| Project/Area Number |
21590398
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Oita University |
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Principal Investigator |
|
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| Co-Investigator(Kenkyū-buntansha) |
TSUKAMOTO Yoshiyuki 大分大学, 医学部, 助教 (00433053)
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|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | マイクロアレイ / 網羅的解析 / 胃癌 / マイクロRNA / microRNA / アポトーシス / アレイCGH / ゲノムコピー数異常 / real-time PCR |
|---|
| Research Abstract |
We investigated expression profiles of microRNA(miRNA) in gastric carcinomas by use of a miRNA microarray platform covering a total of 470 human miRNAs. By comparing miRNA expression profiles between intestinal-type and diffuse-type gastric carcinomas(ANOVA P<0. 05), we could not identify any miRNAs differentially expressed between them, suggesting that, in gastric carcinomas, the miRNA expression profile might not be correlated with histopathologic subtypes. We identified 39 differentially expressed miRNAs in gastric carcinoma, of which 6 were significantly downregulated and the other 33 were upregulated. Among the 6 down-regulated miRNA, we selected 3, miR-375, miR-29c and miR-148a, as gastric cancer-related miRNA.
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