Study of Ret proapoptotic activity and its relation with human diseases
Project/Area Number |
21590436
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Experimental pathology
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Research Institution | Nagoya University |
Principal Investigator |
ASAI Naoya 名古屋大学, 大学院・医学系研究科, 准教授 (80273233)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | Retチロシンキナーゼ / 依存性受容体 / アポトーシス / 遺伝子改変マウス / 神経発生 / 下垂体発生 / 転写因子 / チロシンキナーゼ |
Research Abstract |
Ret tyrosine kinasse is a member of“dependence receptor"which induces apoptosis in the absence of its ligands. Ret is cleaved at D707 and D1017 by caspase, and intracellular fragment between D707 and D1017 can induce cell death. To determine the importance of Ret proapoptotic activity in vivo, we generated Ret D707N mutant mice. D707N Homozygote showed hyperplasia of neural cells in enteric, sympathetic and parapympathetic neural systems. In addition, adenopituitary lobe in pituitary gland was hyperplastic in D707N mice. To study the molecular mechanism of Ret proapoptotic activity, we examined the biological function of Ret fragment. Interestingly, Ret fragment is localized in the cell nucleus, and Ret fragment shows suppressive transcriptional activity.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Partial impairment of c-Ret at tyrosine1062 accelerates age-related hearing loss in mice2012
Author(s)
Ohgami N, Ida-Eto M, Sakashita N, Sone M, Nakashima T, Tabuchi K, Hoshino T, Shimada A, Tsuzuki T, Yamamoto M, Sobue G, Jijiwa M, Asai N, Hara A, Takahashi M, Kato M.
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Journal Title
Neurobiol Aging
Volume: 33(3)
Related Report
Peer Reviewed
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[Journal Article] Loss of Sprouty2 partially rescues renalhy poplasia and stomach hypoganglionosisbut not intestinal aganglionosis in RetY1062F mutant mice2011
Author(s)
Miyamoto R, Jijiwa M, Asai M, Kawai K, Ishida-Takagishi M, Mii S, Asai N, Enomoto A, Murakumo Y, Yoshimura A, Takahashi M.
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Journal Title
Dev Biol
Volume: 349(2)
Pages: 160-8
Related Report
Peer Reviewed
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[Journal Article] c-Ret-mediated hearing loss in mice with Hirschsprung disease2010
Author(s)
Ohgami N, Ida-Eto M, Shimotake T, Sakashita N, Sone M, Nakashima T, TabuchiK, Hoshino T, Shimada A, Tsuzuki T, Yamamoto M, Sobue G, Jijiwa M, Asai N, Hara A, Takahashi M, Kato M.
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Journal Title
Proc Natl Acad Sci U S A
Volume: 107(29)
Pages: 13051-6
Related Report
Peer Reviewed
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[Journal Article] Analysis of DOK-6 function in downstream signaling of RET in human neuroblastoma cells2010
Author(s)
Kurotsuchi A, Murakumo Y, Jijiwa M, Kurokawa K, Itoh Y, Kodama Y, Kato T, Enomoto A, Asai N, Terasaki H, Takahashi M.
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Journal Title
Cancer Sci
Volume: 101(5)
Pages: 1147-55
NAID
Related Report
Peer Reviewed
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[Journal Article] Etv4 and Etv5 are required downstream of GDNF and Ret for kidney branching morphogenesis2009
Author(s)
Lu BC, Cebrian C, Chi X, Kuure S, Kuo R, Bates CM, Arber S, Hassell J, MacNeil L, Hoshi M, Jain S, Asai N, Takahashi M, Schmidt-Ott KM, Barasch J, D' Agati V, Costantini F.
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Journal Title
Nat Genet
Volume: 41(12)
Pages: 1295-302
Related Report
Peer Reviewed
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[Journal Article] Ret-dependent cell rearrangements in the Wolffian duct epithelium initiate ureteric bud morphogenesis2009
Author(s)
Chi X, Michos O, Shakya R, Riccio P, Enomoto H, Licht JD, Asai N, Takahashi M, Ohgami N, Kato M, Mendelsohn C, Costantini F.
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Journal Title
Dev Cell
Volume: 17(2)
Pages: 199-209
Related Report
Peer Reviewed
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