Plk1-mediated signaling pathway in cancer cells

• Project/Area Number: 21590352
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: GOTO Hidemasa 愛知県がんセンター(研究所), 発がん制御研究部, 室長 (20393126)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Plk1 / 14-3-3ガンマ / チェックポイント / Chk1 / 14-3-3 / 分裂期 / Cdc25A / DNA障害チェックポイント / Cdk1 / 細胞周期 / キナーゼ / リンヒ酸化反応

Research Abstract

Plk1 is known as one of mitotic kinases but its regulation remains largely unknown. In this study, we have demonstrated novel inverse relationship between Plk1 and checkpoint kinase 1(Chk1). We have also identified 14-3-3 gamma as a binding protein for Chk1 and Plk1. The binding between Chk1 and Plk1 is critical for cell cycle arrest in DNA damage response. Now, we are elucidating the significance of the binding between Plk1 and 14-3-3 gamma.

Research Products

• [Journal Article] Novel regulation of checkpoint kinase 1 : Is checkpoint kinase 1 a good candidate for anti-cancer therapy? (2012)
  • Author(s): Goto, H., Izawa, I., Li, P. and Inagaki, M.
  • Journal Title: Cancer Sci. Volume: (in press) Issue: 7 Pages: 1195-1200
  • DOI: 10.1111/j.1349-7006.2012.02280.x
  • Peer Reviewed
• [Journal Article] Trichoplein and Aurora A block aberrant primary cilia assembly in proliferating cells. (2012)
  • Author(s): Inoko, A., …., Inagaki, M.
  • Journal Title: J. Cell Biol. Volume: 197 Issue: 3 Pages: 391-405
  • DOI: 10.1083/jcb.201106101
  • Peer Reviewed
• [Journal Article] P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferation (2012)
  • Author(s): Li, P., Goto, H., Kasahara, K., Matsuyama, M., Wang, Z., Yatabe, Y., Kiyono, T., Inagaki, M.
  • Journal Title: Mol. Biol. Cell Volume: 23 Issue: 8 Pages: 1582-1592
  • DOI: 10.1091/mbc.e11-10-0883
  • Peer Reviewed
• [Journal Article] Nuclear Chk1 prevents premature mitotic entry (2011)
  • Author(s): Matsuyama, M., Goto, H., Kasahara, K., Kawakami, Y., Nakanishi, M., Kiyono, T., Goshima, N., Inagaki, M.
  • Journal Title: J. Cell Sci. Volume: 124 Issue: 13 Pages: 2113-2119
  • DOI: 10.1242/jcs.086488
  • Peer Reviewed
• [Journal Article] Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein (2011)
  • Author(s): Ibi, M., Zou, P., Inoko, A., Shiromizu, T., Matsuyama, M., Hayashi, Y., Enomoto, M., Mori, D., Hirotsune, S., Kiyono, T., Tsukita, S., Goto, H.. Inagaki, M.
  • Journal Title: J. Cell Sci. Volume: 124 Issue: 6 Pages: 857-864
  • DOI: 10.1242/jcs.075705
  • Peer Reviewed
• [Journal Article] Nuclear Chk1 prevents premature mitotic entry (2011)
  • Author(s): Matsuyama M., et al.
  • Journal Title: Journal of Cell Science Volume: (In press)
  • Peer Reviewed
• [Journal Article] Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein (2011)
  • Author(s): Ibi, M., et al.
  • Journal Title: Journal of Cell Science Volume: 124 Pages: 857-864
  • Peer Reviewed
• [Journal Article] 14-3-3γ mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage (2010)
  • Author(s): Kasahara, K., Goto, H., Enomoto, M., Tomono, Y., Kiyono, T., Inagaki, M.
  • Journal Title: EMBO J. Volume: 29 Issue: 16 Pages: 2802-2812
