Analysis of immunosuppression mechanism by sialic acids on cancer cells, and a feasible study to control immune reaction using sialylated carbohydrates.

• Project/Area Number: 21590454
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: IKEHARA Yuzuri 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, 研究チーム長 (10311440)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 炎症 / 糖鎖 / がん / 免疫制御 / 免疫応答 / シアル酸

Research Abstract

This study was conducted to increase knowledge about immune evasion by cancer cells, focusing on appearing sialic acids(Sialyl Tn antigen) with development and progression of cancer. Using xenotransplantation and syngenic transplantation animal models, and sialic acid coated liposome, we demonstrated that sialic acids had regulatory function to suppress tumor immunity. Additionally, we established methods to generate a series of immortalized cells that are available to investigate further mechanism of immune evasion.

Research Products

• [Journal Article] Establishment and characterization of novel gastric signet-ring cell and non signet-ring cell poorly differentiated adenocarcinoma cell lines with low and high malignant potential (2012)
  • Author(s): Murakami H, Nakanishi H, Tanaka H, Ito S, Misawa K, Ito Y, Ikehara Y, Kondo E, Kodera Y
  • Journal Title: Gastric Cancer
  • Peer Reviewed
• [Journal Article] PCR Detection of Babesia ovata from Cattle Reared in Japan and Clinical Significance of Co-Infection with Theileria orientalis (2012)
  • Author(s): Sivakumar T, Tagawa M, Yoshinari T, Ybanez AP, Igarashi I, Ikehara Y, Hata H, Kondo S, Matsumoto K, Inokuma H, Yokoyama N
  • Journal Title: J Clin Microbiol
  • Peer Reviewed
• [Journal Article] Enhancement of metastatic ability by ectopic expression of ST6GalNAcI on a gastric cancer cell line in a mouse model (2012)
  • Author(s): Ozaki H, Matsuzaki H, Ando H, Kaji H, Nakanishi H, Ikehara Y, Narimatsu H
  • Journal Title: Clin Exp Metastasis Volume: 29(3) Pages: 229-238
  • Peer Reviewed
• [Journal Article] Different levels of Sialyl-Tn Antigen expressed on MUC16 in Endometriosis and Ovarian Cancer Patients (2012)
  • Author(s): Akita K, Yoshida S, Ikehara Y, Shirakawa S, Toda M, Inoue M, Kitawaki J, Nakanishi H, Narimatsu H, Nakada H
  • Journal Title: Int J Gynecol Cancer
  • Peer Reviewed
• [Journal Article] Characterization of Splenic Cells during the Early Phase of Infection with Neuropathogenic Mouse Hepatitis Virus (2011)
  • Author(s): Kashiwazaki H, Taguchi F, Ikehara Y, Watanabe R
  • Journal Title: Jpn J Infect Dis Volume: 64(3) Pages: 256-259
  • NAID: 40018820945
  • Peer Reviewed
• [Journal Article] Single-walled carbon nanohorns as drug carriers : adsorption of prednisolone and anti-inflammatory effects on arthritis (2011)
  • Author(s): Nakamura M., Tsuchida K., 他6名
  • Journal Title: Nanotechnology Volume: 22巻 Issue: 46 Pages: 465102-465102
  • DOI: 10.1088/0957-4484/22/46/465102
  • Peer Reviewed
• [Journal Article] Different levels of Sialyl-Tn Antigen expressed on MUC16 in Endometriosis and Ovarian Cancer Patients (2011)
  • Author(s): Akita K, Yoshida S, Ikehara Y, Shirakawa S, Toda M, Inoue M, Kitawaki J, Nakanishi H, Narimatsu H, Nakada H
  • Journal Title: Int J Gynecol Cancer Volume: Feb 24(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Irradiation experiments on a mouse using a mild-plasma generator for medical applications (2010)
  • Author(s): SAKAKITA H. and IKEHARA Y
  • Journal Title: Plasma and Fusion Research Volume: S2117 Pages: 1-4
  • NAID: 130000669107
  • Peer Reviewed
• [Journal Article] Targeted delivery of oligomannose-coated liposome to the omental micrometastasis by peritoneal macrophages from patients with gastric cancer (2010)
  • Author(s): Matsui M., Shimizu Y., Kodera Y., Kondo, K. Ikehara Y., and Nakanishi H
  • Journal Title: Cancer Sci Volume: 101(7) Pages: 1670-7
  • NAID: 10026594802
  • Peer Reviewed
• [Journal Article] Recent advancements in cytotoxic T lymphocyte generation methods using carbohydrate coated liposomes (2010)
  • Author(s): Ikehara Y., Yamaguchi T., Yamanaka M.
  • Journal Title: J. Biomedicine and Biotechnology Volume: 242539 Pages: 1-8
  • DOI: 10.1155/2010/242539
  • Peer Reviewed
• [Journal Article] Irradiation experiments on a mouse using a mild-plasma generator for medical applications. (2010)
