Establishment of method to re-educate mecrophage differentiation towards dendritic cells on the view of regeneration medicine

• Project/Area Number: 21590416
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: University of the Ryukyus
• Principal Investigator: SAIO Masanao 琉球大学, 医学部附属病院, 准教授 (40242721)
• Co-Investigator(Kenkyū-buntansha): TAKAMI Tsuyoshi 岐阜大学, 大学院・医学系研究科, 教授 (70136943) ; YOSHIMI Naoki 琉球大学, 大学院・医学研究科, 教授 (30166996) ; TOMITA Mariko 琉球大学, 大学院・医学研究科, 准教授 (80381250)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 腫瘍内マクロファージ / マウス / 樹状細胞

Research Abstract

Granulocyte macrophage colony stimulating factor(GM-CSF) treatment alone could not alter expression level of macrophage differentiation. On the other hands, when the TIM was treated with GM-CSF and siRNA for macrophage colony stimulating factor(M-CSF) receptor(M-CSFR) altered the expression of signal pathway proteins towards dendritic cell like feature. Above findings indicated we successfully re-educated(re-differentiated) TIM towards monocyte-derived dendritic cells like population and we showed the macrophage plasticity.

Research Products

• [Journal Article] Combined GM-CSF treatment and M-CSF inhibition of tumor-associated macrophages induces dendritic cell-like signaling in vitro (2011)
  • Author(s): Kitoh Y., Saio M., Gotoh N., Umemura N., Nonaka K., Bai J., Vizkeleti L., Torocsik D., Balazs M., Adany R. and Takami T.
  • Journal Title: Int J Oncol Volume: 38 Pages: 1409-19
  • Peer Reviewed
• [Journal Article] Combined GM-CSF treatment and M-CSF inhibition of tumor-associated macrophages induces dendritic cell-like signaling in vitro (2011)
  • Author(s): Kitoh, Y., Saio, M., Gotoh, N., Umemura, N., Nonaka, K., Bai, J., Vizkeleti, L., Torocsik, D., Balazs, M., Adany, R., Takami, T.
  • Journal Title: Int J Oncol Volume: 38 Pages: 1409-1419
  • Peer Reviewed