Ductular metaplasia of hepatocytes : its demonstration in vivo and the study of the mechanism
| Project/Area Number |
21590426
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ENOMOTO Katsuhiko 秋田大学, 医学部, 教授 (20151988)
OMORI Yasufumi 秋田大学, 医学部, 講師 (90323138)
YOSHIOKA Toshiaki 秋田大学, 医学部, 助教 (80302264)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 肝細胞 / 胆管上皮細胞 / 慢性肝傷害 / 細胆管反応 / 分化転換 / JNK-c-Jun経路 / 肝幹細胞 / JNL-c-Jun経路 / 肝細胞標識追跡系 / 慢性肝障害 / JNK-c-lun経路 |
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| Research Abstract |
To demonstrate transdifferentiation of hepatocytes to bile dutular cells in vivo, we established a hepatocyte-lineage tracing system, in which hepatocytes from Alb^<Cre/WT>ROSA26R (β-galactosidase-positive) were transplanted into the livers of C56BL/6J mice. In chronic liver injury induced by CCl_4 or DDC in these mice, we often found β-galactosidase-positive and cytokeratin 19-positive ductules, demonstrating that ductular transdifferentiation actually occurs in vivo. In addition, we found that hepatocyes from MKK7 KO mice (MKK7^<fl/fl>Alb^<Cre/WT>) showed less prominent branching morphogenesis as compared with those from control mice (MKK7^<fl/+>Alb^<Cre/WT>).
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Significance of integrin αvβ5and erbB3 in enhanced cell migration andliver metastasis of colon carcinomasstimulated by hepatocyte-derivedheregulin2010
Author(s)
Yoshioka T, Nishikawa Y, Ito R, Kawamata M, Doi Y, YamamotoY, YoshidaM, Omori Y, Kotanagi H, Masuko T, Enomoto K
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Journal Title
Cancer Sci
Volume: 101
Issue: 9
Pages: 2011-2018
DOI
Related Report
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[Journal Article] Mast cellleukemia with rapidly progressing portalhypertension2009
Author(s)
Yoshida M, Nishikawa Y, Yamamoto Y, Doi Y, Tokairin T, Yoshioka T, Omori Y, Watanabe A, Takahashi N, Yoshioka T, Miura I, Sawada K, Enomoto K
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Journal Title
Pathol Int
Volume: 59
Issue: 11
Pages: 817-822
DOI
NAID
Related Report
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