| Project/Area Number |
21590349
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Chiba Cancer Center (Research Institute) |
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Principal Investigator |
KOSHIKAWA Nobuko 千葉県がんセンター(研究所), 研究局, 研究員 (90260249)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKENAGA Keizo 島根大学, 医学部・生命科学講座腫瘍生物学, 准教授 (80260256)
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| Research Collaborator |
HAYASHI Jun-ichi 筑波大学, 生命環境科学研究科(系), 教授 (60142113)
OHIRA Miki 千葉県がんセンター(研究所), 研究局, 研究員 (20311384)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | mtDNA変異 / 肺癌 / 大腸癌 / 原発巣 / 転移巣 / 神経膠腫 / 髄液播種 / ラマンバンド / ROS / 転移能 / 肺癌・大腸癌 / 癌部と非癌部 / 神経膠芽腫 / mtDNA / 変異 / ヒト腫瘍 |
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| Research Abstract |
We showed that ROS-generating pathogenic mutations in NADH dehydrogenase subunit 6(ND6) gene of mitochondrial DNA(mtDNA), which is a subunit of complex I of the electron transport chain, can regulate tumor cell metastasis in mouse models. In this study, we compared the status of mutations and nonsynonymous single nucleotide polymorphisms(SNPs) of the genes encoding complex I subunits(ND1, ND2, ND3, ND4L and ND6) between primary and metastatic lesions of human lung and colon carcinomas. The combined frequency of appearance of nonsynonymous SNPs and mutations related to mtDNA base substitution disease such as LHON and novel mutations of Grantham value> 50 in the genes encoding complex I was considerably higher in metastatic lesions than in primary lesions. It may be possible that such mtDNA SNPs and mutations contribute to metastasis in human cancers.
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