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Role of gap-junctional coupling between cardiomyocytes and stromal cells in genesis of cardiac arrhythmias

Research Project

Project/Area Number 21590420
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

TANAKA Hideo  京都府立医科大学, 大学院・医学研究科, 准教授 (60236619)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords線維芽細胞 / 心筋梗塞 / ギャップ結合 / 不整脈 / 肉芽組織 / 間質 / 心筋 / 梗塞
Research Abstract

During the healing phase of myocardial infarct, proliferation of endothelial cells and fibroblasts constitute granulation tissue to replace the infarct resion. Recent reports have confirmed that stromal cells, especially fibroblasts, coupled electrically with cardiomyocytes via gap junction proteins connexins, provide an important basis for cardiac arrhythmias.However, it is unresoloved whether or not stromal cells at the border zone of myocardial infarct really contribute to the arrhythmogenesis. To address this, this project was conducted by using an infarct model of conditional knockout mice specific for Cx43 in the Fsp-1 proteins. Continuous ECG recording in combination with histological analysis was conducted in the Fsp-Cre/Cx43 loxP mice. It was found that the heart devoid of Cx43 in the fibroblasts and endothelial cells failed to attenuate ventricular arrhythmias after infarction. However, formation of granulation tissue showed a significant retardation in the Cx43-KO mice after infarction as compared with wild type mice. The results indicate that Cx43 contributes to formation of granulation tissue in the infarct heart.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (28 results)

All 2012 2011 2010 2009

All Journal Article (9 results) (of which Peer Reviewed: 7 results) Presentation (15 results) Book (4 results)

  • [Journal Article] Dual-wavelength excitation of mucosal autofluorescence for precise detection of diminutive colonic adenomas Gastrointest Endocs2012

    • Author(s)
      Imaizumi K, Harada Y, Wakabayashi N, Yamaoka Y, Konishi H, Dai P, Tanaka H, Takamatsu T
    • Journal Title

      Gastrointestinal Endoscopy

      Volume: 75巻 Issue: 1 Pages: 110-117

    • DOI

      10.1016/j.gie.2011.08.012

    • ISSN
      0016-5107
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] promotes cardiac dysfunction in diabetic mellitus due to excessive mitochondrial respiration-mediated ROS generation and lipid accumulation Circ- Heart Fail2012

    • Author(s)
      Nakamura H, Matoba S, Iwai-Kanai E, Kimata M, Hoshino A, Nakaoka M, Katamura M, Okawa Y, Mita Y, Ikeda K, Okigaki M, Adachi S, Tanaka H, Takamatsu T, Matsubara H
    • Journal Title

      Eur Heart J.

      Volume: 5巻 Issue: 1 Pages: 106-115

    • DOI

      10.1161/circheartfailure.111.961565

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dual-wavelength excitation of mucosal autofluorescence for precise detection of diminutive colonic adenomas2012

    • Author(s)
      Imaizumi K, Harada Y, WakabayashiN, Yamaoka Y, Konishi H, Dai P, Tanaka H, Takamatsu T
    • Journal Title

      Gastrointest Endocs

      Volume: 75 Pages: 110-117

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] p53 promotes cardiac dysfunction in diabetic mellitus due to excessive mitochondrial respiration-mediated ROS generation and lipid accumulation2012

    • Author(s)
      Nakamura H, Matoba S, Iwai-Kanai E, Kimata M, Hoshino A, Nakaoka M, Katamura M, Okawa Y, Mita Y, Ikeda K, Okigaki M, Adachi S, Tanaka H, Takamatsu T, Matsubara H
    • Journal Title

      Circ-Heart Fail

      Volume: 5 Pages: 106-115

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dissipation of beta-dystroglycan with resultant hypercontracture of sarcomeres is culprit for formation of contraction bands in rat injured hearts2011

    • Author(s)
      Tanaka H, Mani, H, Adachi T, Jiang Y, Takamatsu T
    • Journal Title

      Eur Heart J

      Volume: 32巻(Suppll) Issue: Supplement 1 Pages: 691-691

    • DOI

      10.1093/eurheartj/ehr324

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dissipation of beta-dystroglyean with resultant hypercontracture of sarcomeres is culprit for formation of contraction bands in rat injured hearts2011

    • Author(s)
      Tanaka H, Mani, H, Adachi T, Jiang Y, Takamatsu T
    • Journal Title

      Eur Heart J

      Volume: 32 Suppl 1 Pages: 691-691

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 心電図マイルストーン 重要論文を解説する2010

    • Author(s)
      田中秀央、高松哲郎
    • Journal Title

      心電図

      Volume: 30 Pages: 86-86

    • Related Report
      2010 Annual Research Report
  • [Journal Article] なぜ心房細動は起こるのか2010

    • Author(s)
      田中秀央
    • Journal Title

      京都府立医科大学雑誌

      Volume: 119 Pages: 261-268

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Intracellular Dynamics of topoisomerase l inhibitor, CPT-11, by slit-scanning confocal Raman microscopy.2009

    • Author(s)
      Harada Y, Dai P, Yamaoka Y, Ogawa M, Tanaka H, Nosaka K, Akaji K, Takamatsu T.
    • Journal Title