  • DOI: 10.1038/emboj.2010.157
  • Peer Reviewed
• [Journal Article] DNA lesions induced by replication stress trigger mitotic aberration and tetraploidy development (2010)
  • Author(s): Ichijima, Y., Yoshioka, K., Yoshioka, Y., Shinohe, K., Fujimori, H., Unno, J., Takagi, M., Goto, H., Inagaki, M., Mizutani, S., Teraoka, H.
  • Journal Title: PLoS ONE Volume: 5 Issue: 1 Pages: e8821-e8821
  • DOI: 10.1371/journal.pone.0008821
  • Peer Reviewed
• [Journal Article] 14-3-3γ mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage (2010)
  • Author(s): Kasahara K., et al.
  • Journal Title: The EMBO Journal Volume: 29 Pages: 2802-2812
  • Peer Reviewed
• [Journal Article] DNA Lesions Induced by Replication Stress Trigger Mitotic Aberration and Tetraploidy Development. (2010)
  • Author(s): Yousuke Ichijima, et al.
  • Journal Title: PLoS ONE 5
  • Peer Reviewed
• [Journal Article] Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during the G2/M transition (2009)
  • Author(s): Enomoto, M., Goto, H., Tomono, Y., Kasahara, K., Tsujimura, K., Kiyono, T., Inagaki, M.
  • Journal Title: J. Biol. Chem. Volume: 284 Issue: 49 Pages: 34223-34230
  • DOI: 10.1074/jbc.c109.051540
  • Peer Reviewed
• [Journal Article] Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during G2/M transition. (2009)
  • Author(s): Masato Enomoto, et al.
  • Journal Title: J.Biol.Chem. 284 Pages: 34223-34230
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 笠原広介, 他
  • Journal Title: 蛋白質核酸酵素 染色体サイクル(2009年増刊号;54巻5号)(共立出版) Pages: 441-446
• [Presentation] 新規リン酸化反応による分裂期キナーゼPlk1の活性制御メカニズム (2011)
  • Author(s): 笠原広介、後藤英仁、井澤一郎、渡辺信元、清野透、稲垣昌樹
  • Organizer: 第34日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] 新規リン酸化反応による分裂期キナーゼPlk1の活性制御メカニズム (2011)
  • Author(s): 笠原広介, 他
  • Organizer: 第34日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-15
• [Presentation] Chk1 Phosphorylation by p90 Ribosomal S6 Kinase(p90 RSK) (2011)
  • Author(s): Goto H., Li P., Kiyono T., Matsuyama M., Kasahara K., Murakami Y., Yatabe Y., and Inagaki M.
  • Organizer: The 51th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: Denver, CO, USA
  • Year and Date: 2011-12-04
• [Presentation] Trichoplein blocks the abrrant assembly of primary cilia in proliferating cells (2011)
  • Author(s): Inoko A., Matsuyama M., Goto H., Ohmuro-Matsuyama Y., and Inagaki M.
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋国際会議場,名古屋
  • Year and Date: 2011-10-03
• [Presentation] Chk1 phosphorylation at Ser280 by p90 ribosomal S6 kinase(p90 RSK) (2011)
  • Author(s): Goto H., Li P., Yatabe Y., Kasahara K., Matsuyama M., Kiyono T., and Inagaki M
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋国際会議場,名古屋
  • Year and Date: 2011-10-03
• [Presentation] Nuclear Chk1 prevents premature activation of cyclinB1-Cdk1 (2010)
  • Author(s): Matsuyama M., Kasahara K., and Goto H.
  • Organizer: MEXT Priority Research Project "Cell Proliferation Control" International Symposium
  • Place of Presentation: Rubura Ohzan Hotel(Nagoya)
  • Year and Date: 2010-11-06
