Identification of placenta specific small RNA

• Project/Area Number: 21590364
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human genetics
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: HATA Kenichiro 独立行政法人国立成育医療研究センター, 周産期病態研究部, 部長 (60360335)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 胎盤 / small RNA / エピジェネティクス / TS細胞 / DNAメチル化

Research Abstract

We prepared total RNA from several trophoblast stem cell lines under FGF4 contained medium condition to keep undifferentiated state. Then collected total RNA were purified by column and polyacrylamide gel electrophoresis to pick up highly purified 20-40 mer small RNA fractions. These purified small RNA molecules were ligated with special adaptors and then reveres transcription and PCR were done with adapter-ligated RNA molecules to make the small RNA libraries of torophoblast stem cell lines. The libraries were sequenced and analyzed by small RNA micro arrays. Sequencing and microarray data showed some miRNA families were expressed in both torophoblast stem cells and placental tissues. A part of miRNAs are derived from a miRNA cluster in extraembryonic tissues of E9.5 specifically. Our finding suggests that the miRNAs could be regulated uniquely in mouse development.

Research Products

• [Journal Article] 胎盤形成のエピジェネティクス (2011)
  • Author(s): 秦健一郎
  • Journal Title: 臨床産婦人科 Volume: 65 Pages: 243-247
  • NAID: 10031166251
• [Journal Article] Dynamic stage-specific changes in imprinted differentially methylated regions during early mammalian development and prevalence of non-CpG methylation in oocytes (2010)
  • Author(s): Tomizawa S, et al
  • Journal Title: Development Volume: 138 Pages: 811-820
• [Journal Article] Dynamic stage-specific changes in imprinted differentially methylated regions during early mammalian development and prevalence of non-CpG methylation in oocytes. (2010)
  • Author(s): Tomizawa S, et al.
  • Journal Title: Development Volume: 138 Pages: 811-820
  • Peer Reviewed
• [Journal Article] Histone methylation is mechanistically linked to DNA methylation at imprinting control regions in mammals (2009)
  • Author(s): Henckel, A., et al
  • Journal Title: Hum Mol Genet Volume: 18 Pages: 3375-3383
• [Journal Article] Histone methylation is mechanistically linked to DNA methylation at imprinting control regions in mammals (2009)
  • Author(s): Henckel, A., et al.
  • Journal Title: Hum Mol Genet. 18 Pages: 3375-3383
  • Peer Reviewed
• [Presentation] 発育不全とエピジェネティクス群馬大学生体調節研究所内分泌 (2012)
  • Author(s): 秦健一郎
  • Organizer: 代謝学共同研究拠点シンポジウム
  • Place of Presentation: 前橋
  • Year and Date: 2012-01-12
• [Presentation] 発育不全とエピジェネティクス (2012)
  • Author(s): 秦健一郎
  • Organizer: 群馬大学生体調節研究所内分泌・代謝学共同研究拠点シンポジウム
  • Place of Presentation: 前橋市
  • Year and Date: 2012-01-12
• [Presentation] ジェネティクスを越えてエピジェネティクスへ-周産期のエピジェネティクス (2011)
  • Author(s): 秦健一郎
  • Organizer: 遺伝医学合同学術集会
  • Place of Presentation: 京都
  • Year and Date: 2011-06-17
• [Presentation] ジェネティクスを越えてエピジェネティクスへ-周産期のエピジェネティクス (2011)
  • Author(s): 秦健一郎
  • Organizer: 遺伝医学合同学術集会2011
  • Place of Presentation: 京都
  • Year and Date: 2011-06-17