      Histochem Cell Biol 132

      Pages: 39-46

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 心筋・線維芽細胞間ギャップ結合コミュニケーションは心筋の興奮伝導を障害する2011

    • Author(s)
      足達哲也、田中秀央、姜艶、橋本恵美、高松哲郎
    • Organizer
      日本組織細胞化学会学術集会
    • Place of Presentation
      金沢市
    • Year and Date
      2011-09-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 虚血・再灌流障害心の収縮帯形成はβジストログリカンの脱落によって生じる2011

    • Author(s)
      田中秀央、足達哲也、姜艶、橋本恵美、高松哲郎
    • Organizer
      日本組織細胞化学会学術集会
    • Place of Presentation
      金沢市
    • Year and Date
      2011-09-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 心筋・線維芽細胞間ギャップ結合コミュニケーションは心筋の興奮伝導を障害する2011

    • Author(s)
      足達哲也、田中秀央、姜艶、橋本恵美、高松哲郎
    • Organizer
      日本組織細胞化学会
    • Place of Presentation
      金沢市
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 虚血・再灌流障害心の収縮帯形成はβジストログリカンの脱落によって生じる2011

    • Author(s)
      田中秀央、足達哲也、姜艶、橋本恵美、高松哲郎
    • Organizer
      日本組織細胞化学会
    • Place of Presentation
      金沢市
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dissipation of beta-dystroglycan with resultant hypercontracture of sarcomeres is culprit for formation of contraction bands in rat injured hearts2011

    • Author(s)
      Tanaka H, Mani, H, Adachi T, Jiang Y, Takamatsu T
    • Organizer
      European Heart Congress 2011
    • Place of Presentation
      フランス共和国パリ市
    • Year and Date
      2011-08-30
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 心筋・線維芽細胞間ギャップ結合コミュニケーションは興奮伝導を障害する2011

    • Author(s)
      足達哲也、田中秀央、姜艶、橋本恵美、高松哲郎
    • Organizer
      日本病理学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-04-28
    • Related Report
      2011 Final Research Report
  • [Presentation] 虚血・再灌流障害心の収縮帯形成におけるコスタメアの役割2011

    • Author(s)
      田中秀央、万井広基、足達哲也、 姜艶、高松哲郎
    • Organizer
      日本病理学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-04-28
    • Related Report
      2011 Final Research Report
  • [Presentation] 心筋・線維芽細胞間ギャップ結合コミュニケーションは興奮伝導を障害する2011

    • Author(s)
      足達哲也、田中秀央、姜艶、橋本恵美、高松哲郎
    • Organizer
      日本病理学会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-04-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 虚血・再灌流障害心の収縮帯形成におけるコスタメアの役割2011

    • Author(s)
      田中秀央、万井広基、足達哲也、姜艶、高松哲郎
    • Organizer
      日本病理学会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-04-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 膜電位蛍光プローブによる心房内興奮伝播の光学的イメージング2010

    • Author(s)
      松山高明、田中秀央、足達哲也、姜艶、高松哲郎
    • Organizer
      第51回日本組織細胞力化学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 心筋の収縮帯形成における細胞接着・細胞骨格系の関与2010

    • Author(s)
      田中秀央、万井弘基、足達哲也、姜艶、高松哲郎
    • Organizer
      第99回日本病理学会総会
    • Place of Presentation
      東京
    • Year and Date
      2010-04-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 心筋梗塞協会組織における心筋細胞・線維芽細胞間ギャップ結合コミュニケーション2009

    • Author(s)
      田中秀央, 他
    • Organizer
      第50回日本組織細胞科学会学術集会
    • Place of Presentation
      大津
    • Year and Date
      2009-09-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 興奮伝導異常の高速イメージング2009

    • Author(s)
      田中秀央、中上拓男、高松哲郎
    • Organizer
      日本バイオイメージング学会第18回学術集会
    • Place of Presentation
      岡山
    • Year and Date
      2009-09-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Regional heterogeneity of connexin-43 coupling promotes susceptibility to reentrant arrhythmias in rat cultured myocvte monolayers2009

    • Author(s)
      Tanaka H, Nakagami T, Takamatsu T
    • Organizer
      36^<th> International Congress of Physiological Sciences
    • Place of Presentation
      京都
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 心筋カルシウム動態異常の不整脈源性2009

    • Author(s)
      田中秀央、高松哲郎
    • Organizer
      第26回日本心電学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      2009-07-02
    • Related Report
      2009 Annual Research Report
  • [Book] 疾患モデルマウス表現型解析指南2011心筋虚血2011

    • Author(s)
      田中秀央、足達哲也
    • Publisher
      中山書店
    • Related Report
      2011 Final Research Report
  • [Book] 組織細胞化学2011 光による生体分子イメージング技術2011

    • Author(s)
      田中秀央、高松哲郎
    • Publisher
      学際企画
    • Related Report
      2011 Final Research Report
  • [Book] 疾患モデルマウス表現型解析指南2011

    • Author(s)
      田中秀央、足達哲也
    • Total Pages
      7
    • Publisher
      中山書店
    • Related Report
      2011 Annual Research Report
  • [Book] 組織細胞化学2011

    • Author(s)
      田中秀央、高松哲郎
    • Total Pages
      7
    • Publisher
      学際企画
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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