• [Presentation] 抗リン酸化ペプチド抗体とチェックポイントシグナル (2010)
  • Author(s): 稲垣昌樹、笠原広介、李萍、後藤英仁
  • Organizer: 第74回日本生化学会中部支部例会
  • Place of Presentation: 名古屋大学医学部,名古屋
  • Year and Date: 2010-05-29
• [Presentation] Regulation of Chk1 by novel phosphorylations ; Perspective of Chk1 as a therapeutic target for cancer (2010)
  • Author(s): Goto H., Enomoto M., Kasahara K., and Inagaki M.
  • Organizer: The 15th Aichi International Cancer Symposium
  • Place of Presentation: Aichi Cancer Center, Nagoya
  • Year and Date: 2010-03-06
• [Presentation] Regulation of Chk1 by novel phosphorylations ; Perspective of Chk1 as atherapeutic target for cancer (2010)
  • Author(s): Hidemasa Goto, et al.
  • Organizer: The 15th Aichi International Cancer Symposium
  • Place of Presentation: 愛知県がんセンター(名古屋)
  • Year and Date: 2010-03-06
• [Presentation] Chk1 phosphorylation by Cdk1 is required for the adequate activation of Cdk1 (2009)
  • Author(s): Enomoto M., Goto H., Tomono Y., Kasahara K., Tsujimura K., Kiyono T., and Inagaki M.
  • Organizer: The 49th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: San Diego Convention Center, San Diego, CA, USA
  • Year and Date: 2009-12-08
• [Presentation] 14-3-3γmediates Cdc25A proteolysis to induce S and G2 arrest after DNA damage (2009)
  • Author(s): Goto H., Kasahara K., Tomono Y., Enomoto M., Kiyono T., and Inagaki M.
  • Organizer: The 49th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: San Diego Convention Center, San Diego, CA, USA
  • Year and Date: 2009-12-07
• [Presentation] G2/M期におけるCdk1によるChk1の制御機構の解析 (2009)
  • Author(s): 榎本将人、後藤英仁、友野靖子、笠原広介、辻村邦夫、清野透、稲垣昌樹
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド,神戸
  • Year and Date: 2009-10-23
• [Presentation] Multi-site phosphorylations by different kinases lead to entire function of Chk1 (2009)
  • Author(s): 稲垣昌樹、笠原広介、後藤英仁
  • Organizer: 第68回日本癌学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-02
• [Presentation] Chk1 phosphorylation by Cyclin-dependentkinases(Cdks) promotes mitotic entry.Cell Cycle and Cell Architecture (2009)
  • Author(s): Goto H. and Enomoto M.
  • Organizer: MEXT Priority Research Project"Cell Proliferation Control" International Symposium
  • Place of Presentation: Rubura Ohzan Hotel, Nagoya
  • Year and Date: 2009-02-26
• [Book] チェックポイントキナーゼ1(Chk1)の新規制御機構とがん-シグナル伝達研究最前線2012-井上純一郎、武川睦寛、徳永文稔、今井浩三編実験医学増刊号(30巻, 5号) (2012)
  • Author(s): 後藤英仁、稲垣昌樹
  • Publisher: 羊土社
• [Book] チェックポイントキナーゼ1(Chk1)の新規制御機構とがん-シグナル伝達研究最前線2012-実験医学増刊号(30巻,5号)(井上純一郎、武川睦寛、徳永文稔、今井浩三編) (2012)
  • Author(s): 後藤英仁, 他
  • Publisher: 羊土社
• [Book] G2/M移行期におけるCDK1の活性化およびチェックポイント解除機構-細胞周期フロンティア-佐方功幸、稲垣昌樹、岸本健雄編 (2010)
  • Author(s): 後藤英仁、稲垣昌樹
  • Publisher: 共立出版
• [Book] 細胞周期フロンティア(佐方功幸、稲垣昌樹、岸本健雄編) (2010)
  • Author(s): 後藤英仁, 他
  • Publisher: 共立出版
• [Book] リン酸化によるM期制御-染色体サイクルゲノムの恒常性維持、継承とダイナミックス-正井久雄、升方久夫、釣本敏樹、仁木宏典、篠原彰編,蛋白質核酸酵素増刊号(54巻, 4号) (2009)
  • Author(s): 笠原広介、後藤英仁
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/ri/01bumon/08hatsugan_seigyo/index.html
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/ri/01bumon/08hatsugan_seigyo/index.html