The gene expression profiling of lesional skin mast cells collected by laser capture microdissection

• Project/Area Number: 21590431
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kinki University
• Principal Investigator: ITO Akihiko 近畿大学, 医学部, 教授 (80273647)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アトピー性皮膚炎 / 接着分子 / 炎症 / mRNA / CADM1 / 神経-マスト細胞相互作用 / アドピー性皮膚炎 / 異染色性 / DNAマイクロアレイ / RNA増幅

Research Abstract

Atopic dermatitis(AD)-like lesional and non-lesional mast cells were separately collected by laser capture microdissection. Increased expression of CADM1 in lesional mast cells appeared to be a cause of enhanced sensory nerve-mast cell interaction in AD.

Research Products

• [Journal Article] Upregulation of Notch2 and Six1 is associated with Progression of Early-Stage Lung Adenocarcinoma and a More Aggressive Phenotype at Advanced (2012)
  • Author(s): Mimae, et al
  • Journal Title: Clin Cancer Res Volume: 18 Pages: 945-955
  • Peer Reviewed
• [Journal Article] Tumor suppressor cell adhesion molecule 1 (CADM1) is cleaved by A disintegrin and metalloprotease 10 (ADAM10) and subsequently cleaved by (2012)
  • Author(s): Nagara, et al
  • Journal Title: Biochem Biophys Res Commun Volume: 417 Pages: 462-467
  • Peer Reviewed
• [Journal Article] Unc93B1 Restricts Systemic Lethal Inflammation by Orchestrating Toll-like Receptor 7 and 9 Trafficking (2011)
  • Author(s): Fukui R, Saitoh S, Kanno A, Onji M, Shibata T, Ito A, Onji M, Matsumoto M, Akira S, Yoshida N, and Miyake K
  • Journal Title: Immunity Volume: 35 Pages: 69-81
  • Peer Reviewed
• [Journal Article] Neonatal lethality in knockout mice expressing the kinase-dead form of the gefinitib target GAK is caused by pulmonary dysfunction (2011)
  • Author(s): Tabara H, Naito Y, Ito A, Katsuma A, Sakurai MA, Ohno S, Shimizu H, Yabuta N, and Nojima H
  • Journal Title: Plos One Volume: 6
  • Peer Reviewed
• [Journal Article] Noncontact estimation of intercellular breaking force using a femtosecond laser impulse quantified by atomic force microscopy (2011)
  • Author(s): Hosokawa Y, Hagiyama M, Iino T, Murakami Y, and Ito A
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108 Pages: 1777-1782
  • Peer Reviewed
• [Journal Article] Enhanced nerve-mast cell interaction by a neuronal short isoform of cell adhesion molecule-1, CADM1 (2011)
  • Author(s): Hagiyama M, Furuno T, Hosokawa Y, Iino T, Ito T, Inoue T, Nakanishi M, Murakami Y, and Ito A
  • Journal Title: J Immunol Volume: 186 Pages: 5983-5992
  • Peer Reviewed
• [Journal Article] Aberrations of a cell adhesion molecule CADM4 in renal clear cell carcinoma (2011)
  • Author(s): Nagata M, Sakurai-Yageta M, Yamada D, Ito A, Fukuhara H, Kume H, Morikawa T, Fukayama M, Homma Y, and Murakami Y
  • Journal Title: Int J Cancer Volume: 130 Pages: 1329-1337
  • Peer Reviewed
• [Journal Article] Exercise increases neural stem cell proliferation surrounding the area of damage following rat traumatic brain injury (2011)
  • Author(s): Itoh T, Imano M, Nishida S, Tsubaki M, Hashimoto S, Ito A, and Satou T
  • Journal Title: J Neural Transm Volume: 118 Pages: 193-202
  • Peer Reviewed
• [Journal Article] Exercise inhibits neuronal apoptosis and improves cerebral function following rat traumatic brain injury (2011)
  • Author(s): Itoh T, Imano M, Nishida S, Tsubaki M, Hashimoto S, Ito A, and Satou T
  • Journal Title: J Neural Transm Volume: 118 Pages: 1263-1272
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of the CADM1 gene by retinoic acid during the neural differentiation of murine embryonal carcinoma P19 cells (2011)
  • Author(s): Ito T, Williams-Nate Y, Iwai M, Tsuboi Y, Hagiyama M, Ito A, Sakurai-Yageta M, and Murakami Y
  • Journal Title: Genes Cells Volume: 16 Pages: 791-802
