茂
  • Organizer: BMB 2010第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] ファンコニ貧血経路(FA経路)の解析 (2010)
  • Author(s): 遠藤雄二郎
  • Organizer: BMB2010第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] ミトコンドリアユビキチンリガーゼMITOLによるミトコンドリアダイナミクスの制御 (2009)
  • Author(s): 君嶋悠矢、杉浦歩、長谷川江里香、渡辺香林、遠藤雄二郎、松下暢子、與那城亮、柳茂
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] ミトコンドリアユビキチンリガーゼMITOLによるミトコンドリアダイナミクスの制御 (2009)
  • Author(s): 君嶋悠矢
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-10