Genome-wide microRNA expression profiling in gastric cancer

• Project/Area Number: 21590398
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Oita University
• Principal Investigator: MORIYAMA Masatsugu 大分大学, 医学部, 教授 (90239707)
• Co-Investigator(Kenkyū-buntansha): TSUKAMOTO Yoshiyuki 大分大学, 医学部, 助教 (00433053)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: マイクロアレイ / 網羅的解析 / 胃癌 / マイクロRNA / microRNA / アポトーシス / アレイCGH / ゲノムコピー数異常 / real-time PCR

Research Abstract

We investigated expression profiles of microRNA(miRNA) in gastric carcinomas by use of a miRNA microarray platform covering a total of 470 human miRNAs. By comparing miRNA expression profiles between intestinal-type and diffuse-type gastric carcinomas(ANOVA P<0. 05), we could not identify any miRNAs differentially expressed between them, suggesting that, in gastric carcinomas, the miRNA expression profile might not be correlated with histopathologic subtypes. We identified 39 differentially expressed miRNAs in gastric carcinoma, of which 6 were significantly downregulated and the other 33 were upregulated. Among the 6 down-regulated miRNA, we selected 3, miR-375, miR-29c and miR-148a, as gastric cancer-related miRNA.

Research Products

• [Journal Article] Overexpression of miR-210, a downstream target of HIF1α, causes centrosome amplification in renal carcinoma cells (2011)
  • Author(s): Nakada C, Tsukamoto Y, Matsuura K, Nguyen TL, Hijiya N, Uchida T, Sato F, Mimata H, Seto M, Moriyama M.
  • Journal Title: J Pathol Volume: 224(2) Pages: 280-8
• [Journal Article] Overexpression of miR-210, a downstream molecule of HIF1α, causes centrosome amplification in renal carcinoma cells. (2011)
  • Author(s): Chisato Nakada, Yoshiyuki Tsukamoto, Keiko Matsuura, Tung Lam Nguyen, Naoki Hijiya, Tomohisa Uchida, Fuminori Sato, Hiromitsu Mimata, Seto Masao, Masatsugu Moriyama
  • Journal Title: Journal of Pathology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] MicroRNA-375 is downregulated in gastric carcinomas and regulates cell survival by targeting PDK1 and 14-3-3zeta (2010)
  • Author(s): Tsukamoto Y, Nakada C, Noguchi T, Tanigawa M, Nguyen LT, Uchida T, Hijiya N, Matsuura K, Fujioka T, Seto M, Moriyama M
  • Journal Title: Cancer Res Volume: 70(6) Pages: 2339-49
• [Journal Article] MicroRNA-375 is down-regulated in gastric carcinomas and regulates cell survival by targeting PDK1 and 14-3-3ζ. (2010)
  • Author(s): Yoshiyuki Tsukamoto, Chisato Nakada, Tsuyoshi Noguchi, Masato Tanigawa, Nguyen Lam Tung, Tomohisa Uchida, Naoki Hijia, Keiko Matsuura, Toshio Fujioka, Masao Seto, Masatsugu Moriyama
  • Journal Title: Cancer Research Volume: 70 Pages: 2339-2349
  • Peer Reviewed
• [Journal Article] MicroRNA-375 is down-regulated in gastric carcinomas and regulates cell survival by targeting PDK1 and 14-3-3ζ (2010)
  • Author(s): Yoshiyuki Tsukamoto, Chisato Nakada, Tsuyoshi Noguchi, Masato Tanigawa, Nguyen Lam Tung, Tomohisa Uchida, Naoki Hijiya, Keiko Matsuura, Toshio Fujioka, Masao Seto, Masatsugu Moriyama
  • Journal Title: Cancer Research 70 Pages: 2339-2349
  • Peer Reviewed
• [Journal Article] Genomic profiling of gastric carcinoma in situ and adenomas by array-based comparative genomic hybridization (2010)
  • Author(s): Masahiro Uchida, Yoshiyuki Tsukamoto, Tomohisa Uchida, Yuta Ishikawa, Takayuki Nagai, Naoki Hijiya, Lam Tung Nguyen, Chisato Nakada, Akiko Kuroda, Tadayoshi Okimoto, Masaaki Kodama, Kazunari Murakami, Tsuyoshi Noguchi, Keiko Matsuura, Masato Tanigawa, Masao Seto, Hisao Ito, Toshio Fujioka, Ichiro Takeuchi, Masatsugu Moriyama
  • Journal Title: Journal of Pathology 221 Pages: 96-105
  • Peer Reviewed
• [Journal Article] Genome-wide microRNA expression profiling in renal cell carcinoma : significant down-regulation of miR-141 and miR-200c (2008)
  • Author(s): Nakada C, Matsuura K, Tsukamoto Y, Tanigawa M, Yoshimoto T, Narimatsu T, Nguyen LT, Hijiya N, Uchida T, Sato F, Mimata H, Seto M, Moriyama M
  • Journal Title: J Pathol Volume: 216(4) Pages: 418-27
• [Presentation] 胃癌におけるマイクロRNA発現 (2011)
  • Author(s): 塚本善之、守山正胤
  • Organizer: 第100回日本病理学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-04-28
• [Presentation] 胃癌におけるマイクロRNA発現 (2011)
  • Author(s): 塚本善之
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜(招待講演)
  • Year and Date: 2011-04-28
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/byori2/