Study of lung epithelial and lung cancer stem cells of the lung-specific gene-targeted mice ; analysis by lung epithelial sphere assay
| Project/Area Number |
21590440
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kumamoto University |
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Principal Investigator |
ITO Takaaki 熊本大学, 大学院・生命科学研究部, 教授 (70168392)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Tetuhiro 熊本大学, 大学院・生命科学研究部, 助教 (70359818)
UDAKA Naoko 熊本大学, 大学院・生命科学研究部, 助教 (90285106)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 遺伝子改変マウス / EGFR / Hes1 / 肺 / 幹細胞 / Sca1 / epithelial sphere / 肺上皮細胞 / スフェア形成 / EGFR変異 / sca1陽性細胞 / 器官培養 / Hes1欠損 / マウス / 肺上皮幹細胞 / Scal陽性細胞 / Hesl欠損 / scal陽性肺上皮細胞 / 増殖因子 |
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| Research Abstract |
Lung epithelial sphere(LES) formation assay seems a good experimental method monitoring lung epithelial stem cells, and sca1-positive cells contain many lung epithelial stem cells. LESs were formed from mouse fetal epithelial cells, which lack sca1-positive cells. The lung epithelial cells having human mutant EGFR gene have high proliferative activity. In these cells, occurrence of sca1-positive cells does no increase, but LEC formation was mildly increased. In addition, significance of stem-cell related molecules was studied using surgically resected samples and cell lines.
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Report
(4 results)
Research Products
(13 results)