  • Peer Reviewed
• [Journal Article] Aberrant expression of tumor suppressors CADM1 and 4.1B, in invasive lesions of primary breast cancer (2011)
  • Author(s): Takahashi Y, Iwai M, Kawai T, Arakawa A, Ito T, Sakurai-Yageta M, Ito A, Goto A, Saito M, Kasumi F, and Murakami Y
  • Journal Title: Breast Cancer Volume: (in press)
  • NAID: 10031170651
  • Peer Reviewed
• [Journal Article] (-)-epigallocatechin-3-gallate protects against neuronal cell death and improves cerebral function after traumatic brain injury in rats (2011)
  • Author(s): Itoh T, Imano M, Nishida S, Tsubaki M, Hashimoto S, Ito A, and Satou T
  • Journal Title: Neuromol Med Volume: 13 Pages: 300-309
  • Peer Reviewed
• [Journal Article] Enhanced nerve-mast cell interaction by a neuronal short isoform of cell adhesion molecule-1 (2011)
  • Author(s): Hagiyama, et al
  • Journal Title: J Immunol Volume: 186 Pages: 5983-5992
  • Peer Reviewed
• [Journal Article] Noncontact estimation of intercellular breaking force using a femtosecond laser impulse quantified by atomic force microscopy (2011)
  • Author(s): Hosokawa, et al
  • Journal Title: Proc Natl Sci U S A Volume: 108 Pages: 1777-1782
  • Peer Reviewed
• [Journal Article] Unc93B1 restricts systemic lethal inflammation by orchestrating Toll-like receptor 7 and 9 trafficking (2011)
  • Author(s): Fukui, et al
  • Journal Title: Immunity Volume: 35 Pages: 69-81
  • Peer Reviewed
• [Journal Article] CXC chemokine receptor 4 expressed in T cells plays an important role in the development of collagen-induced arthritis. (2011)
  • Author(s): Chung, et al.
  • Journal Title: Arthritis Res Ther Volume: 108 Pages: 1777-1782
  • Peer Reviewed
• [Journal Article] CADM1 interacts with Tiam1 and promotes invasive phenotype of human T-cell leukemia virus type I(HTLV-I) transformed cells and adult T-cell leukemia(ATL) cells : possible involvement of CADM1 in pathogenesis of ATL (2010)
  • Author(s): Masuda M, Maruyama T, Ohta T, Ito A, Hayashi T, Tsukasaki K, Kamihira S, Yamaoka S, Hoshino H, Yoshida T, Watanabe T, Stanbridge EJ, and Murakami Y
  • Journal Title: J Biol Chem Volume: 285 Pages: 15511-15522
  • Peer Reviewed
• [Journal Article] Epigenetic silencing of a proapoptotic cell adhesion molecule, the immunoglobulin superfamily member IGSF4, by promoter CpG methylation protects Hodgkin lymphoma cells from apoptosis (2010)
  • Author(s): Murray PG, Fan Y, Davies G, Ying J, Geng H, Ng KM, Li H, Gao Z, Wei W, Bose S, Anderton J, Kapatai G, Reynolds G, Ito A, Marafioti T, Woodman CB, Ambinder R, and Tao Q
  • Journal Title: Am J Pathol Volume: 177 Pages: 1480-1490
  • Peer Reviewed
• [Journal Article] Advantage of absorbable suture material for pulmonary artery ligation (2010)
  • Author(s): Mimae T, Hirayasu T, Kimura KB, Ito A, Miyata Y, and Okada M
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 58 Pages: 511-515
  • NAID: 10027470487
  • Peer Reviewed
• [Journal Article] CXC chemokine receptor 4 expressed in T cells plays an important role in the development of collagen-induced arthritis (2010)
  • Author(s): Chung SH, Seki K, Choi BI, Kimura KB Ito A, Fujikado N, Saijo S, and Iwakura Y
  • Journal Title: Arthritis Res Ther Volume: 12
  • Peer Reviewed
• [Journal Article] CADM1 interacts with Tiaml and promotes invasive phenotype of human T-cell leukemiavirus type I (HTLV-I) transformed cells and adult T-cell leukemia (ATL) cells. (2010)
  • Author(s): Masuda, et al.
  • Journal Title: J Biol Chem Volume: 285 Pages: 15511-15522
  • Peer Reviewed
• [Journal Article] Tumor suppressor CADMI is involEpigenetic silencing of a proapoptotic cell adhesion molecule, the immunoglobulin superfamily member IGSF4, by promoter CpG methylation protects Hodgkin lymphoma cells from apoptosis. (2010)
