Observation of in vivo dynamics of macrophage subsets based on expression of lectin molecule

• Project/Area Number: 21590417
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kyoto University
• Principal Investigator: TAKAHARA Kazuhiko 京都大学, 生命科学研究科, 講師 (90301233)
• Co-Investigator(Kenkyū-buntansha): INABA Kayo 京都大学, 生命科学研究科, 教授 (00115792)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞 / レクチン / マクロファージ / 樹状細胞 / 単球

Research Abstract

Dendritic cells/macrophages play important roles in immune system. A part of these cells is derived from monocytes in blood cells generated by bone marrow. However, dynamics of monocytes isn' t fully clear, because of lack of appropriate markers. We found that SIGNR3, a lectin, is a new and useful marker for identification of monocytes. Using a new antibody to SIGNR3, we studied behavior of monocytes in steady and inflammatory states.

Research Products

• [Journal Article] Efficient capture of Calbicans and zymosan by SIGNR1 facilitates TLR2-dependent TNF-αproduction by macrophages (2012)
  • Author(s): Takahara, K., Tokieda, S., Nagaoka, K. and Inaba, K
  • Journal Title: Int. Immunol Volume: 24 Pages: 89-96
• [Journal Article] Difference in fine specificity to polysaccharides of C. albicans mannoprotein between mouse SIGNR1 and human. DC-SIGN. (2012)
  • Author(s): Takahara, K., Tokieda, S., Nagaoka, K., Takeda, T., Kimura, Y. and Inaba, K
  • Journal Title: Infect. Immun Volume: 41 Pages: 1435-44
• [Journal Article] Efficient capture of C. albicans and zymosan by SIGNR1 facilitates TLR2-dependent TNF-α production by macrophages (2012)
  • Author(s): Takahara, K.
  • Journal Title: Int.Immunol. Volume: 24 Issue: 2 Pages: 89-96
  • DOI: 10.1093/intimm/dxr103
  • Peer Reviewed
• [Journal Article] Difference in fine specificity to polysaccharides of C.albicans mannoprotein between mouse SIGNR1 and human DC-SIGN (2012)
  • Author(s): Takahara, K.
  • Journal Title: Infect.Immun. Volume: 80 Issue: 5 Pages: 1699-1706
  • DOI: 10.1128/iai.06308-11
  • Peer Reviewed
• [Journal Article] GM-CSF-independent CD1a expression in epidermal Langerhans cells : evidence from human CD1A genome-transgenic mice. (2011)
  • Author(s): Kobayashi, C., Shiina, T., Tokioka, A., Hattori, Y., Komori, T., Kobayashi-Miura, M., Takizawa, T., Takahara, K., Inaba, K., Inoko, H., Takeya, M., Dranoff, G. and Sugita, M.
  • Journal Title: J. Invest. Dermatol. Volume: 132 Pages: 241-244
• [Journal Article] C-type lectin SIGNR1 enhances cellular oxidative burst response against C. albicans in cooperation with Dectin-1. (2011)
  • Author(s): Takahara, K., Tokieda, S., Nagaoka, K., Takeda, T., Kimura, Y. and Inaba, K
  • Journal Title: Eur. J. Immunol Volume: 88 Pages: 913-924
• [Journal Article] GM-CSF-Independent CD1a Expression in Epidermal Langerhans Cells : Evidence from Human CD1A Genome-Transgenic Mice (2011)
  • Author(s): Kobayashi Chisa; Shiina Takashi; Tokioka Atsuko, et al
  • Journal Title: JOURNAL OF INVESTIGATIVE DERMATOLOGY Volume: 132:1 Issue: 1 Pages: 241-244
  • DOI: 10.1038/jid.2011.280
  • Peer Reviewed
• [Journal Article] C-type lectin SIGNR1 enhances cellular oxidative burst response against C.albicans in cooperation with Dectin-1 (2011)
  • Author(s): Kazuhiko Takahara, et al.
  • Journal Title: Eur.J.Immunol. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Expression of C-type lectin, SIGNR3, on subsets of dendritic cells, macrophages and monocytes (2010)
  • Author(s): Nagaoka, K., Takahara, K., Minamino, K., Takeda, T., Yoshida, Y. and Inaba, K
  • Journal Title: J. Leukoc. Biol Volume: 88 Pages: 913-924
