Regulation of cell survival and malignant tumor formation by Drs/GADD34

• Project/Area Number: 21590437
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: INOUE Hirokazu 滋賀医科大学, 医学部, 准教授 (30176440)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 癌抑制遺伝子 / ストレス応答 / アポトーシス / オートファジー / 生体防御 / mTOR / エネルギー代謝 / ウイルス感染

Research Abstract

We investigated the role of Drs/GADD34 in cell responses under various stress conditions by using gene knockout cells and found that Drs plays critical roles for the control of cell responses in energy deprivation, hypoxia, energy metabolism and viral infection. Drs binds to GADD34 to regulate mTOR pathway via TSC1/2 phosphorylation control

Research Products

• [Journal Article] Suppression of viral replication by drs tumor suppressor via mTOR dependent pathway (2012)
  • Author(s): Tambe, Y., Okuyama, N., Nakagawa, T., Muramoto, A., Hasebe, M., Chano, T., Inoue, H.
  • Journal Title: Cancer Lett Volume: 314 Pages: 82-91
  • Peer Reviewed
• [Journal Article] Suppression of viral replication by drs tumor suppressor via mTOR dependent pathway (2012)
  • Author(s): Tambe, Y., et al
  • Journal Title: Cancer Letters Volume: 314 Issue: 1 Pages: 82-91
  • DOI: 10.1016/j.canlet.2011.09.015
  • Peer Reviewed
• [Journal Article] RB1CC1 activates the p16 promoter through the interaction with hSNF5 (2011)
  • Author(s): Ochi, Y., Chano, T., Ikebuchi, K., Inoue, H., Isono, T., Arai, A., Tameno, H., Shimada, T., Hisa, Y., Okabe, H.
  • Journal Title: Oncol. Rep Volume: 26 Pages: 805-812
  • Peer Reviewed
• [Journal Article] Opposite regulation of epithelial-to-mesenchymal transition and cell invasiveness by periostin between prostate and bladder cancer cells (2011)
  • Author(s): Kim, C. J., Sakamoto, K., Tambe, Y., Inoue, H.
  • Journal Title: Int. J. Oncol Volume: 38 Pages: 1750-1766
  • Peer Reviewed
• [Journal Article] Opposite regulation of epitnelial-to mesenchymal transition and cell invasiveness by periostin between prostate and bladder cance cells (2011)
  • Author(s): Kim, C.J., et al
  • Journal Title: Int.J.One. Volume: 38 Pages: 1759-1766
  • DOI: 10.3892/ijo.2011.997
  • Peer Reviewed
• [Journal Article] RBCC1 activates the p16 promoter through the interaction with hSNF5 (2011)
  • Author(s): Ochi, Y., et al
  • Journal Title: Oncol.Rep. Volume: 26 Pages: 805-812
  • DOI: 10.3892/or.2011.1329
  • Peer Reviewed
• [Journal Article] Opposite regulation of epithelial-to-mesenchymal transition and cell invasiveness by periostin between prostate and bladder cancer cells. (2011)
  • Author(s): Kim, C.J., Sakamoto, K., Tambe, Y., Inoue, H.
  • Journal Title: Int.J.Oncol. Volume: 38 Pages: 1759-1766
  • Peer Reviewed
• [Journal Article] RBCC1 together with RB1 and p53 predicts long-term survival in Japanese breast cancer patients (2010)
  • Author(s): Chano, T., Ikebuchi, K., Tomita, Y., Jin, Y., Inaji, H., Ishitobi, M., Teramoto, K., Ochi, Y., Tameno, H., Nishimura, I., Minami, K., Inoue, H., Isono, T., Saitoh, M., Shimada, T., Hisa, Y., Okabe, H.
  • Journal Title: PLos ONE Volume: 5
  • Peer Reviewed