  • Author(s): Murray, et al.
  • Journal Title: Am J Pathol Volume: 177 Pages: 1480-1490
  • Peer Reviewed
• [Journal Article] Noncontact estimation of intercellular breaking force using a femtosecond laser impulse quantified by atomic force microscopy. (2010)
  • Author(s): Hosokawa, et al.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 12
  • Peer Reviewed
• [Journal Article] Advantage of absorbable suture material for pulmonary artery ligation. (2010)
  • Author(s): Mimae, et al.
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 58 Pages: 511-515
  • Peer Reviewed
• [Journal Article] Alternative lengthening of telomeres frequently occurs in mismatch repair system-deficient gastric carcinoma (2009)
  • Author(s): Omori Y, Nakayama F, Li D, Kanemitsu K, Semba S, Ito A, and Yokozaki H
  • Journal Title: Cancer Sci Volume: 100 Pages: 413-418
  • NAID: 10025143119
  • Peer Reviewed
• [Journal Article] Expression of a soluble isoform of cell adhesion molecule 1 in the brain and its involvement in directional neurite outgrowth (2009)
  • Author(s): Hagiyama M, Ichiyanagi N, Kimura KB, Murakami Y, and Ito A
  • Journal Title: Am J Pathol Volume: 174 Pages: 2278-2289
  • Peer Reviewed
• [Journal Article] Tumor suppressor CADM1 is involved in epithelial cell structure (2009)
  • Author(s): Sakurai-Yageta M, Masuda M, Tsuboi Y, Ito A, and Murakami Y
  • Journal Title: Biochem Biophys Res Commun Volume: 390 Pages: 977-982
  • Peer Reviewed
• [Journal Article] Expression of a soluble isoform of cell adhesion molecule 1 in the brain and its involvement in directional neurite outgrowth. (2009)
  • Author(s): Hagiyama, et al.
  • Journal Title: Am J Pathol Volume: 174 Pages: 2278-2289
  • Peer Reviewed
• [Journal Article] Tumor suppressor CADM1 is involved in epithelial cell structure. (2009)
  • Author(s): Sakurai, et al.
  • Journal Title: Biochem Biophys Res Commun Volume: 390 Pages: 977-982
  • Peer Reviewed
• [Presentation] 組織構築を再現した培養形における細胞間接着の力学的解析:フェムト秒レーザーの応用 (2011)
  • Author(s): 萩山, ら
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋(名古屋国際会議場)
  • Year and Date: 2011-10-03
• [Presentation] A kinetic analysis of nerve-mast cell adhesion using femtosecond laser (2011)
  • Author(s): Hagiyama, et al
  • Organizer: JSICR-MMCB2011
  • Place of Presentation: 大阪(ANAゲートタワーホテル)
  • Year and Date: 2011-05-25
• [Presentation] CADM1のスプライシングによる神経-マスト細胞相互作用の発生時期特異的な制御 (2011)
  • Author(s): 萩山, ら
  • Organizer: 日本病理学会
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 2011-04-30
• [Presentation] 組織構築を再現した培養形における細胞間接着の力学的解析:フェムト秒レーザーの応用 (2011)
  • Author(s): 伊藤彰彦
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] A kinetic analysis of nerve-mast cell adhesion using femtosecond laser (2011)
  • Author(s): Ito A
  • Organizer: The joint international meeting of the 76th annual meeting of the Japanese society for interferon and cytokine research, the 19th international symposium of macrophage molecular and cell biology 2011
  • Place of Presentation: Osaka
• [Presentation] CADM1のスプライシングによる神経-マスト細胞相互作用の発生時期特異的な制御 (2011)
  • Author(s): 伊藤彰彦
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
• [Presentation] フェムト秒レーザーの衝撃力を用いた細胞間接着力測定法の開発 (2010)
  • Author(s): 伊藤彰彦, 他
  • Organizer: 日本病理学会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-28
• [Presentation] フェムト秒レーザーによる白血球-血管内皮細胞間接着力の評価 (2010)
  • Author(s): 伊藤彰彦
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] フェムト秒レーザーによる神経-マスト細胞間接着力の測定 (2009)
  • Author(s): 伊藤彰彦, 他
  • Organizer: 日本病理学会
  • Place of Presentation: 京都
  • Year and Date: 2009-05-02
• [Presentation] マウス脳における接着分子CADM1分泌型アイソフォームの発現同定と神経伸長ガイダンスへの関与 (2009)
  • Author(s): 伊藤彰彦
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