Elucidation of genomic aberrations involved in recurrence and metastasis of renal cell carcinoma

• Project/Area Number: 21590373
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Oita University
• Principal Investigator: MATSUURA Keiko 大分大学, 医学部, 准教授 (00291542)
• Co-Investigator(Kenkyū-buntansha): MORIYAMA Masatsugu 大分大学, 医学部, 教授 (90239707)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: アレイCGH / 腎細胞癌 / 再発 / メチル化 / ゲノム異常

Research Abstract

We analyzed primary and metastatic lesions of renal cell carcinoma(RCC) by array based comparative genomic hybridization(CGH), using genomic DNA extracted from formalin-fixed, paraffin-embedded tissue samples. We identified some different genomic aberrations between two groups. Next, we analyzed the methylation profiles of promoter regions, and also examined gene expression profiles. As a result of these findings, we found that the patients with 9p loss in primary tumors showed significantly lower recurrence(metastasis) free survival rate than the patients without 9p loss. Two genes located in 9p were identified as a candidate gene associated with recurrence and metastasis in RCC.

Research Products

• [Journal Article] Genomic profiling of renal cell carcinoma in patients with end-stage renal disease (2012)
  • Author(s): Inoue T, Matsuura K, Yoshimoto T, Nguyen LT, Tsukamoto Y, Nakada C, HijiyaN, Narimatsu T, Nomura T, Sato F, Nagashima Y, Kashima K, Hatakeyama S, Ohyama C, Numakura K, Habuchi T, Nakagawa M, Seto M, Mimata H, Moriyama M.
  • Journal Title: Cancer Science Volume: 103(3) Pages: 569-576
• [Journal Article] Downregulation of SAV1 plays a role in pathogenesis of high-grade clear cell renal cell carcinoma (2011)
  • Author(s): Matsuura K, Nakada C, Mashio M, Narimatsu T, Yoshimoto T, Tanigawa M, Tsukamoto Y, Hijiya N, Takeuchi I, Nomura T, Sato F, Mimata H, Seto M, Moriyama M.
  • Journal Title: BMC cancer Volume: 11(1) Pages: 1-10
• [Journal Article] Genomic profiling of submucosal-invasive gastric cancer by array-based comparative genomic hybridization (2011)
  • Author(s): Kuroda A, Tsukamoto Y, Nguyen LT, Noguchi T, TakeuchiI, Uchida M, Uchida T, Hijiya N, Nakada C, Okimoto T, Kodama M, Murakami K, Matsuura K, Seto M, Ito H, Fujioka T, Moriyama M.
  • Journal Title: PloS one Volume: 6(7) Pages: 1-11
• [Journal Article] Overexpression of miR-210, a downstream molecule of HIF1α, causes centrosome amplification in renal carcinoma cells (2011)
  • Author(s): Nakada C, Tsukamoto Y, Matsuura K, Nguyen TL, HijiyaN, Uchida T, Sato F, Mimata H, Seto M, Moriyama M.
  • Journal Title: Journal of Pathology Volume: 224 Pages: 280-288
• [Journal Article] MicroRNA-375 is down-regulated in gastric carcinomas and regulates cell survival by targeting PDK1 and 14-3-3zeta (2010)
  • Author(s): Tsukamoto Y, Nakada C, Noguchi T, Tanigawa M, Tung N, Uchida T, Hijiya N, Matsuura K, Fujioka T, Seto M, Moriyama M.
  • Journal Title: Cancer Research Volume: 70 Pages: 2339-2349
• [Journal Article] Genomic profiling of gastric carcinoma in situ and adenomas by array-based comparative genomic hybridization (2010)
  • Author(s): Uchida M, Tsukamoto Y, Uchida T, Ishikawa Y, Nagai T, Hijiya N, Tung N, Nakada C, Kuroda A, Okimoto T, Kodama M, Murakami K, Noguchi T, Matsuura K, Tanigawa M, Seto M, Ito H, Fujioka T, Takeuchi I, Moriyama M.
  • Journal Title: Journal of Pathology Volume: 221 Pages: 96-105
• [Journal Article] Analysis of p53 mutations and the expression of p53 and p21WAF1/CIP1 protein in 15 cases of sebaceous carcinoma of the eyelid (2010)
  • Author(s): Kiyosaki K, Nakada C, Hijiya N, Tsukamoto Y, Matsuura K, Nakatsuka K, Daa T, Yokoyama S, Imaizumi M, Moriyama M.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 51(1) Pages: 7-11