• [Journal Article] Expression of C-type lectin, SIGNR3, on subsets of dendritic cells, macrophages, and monocytes (2010)
  • Author(s): Koji Nagaoka, et al.
  • Journal Title: J.Leukoc.Biol. Volume: 81 Pages: 913-924
  • Peer Reviewed
• [Journal Article] Ly49Q plays a crucial role in neutrophil polarization and migration by regulating raft trafficking (2010)
  • Author(s): Shigemi Sasawatari, et al.
  • Journal Title: Immunity Volume: 31 Pages: 200-213
  • Peer Reviewed
• [Journal Article] Dual role for Id2 in chemical carcinogen-induced skin tumorigenesis (2009)
  • Author(s): Atsushi Tokuriki, et al.
  • Journal Title: Carcinogenesis Volume: 30 Pages: 1645-1650
  • Peer Reviewed
• [Journal Article] Spatiotemporal regulation of intracellular trafficking of TLR9 by an inhibitory receptor, Ly49Q (2009)
  • Author(s): Mariko Yoshizaki, et al.
  • Journal Title: Blood Volume: 114 Pages: 1518-1527
  • Peer Reviewed
• [Presentation] 粒子サイズによる細胞応答性の差異-細胞には粒子の大きさに依存した応答経路が存在する (2012)
  • Author(s): 高原和彦
  • Organizer: ナノ材料の安全性・社会受容に関するシンポジウム
  • Place of Presentation: 京都リサーチパーク(京都府)(招待講演)
  • Year and Date: 2012-01-24
• [Presentation] C型レクチンSIGNR1はDectin-1と共同して細胞のoxidative burstを促進する (2011)
  • Author(s): 高原和彦
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張
  • Year and Date: 2011-11-28
• [Presentation] C型レクチンSIGNR1はDectin-1と共同して細胞のoxidative burstを促進する (2011)
  • Author(s): 高原和彦
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-28
• [Presentation] Recognition of Calbicans by SIGNR1 and subsequent responses in vivo (2010)
  • Author(s): Kazuhiko Takahara
  • Organizer: 10th Awaji International Forum for Infection and Immunity
  • Place of Presentation: 淡路
  • Year and Date: 2010-09-09
• [Presentation] Recognition of C.albicans by SIGNR1 and subsequent responses in vivo (2010)
  • Author(s): Kazuhiko Takahara, et al.
  • Organizer: 10th Awaji International Forum for Infection and Immunity
  • Place of Presentation: 淡路
  • Year and Date: 2010-09-09
• [Presentation] Ly6Chigh SIGNR3-monocytes can differentiate to CD11b+ dendritic cells in subcutaneous lymph nodes under steady-state condition. (2010)
  • Author(s): Koji Nagaoka
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-25
• [Presentation] Binding properties of SIGNR1 against surface mannoprotein of Candida albicans. (2010)
  • Author(s): Sumika Tokieda
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-25
• [Presentation] Ly6C^<high>SIGNR3^- monocytes can differentiate to CD 11b+ dendritic cells in subcutaneous lymph nodes under steady-state condition (2010)
  • Author(s): Koji Nagaoka, et.al.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-25
• [Presentation] Binding properties of SIGNR1 against surface mannoprotein of Candida albicans (2010)
  • Author(s): Sumika Tokieda, et.al.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-25
• [Presentation] Regulation of B cell function and development through IRF-2 in type I interferon receptor-dependent and -independent mechanisms (2010)
  • Author(s): Kento Minamino, et.al.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-24
• [Presentation] Determination of polysaccharides on C.albicans mannoprotein recognized by human C-type lectin, DC-SIGN. (2009)
  • Author(s): Kazuhiko Takahara, et al.
  • Organizer: 9th Awaji International Forum for Infection and Immunity
  • Place of Presentation: 淡路
  • Year and Date: 2009-09-08
• [Remarks]
  • URL: http://zoo.zool.kyoto-u.ac.jp/imm/
• [Remarks]
  • URL: http://zoo.zool.kyoto-u.ac.jp/imm/
• [Remarks]
  • URL: http://zoo.zool.kyoto-u.ac.jp/imm/
• [Remarks]
  • URL: http://zoo.zool.kyoto-u.ac.jp/imm/