• [Journal Article] RBCC1 activates RB1 pathway and inhibits proliferation and cologenic survival in human cancer (2010)
  • Author(s): Chano, T., Ikebuchi, K., Ochi, Y., Tameno, H., Tomita, Y., Jin, Y., Inaji, H., Ishitobi, M., Teramoto, K., Nishimura, I., Minami, K., Inoue, H., Isono, T., Saitoh, M., Shimada, T., Hisa, Y., Okabe, H.
  • Journal Title: PLos ONE Volume: 5
  • Peer Reviewed
• [Journal Article] RBCC1 activates RB1 pathway and inhibits proliferation and cologenic survival in human cancer. (2010)
  • Author(s): Chano, T., Ikebuchi, K., Ochi, Y., Tameno, H., Tomita, Y., Jin, Y., Inaji, H., Ishitobi, M., Teramoto, K., Nishimura, I., Minami, K., Inoue, H., Isono, T., Saitoh, M., Shimada, T., Hisa, Y., Okabe, H.
  • Journal Title: PLos ONE Volume: 5
  • Peer Reviewed
• [Journal Article] RBCC1 together with RB1 and p53 predicts long-term survival in Japanese breast cancer patients. (2010)
  • Author(s): Chano, T., Ikebuchi, K., Tomita, Y., Jin, Y., Inaji, H., Ishitobi, M., Teramoto, K., Ochi, Y., Tameno, H., Nishimura, I., Minami, K., Inoue, H., Isono, T., Saitoh, M., Shimada, T., Hisa, Y., Okabe, H.
  • Journal Title: PLos ONE Volume: 5
  • Peer Reviewed
• [Journal Article] The Drs tumor suppressor is involved in the maturation process of autophagy induced by low serum (2009)
  • Author(s): Tambe, Y., Yamamoto, A., Isono, T., Chano, T., Fukuda, M. and Inoue, H.
  • Journal Title: Cancer Lett Volume: 283 Pages: 74-83
  • Peer Reviewed
• [Journal Article] Suppression of cell invasiveness by periostin via TAB1/TAK1 (2009)
  • Author(s): Isono, T., Kim, C. J., Ando, Y., Sakurai, H., Okada, Y., and Inoue, H.
  • Journal Title: Int. J. Oncol Volume: 35 Pages: 425-432
  • Peer Reviewed
• [Journal Article] Cyclin D1b variant promotes cell invasiveness independent of binding to CDK in human bladder cancer cells (2009)
  • Author(s): Kim, C. J., Nishi, K., Isono, T., Okuyama, Y., Tambe, Y., Okada, Y., and Inoue, H.
  • Journal Title: Mol. Carcinog Volume: 48 Pages: 953-964
  • Peer Reviewed
• [Journal Article] Downregulation of drs tumor suppressor gene in highly malignant human pulmonary neuroendocrine tumors (2009)
  • Author(s): Shimakage, M., Kodama, K., Kawahara, K., Kim, C. J., Ikeda, Y., Yutsudo, M., Inoue, H.
  • Journal Title: Oncol. Rep Volume: 21 Pages: 1367-1372
  • Peer Reviewed
• [Journal Article] Cyclin D1 downregulation is important for permanent cell cycle exit and initiation of differentiation induced by anchorage-deprivation in human keratinocytes (2009)
  • Author(s): Nishi, K., Inoue, H., Schnier, J. B., and Rice, R. H.
  • Journal Title: J. Cell. Biohem Volume: 106 Pages: 63-72
  • Peer Reviewed
• [Journal Article] Downregulation of drs tumor suppressor gene in highly malignant human pulmonary neuroendocrine tumors (2009)
  • Author(s): Shimakage, M., Kodama, K., Kawahara, K., Kim, C.J., Ikeda, Y., Yutsudo, M., Inoue, H.
  • Journal Title: Oncology Reports 30 Pages: 1343-1348
  • Peer Reviewed
• [Journal Article] Cyclin D1b variant prpmotes cell invasiveness independent of binding to CDK in human bladder cancer cells (2009)
  • Author(s): Kim, C.J., Nishi, K., Isono, T., Okuyama, Y., Tambe, Y., Okada, Y., Inoue, H.