• [Journal Article] Genomic profiling of gastric carcinoma in situ and adenomas by array-based comparative genomic hybridization. (2010)
  • Author(s): Uchida M, Tsukamoto Y, Uchida T, Ishikawa Y, Nagai T, Hijiya N, Tung N, Nakada C, Kuroda A, Okimoto T, Kodama M, Murakami K, Noguchi T, Matsuura K, Tanigawa M, Seto M, Ito H, Fujioka T, Takeuchi I, Moriyama M.
  • Journal Title: Journal of Pathology Volume: 221 Pages: 96-105
  • Peer Reviewed
• [Journal Article] MicroRNA-375 is down-regulated in gastric carcinomas and regulates cell survival by targeting PDK1 and 14-3-3ζ. (2010)
  • Author(s): Tsukamoto Y, Nakada C, Noguchi T, Tanigawa M, Tung N, Uchida T, Hijiya N, Matsuura K, Fujioka T, Seto M, Moriyama M.(equal contribution)
  • Journal Title: Cancer Research Volume: 70 Pages: 2339-2349
  • Peer Reviewed
• [Journal Article] Analysis of p53 mutations and the expression of p53 and p21 WAF1/CIP1 protein in 15 cases of sebaceous carcinoma of the eyelid (2010)
  • Author(s): Kiyosaki K, Nakada C, Hijiya N, Tsukamoto Y, Matsuura K, Nakatsuka K, Daa T, Yokoyama S, Imaizumi M, Moriyama M.
  • Journal Title: Invest Ophthalmol Vis Sci. 51(1) Pages: 7-11
  • Peer Reviewed
• [Presentation] 網羅的ゲノム解析による腎癌転移関連遺伝子の同定と解析 (2012)
  • Author(s): 成松隆弘, 松浦恵子, 泥谷直樹, 中田知里, 井上亨, 野村威雄, 竹内一郎, 瀬戸加大, 佐藤文憲, 守山正胤, 三股浩光
  • Organizer: 第21回泌尿器科分子細胞研究会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2012-02-11
• [Presentation] 集合管癌の臨床病理学的検討 (2011)
  • Author(s): 井上享, 松浦恵子, 長嶋洋治, 成松隆弘, 野村威雄, 佐藤文憲, 守山正胤, 三股浩光
  • Organizer: 第63回日本泌尿器科学会西日本総会
  • Place of Presentation: 福岡県久留米市
  • Year and Date: 2011-11-05
• [Presentation] 9p24. 1-p13. 3 loss may be indicator to predict metastasis of clear cell renal cell carcinoma (2011)
  • Author(s): Narimatsu T, Matsuura K, Hijiya N, Nakada C, Inoue T, Nomura T, Sato F, Seto M, Mimata H, Moriyama M
  • Organizer: 70th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2011-10-03
• [Presentation] Frequent deregulation of the Hippo signaling pathway in high-grade clear cell renal cell carcinoma (2011)
  • Author(s): Matsuura K, Nakada C, Narimatsu T, Tsukamoto Y, Nomura T, Sato F, Mimata H, Seto M, Moriyama M
  • Organizer: 70th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2011-10-03
• [Presentation] Frequent deregulation of the Hippo signaling pathway in high-grade clear cell renal cell carcinoma (2011)
  • Author(s): Matsuura K
  • Organizer: 70^<th> Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2011-10-03
• [Presentation] 9p24.1-p13.3 loss may be indicator to predict metastasis of clear cell renal cell carcinoma (2011)
  • Author(s): Narimatsu T
  • Organizer: 70^<th> Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2011-10-03
• [Presentation] GENOMIC COPY NUMBER LOSS AT 9P IS ASSOCIATED WITH METASTASIS OF RENAL CELL CARCINOMA (2011)
  • Author(s): Narimatsu T, Matsuura K, Nakada C, Inoue T, NomuraT, Sato F, Moriyama M, Mimata H
  • Organizer: 2011 AUA Annual Meeting
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2011-05-16
• [Presentation] GENOMIC PROFILING OF RENAL CELL CARCINOMA IN PATIENTS WITH END-STAGE RENAL DISEASE (2011)
  • Author(s): Inoue T, Matsuura K, Yoshimoto T, Narimatsu T, Sato F, Nagashima Y, Hatakeyama S, Ohyama C, Habuchi T, Nakagawa M, Moriyama M, Mimata H
  • Organizer: 2011 AUA Annual Meeting
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2011-05-15
• [Presentation] Array CGH analysis of renal cell carcinomas in patients withend-stage renal disease (2011)
  • Author(s): 井上享, 松浦恵子, 吉本多一郎, 成松隆弘, 長嶋洋治, 畠山真吾, 佐藤文憲, 大山力, 羽渕友則, 中川昌之, 三股浩光, 守山正胤
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2011-04-23
• [Presentation] 腎癌転移と9pゲノムコピー数異常の関係 (2011)
  • Author(s): 成松隆弘, 松浦恵子, 井上享, 吉本多一郎, 中田知里, 竹内一郎, 瀬戸加大, 佐藤文憲, 三股浩光, 守山正胤
