Roles of SIX3 and SIL in the Hedgehog signaling during pancreatic carcinogenesis.

• Project/Area Number: 21590408
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Aichi Medical University
• Principal Investigator: KASAI Kenji 愛知医科大学, 医学部, 准教授 (70242857)
• Co-Investigator(Renkei-kenkyūsha): IKEDA Hiroshi 愛知医科大学, 医学部, 教授 (00131219) ; INAGUMA Shingo 愛知医科大学, 医学部, 講師 (80410786)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ヘッジホッグ / 膵臓癌 / 前癌病変 / SIX3 / SIL

Research Abstract

SIX3 protein was expressed in the normal duct cells of the pancreas whereas not in PanIN/cancer cells. Human SIX3 promoter contained a CpG island which was methylated in SIX3(-) pancreatic cancer cell lines. Reporter assay revealed roles of SIX3 and TLE1/HDAC1 in the MACS1-dependent suppression of human Shh gene. SIL was strongly expressed in PanIN/cancer cells of the pancreas. It was also weakly expressed at the basal of the normal duct cells of the pancreas, indicating its role for the cellular attachment to the matrix.

Research Products

• [Journal Article] GLI1 modulates EMT in pancreatic cancer-Letter (2012)
  • Author(s): Shingo Inaguma, Kenji Kasai, Mitsuyoshi Hashimoto, Hiroshi Ikeda
  • Journal Title: Cancer Research Volume: (in press) Issue: 14 Pages: 3702-3
  • DOI: 10.1158/0008-5472.can-12-0379
  • Peer Reviewed
• [Journal Article] GLI1 facilitates the migration and invasion of pancreatic cancer cells through MUC5AC-mediated attenuation of E-cadherin (2011)
  • Author(s): Shingo Inaguma, Kenji Kasai, Hiroshi Ikeda
  • Journal Title: Oncogene Volume: 30 Issue: 6 Pages: 714-723
  • DOI: 10.1038/onc.2010.459
  • Peer Reviewed
• [Journal Article] GLI1 facilitates the migarion and invasion of pancreatic cancer cellls through MUC5AC-mediated attenuation of E-cadherin. (2011)
  • Author(s): S Inaguma, K Kasai, H Ikeda.
  • Journal Title: Oncogene Volume: 30巻 Pages: 714-723
  • Peer Reviewed
• [Presentation] 胃小細胞癌の一例:遺伝子変異LOH解析を含めた検討 (2011)
  • Author(s): 稲熊真悟、笠井謙次、池田洋
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-04-29
• [Presentation] 胃小細胞癌の一例遺伝子変異・LOH解析を含めた検討 (2011)
  • Author(s): 稲熊真悟、笠井謙次、池田洋
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)(一般示説)
  • Year and Date: 2011-04-29
• [Presentation] GLI1はMUC5ACの発現誘導によってE-cadherinによる細胞間接着を阻害し膵癌細胞の遊走・浸潤を促進する (2010)
  • Author(s): 稲熊真悟、笠井謙次、米山亜紀子、池田洋
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-04-29
• [Presentation] GLI1 enhances themotility and invasion by gel forming mucin MUC5AC up-regulation in pancreatic cancer cells (2009)
  • Author(s): 稲熊真悟、笠井謙次
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-10-01
• [Presentation] GLI1 enhances the motility and invasion by gel forming mucin MUC5AC up-regulation in pancreatic cancer cells (2009)
  • Author(s): 稲熊真悟
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフイコ横浜(横浜市)
  • Year and Date: 2009-10-01
• [Presentation] 膵臓癌およびPancreatic Intraepithelial Neoplasia(PanIN)におけるSIL発現状況の免疫組織学的解析 (2009)
  • Author(s): 米山亜紀子、稲熊真悟、笠井謙次、池田洋
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2009-05-03
• [Presentation] 膵臓癌においてGLI1はbHLH型転写抑制因子DEC2の発現を直接誘導しMLH1を介した遺伝子不安定性に寄与する (2009)
  • Author(s): 稲熊真悟、笠井謙次、米山亜紀子、池田洋
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2009-05-03
• [Presentation] 膵臓癌および Pancreatic Intraepithelial Neoplasia(PanIN)におけるSIL発現状況の免疫組織学的解析 (2009)
  • Author(s): 米山亜紀子
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2009-05-03
• [Presentation] 膵癌細胞においてGLI1はbHLH型転写抑制因子DEC2の発現を直接誘導しMLH1を介した遺伝子不安定性に寄与する (2009)
  • Author(s): 稲熊真悟
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2009-05-03
• [Remarks]
  • URL: http://www.aichi-med-u.ac.jp/
• [Remarks]
  • URL: http://www.aichi-med-u.ac.jp/