  • Journal Title: Molecular Carcinogenesis 48 Pages: 953-964
  • Peer Reviewed
• [Journal Article] The Drs tumor suppressor is involved in the maturation process of autophagy induced by low serum (2009)
  • Author(s): Tambe, Y., Yamamoto, A., Isono, T., Chano, T., Fukuda, M., Inoue, H.
  • Journal Title: Cancer Letters 283 Pages: 74-83
  • Peer Reviewed
• [Journal Article] Suppression of cell invasiveness by periostin via TAB1/TAK1 (2009)
  • Author(s): Isono, T., Kim, C.J., Ando, Y., Sakurai, H., Okada, Y., Inoue, H.
  • Journal Title: Int.J.Oncol. 35 Pages: 425-432
  • Peer Reviewed
• [Presentation] Roll of the drs tumor suppressor in regulation of energy metabolism (2011)
  • Author(s): Yukihiro Tarabe, Hirokazu inoue
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-03
• [Presentation] Roll of the drs tumor suppressor in regulation of energy metabolism (2011)
  • Author(s): Yukihiro Tambe, Hirokazu Inoue
  • Organizer: 第70回日本癌学会総会
• [Presentation] Role of cyclin D1b variant in rectal tumorigenesis (2011)
  • Author(s): Chul Jang Kim, Yukihiro Tambe, Takahiro Isono, Ken-ichi Mukaisho, Hiroyuki Sugihara, Gen Kondoh, Hirokazu Inoue
  • Organizer: 第70回日本癌学会総会
• [Presentation] Cell-type dependent regulation of EMT and cell invasiveness of human cancer cells by periostin (2010)
  • Author(s): Hirokazu Inoue, Kanami Sakamoto, Yukihiro Tambe, Chul Jang Kim
  • Organizer: 第33回日本分子生物学会年会
• [Presentation] 癌抑制遺伝子drsによるウイルス増殖抑制作用 (2010)
  • Author(s): 旦部幸博、奥山直美、中川達也、井上寛一
  • Organizer: 第33回日本分子生物学会年会
• [Presentation] The drs tumor suppressor is involved in the suppression of viral replication via p38-RSK1-S6 dependent pathway (2010)
  • Author(s): Yukihiro Tambe, Tokuhiro Chano, Hirokazu Inoue
  • Organizer: 第69回日本癌学会総会
• [Presentation] Cell-type dependent regulation of EMT and cell invasiveness of cancer cells by periostin (2010)
  • Author(s): Chul Jang Kim, Yukihiro Tambe, Hirokazu Inoue
  • Organizer: 第69回日本癌学会総会
• [Presentation] Activity of novel RBCC1 molecular cascade involving RB1 and p53 correlates with prognosis of breast cancers (2009)
  • Author(s): Tokuhiro Chano, Kaichiro Ikebuchi, Yasuhiko Tomita, Hitosuke Tameno, Yasuko Ochi, Kahori Minami, Hirokazu Inoue, Takahiro Isono, Masao Saitoh, Hidetoshi Okabe
  • Organizer: 第68回日本癌学会総会
• [Presentation] Functional analysis of variant cyclin D1b on the enhancement of cell invasiveness in bladder cancer cells (2009)
  • Author(s): Chul Jang Kim, Takahiro Isono, Yukihiro Tambe, Yusaku Okada, Hirokazu Inoue
  • Organizer: 第68回日本癌学会総会
• [Presentation] Suppression of viral replication by the drs tumor suppressor (2009)
  • Author(s): Yukihiro Tambe, Hirokazu Inoue
  • Organizer: 第68回日本癌学会総会
• [Presentation] Role of the drs tumor suppressorgene in cell survival under energy depletion (2009)
  • Author(s): Hirokazu Inoue, Tokuhiro Chano, Yukihiro Tambe
  • Organizer: 第68回日本癌学会総会
• [Presentation] Role of the drs tumor suppressor gene in cell survival under energy depletion (2009)
  • Author(s): Hirokazu Inoue, Tokuhiro Chano, Yukihiro Tambe
  • Organizer: 第68回日本癌学会総会
  • Place of Presentation: 横浜
• [Remarks]
  • URL: http://www.shiga-med.ac.jp/~hg.micro/