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2011-04-23
• [Presentation] Array CGH analysis of renal cell carcinomas in patients with end-stage renal disease (2011)
  • Author(s): 井上享
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2011-04-23
• [Presentation] 腎癌転移と9pゲノムコピー数異常の関係 (2011)
  • Author(s): 成松隆弘, 松浦恵子, 中田知里, 井上亨, 野村威雄, 佐藤文憲, 守山正胤, 三股浩光
  • Organizer: 第20回分子細胞研究会
  • Place of Presentation: 三重県津市
  • Year and Date: 2011-03-12
• [Presentation] Array CGH analysis of renal cell carcinomas in patients withend-stage renal disease (2010)
  • Author(s): Inoue T, Yoshimoto T, Matsuura K, Narimatsu T, Nakada C, Nagashima Y, Hatakeyama S, Sato F, Oyama C, Habuchi T, Nakagawa M, Moriyama M, Mimata H
  • Organizer: 第62回日本泌尿器科学会西日本総会
  • Place of Presentation: 鹿児島県鹿児島市
  • Year and Date: 2010-11-05
• [Presentation] Array CGH analysis of renal cell carcinomas in patients with end-stage renal disease (2010)
  • Author(s): Inoue T, Yoshimoto T, Matsuura K, Narimatsu T, Nakada C, Nagashima Y, Hatakeyama S, Sato F, Oyama C, Habuchi T, Nakagawa M, Moriyama M, Mimata H
  • Organizer: 第62回日本泌尿器科学会西日本総会
  • Place of Presentation: 鹿児島県鹿児島市
  • Year and Date: 2010-11-05
• [Presentation] Array CGH analysis of renal cell carcinomas in patients with end-stage renal disease (2010)
  • Author(s): Inoue T, Yoshimoto T, Narimatsu T, Nagashima Y, Matsuura K, Hatakeyama S, Sato F, Ohyama C, Habuchi T, Nakagawa M, Moriyama M, Mimata H
  • Organizer: 69th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2010-09-24
• [Presentation] Array CGH analysis of renal cell carcinomas in patients with end-stage renal disease (2010)
  • Author(s): Inoue T, Yoshimoto T, Narimatsu T, Nagashima Y, Matsuura K, Hatakeyama S, Sato F, Ohyama C, Habuchi T, Nakagawa M, Moriyama M, Mimata H
  • Organizer: 69^<th> Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2010-09-24
• [Presentation] Genomic copy number loss at 9p is associated with metastasis of renal cell carcinoma (2010)
  • Author(s): Narimatsu T, Inoue T, Yoshimoto T, Nakada C, Matsuura K, Sato F, Seto M, Mimata H, Moriyama M
  • Organizer: 69th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2010-09-23
• [Presentation] Genomic copy number loss at 9p is associated with metastasis of renal cell carcinoma. (2010)
  • Author(s): Narimatsu T, Inoue T, Yoshimoto T, Nakada C, Matsuura K, Sato F, Seto M, Mimata H, Moriyama M
  • Organizer: 69^<th> Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2010-09-23
• [Presentation] 転移性腎癌におけるゲノムコピー数異常の増加 (2010)
  • Author(s): 成松隆弘, 井上享, 吉本多一郎, 中田知里, 松浦恵子, 守山正胤, 瀬戸加大, 佐藤文憲, 三股浩光
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 岩手県盛岡市
  • Year and Date: 2010-04-29
• [Presentation] 透析腎癌の網羅的ゲノム解析 (2010)
  • Author(s): 井上享, 吉本多一郎, 成松隆弘, 中田知里, 松浦恵子, 守山正胤, 瀬戸加大, 長嶋洋治, 佐藤文憲, 羽渕友則, 三股浩光
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 岩手県盛岡市
  • Year and Date: 2010-04-28
• [Presentation] ベリニ管癌の網羅的ゲノム解析 (2010)
  • Author(s): 井上享, 成松隆, 中田知里, 松浦恵子, 佐藤文, 長嶋洋治, 矢尾正祐, 窪田吉信, 羽渕友則, 大山力, 西山博之, 小川修, 中川昌之, 守山正胤, 三股浩光
  • Organizer: 第19回泌尿器科分子・細胞研究会
  • Place of Presentation: 神戸市
• [Presentation] Increased DNA Copy Number Alterations in Metastatic Renal Cell Carcinoma (2009)
  • Author(s): Narimatsu T, Inoue T, Yoshimoto T, Nakada C, Matsuura K, Moriyama M, Seto M, Sato F, Mimata H
  • Organizer: 第96回日本泌尿器科学会西日本総会
  • Place of Presentation: 高松市
• [Presentation] 腎細胞癌の網羅的DNAメチル化異常の解析 (2009)
  • Author(s): 井上享, 成松隆, 吉本多一郎, 中田知里, 松浦恵子, 守山正胤, 佐藤文憲, 三股浩光
  • Organizer: 第97回日本泌尿器科学会総会
  • Place of Presentation: 岡山市
• [Presentation] Array CGHによる腎癌転移巣の網羅的解析 (2009)
  • Author(s): 成松隆弘, 井上享, 吉本多一郎, 中田知里, 松浦恵子, 守山正胤, 瀬戸加大, 佐藤文憲, 三股浩光
  • Organizer: 第97回日本泌尿器科学会総会
  • Place of Presentation: 岡山市