  • Author(s): SAKAKITA H., IKEHARA Y.
  • Journal Title: Plasma and Fusion Research Volume: S2117 Pages: 1-4
  • NAID: 130000669107
  • Peer Reviewed
• [Journal Article] Targeted delivery of oligomannose-coated liposome to the omental micrometastasis by peritoneal macrophages from patients with gastric cancer. (2010)
  • Author(s): Matsui M., Shimizu Y., Kodera Y., Kondo K., Ikehara Y., Nakanishi H.
  • Journal Title: Cancer Sci. Volume: 101(7) Pages: 1670-1677
  • NAID: 10026594802
  • Peer Reviewed
• [Journal Article] Recent advancements in cytotoxic T lymphocyte generation methods using carbohydrate coated liposomes. (2010)
  • Author(s): Ikehara Y., Yamaguchi T., Yamanaka M.
  • Journal Title: J.Biomedicine and Biotechnology Volume: 242539 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Immunization of oligomannose-coated liposome-entrapped NcGRA7 protects dams and offspring from Neospora caninum infection in mice (2009)
  • Author(s): Nishikawa Y, Zhang H, Ikehara Y, Kojima N, Xuan X, Yokoyama N
  • Journal Title: Clin Vaccine Immunol Volume: 16(6) Pages: 792-729
  • Peer Reviewed
• [Journal Article] Effect of oligomannose-coated liposome-based vaccine on rodent babesiosis (2009)
  • Author(s): Ishigame T., Takabatake N., Iseki H., Ota N., Koyama A., Igarashi I., Nishikawa Y., Ikehara Y., Kojima N., Yokoyama N
  • Journal Title: J. Protozoology Research Volume: 19(1) Pages: 10-23
  • Peer Reviewed
• [Journal Article] Strategy for Glycoproteomics : Identification of Glyco-Alteration Using Multiple Glycan Profiling Tool (2009)
  • Author(s): Ito H, Kuno A, Sawaki H, Sogabe M, Ozaki H, Tanaka Y, Mizokami M, Shoda JI, Angata T, Sato T, Hirabayashi J, Ikehara Y, Narimatsu H
  • Journal Title: J Proteome Res Volume: 8(3) Pages: 1358-67
  • Peer Reviewed
• [Journal Article] Identification of Glyco-Alteration Using Multiple Glycan Profiling Tool. (2009)
  • Author(s): Ito H, el al
  • Journal Title: J Proteome Res. 8(3) Pages: 1358-1367
  • Peer Reviewed
• [Journal Article] Immunization of oligomannose-coated liposome-entrapped NcGRA7 protects dams and offspring from Neospora caninum infection in mice. (2009)
  • Author(s): Nishikawa Y, et al
  • Journal Title: Clin Vaccine Immunol. 16(6) Pages: 792-729
  • Peer Reviewed
• [Journal Article] Effect of oligomannose-coated liposome-based vaccine on rodent babesiosis. (2009)
  • Author(s): Ishigame T.
  • Journal Title: J.Protozoology Research 19(1) Pages: 10-23
  • Peer Reviewed
• [Presentation] グライコプロテオミクス研究に必要な実験手技 (2011)
  • Author(s): 池原譲
  • Organizer: 第8回日本病理学会カンファレンス
  • Place of Presentation: ホテルブエナビスタ(長野県)
  • Year and Date: 2011-08-05
• [Presentation] 糖鎖分析技術を取り入れた組織細胞化学研究 (2011)
  • Author(s): 池原譲
  • Organizer: 組織細胞化学会講習会
  • Place of Presentation: 三鷹市公会堂(東京都)
  • Year and Date: 2011-08-04
• [Presentation] グライコプロテオミクスによる肝細胞がんに対する新規糖鎖マーカーの探索 (2011)
  • Author(s): 池原譲
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-04-30
• [Presentation] 糖鎖研究の進展に基づく癌の検出と治療戦略 (2010)
  • Author(s): 池原譲
  • Organizer: 日本組織細胞化学会
  • Place of Presentation: 秋葉原コンベショナルセンター(東京)
  • Year and Date: 2010-09-06
• [Presentation] 糖鎖研究の進展に基づく癌の検出と治療戦略 (2010)
  • Author(s): 池原譲
  • Organizer: 日本組織細胞化学会
  • Place of Presentation: 秋葉原コンベショナルセンター
  • Year and Date: 2010-09-06
• [Presentation] Cancer vaccine delivery system using oligomannose coated liposomes (2010)
  • Author(s): Ikehara Y.
  • Organizer: World Congress of Vaccine-2010
  • Place of Presentation: 北京国際会議場(Beijin)
  • Year and Date: 2010-03-25
• [Presentation] Cancer vaccine delivery system using oligomannose coated liposomes (2010)
  • Author(s): Yuzuru Ikehara
  • Organizer: World Congress of Vaccine -2010
  • Place of Presentation: Beijin
  • Year and Date: 2010-03-25
• [Presentation] 肝線維化の進展を測定できる新しい糖鎖バイオマーカーの開発とその有用性について (2009)
  • Author(s): 池原譲、成松久
  • Organizer: 日本分子腫瘍マーカー学
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-09-30
• [Presentation] 肝線維化の進展を測定できる新しい糖鎖バイオマーカーの開発とその有用性について (2009)
  • Author(s): 池原譲
  • Organizer: 日本分子腫瘍マーカー学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-09-30
• [Presentation] A novel drug delivery system. Carbohydrate recognition-based and controlled release system using intra-peritoneal macrophages as a cellular vehicle (2009)
  • Author(s): Ikehara Y., Niwa T., Ikehara S., Biao L., Ohashi N., Kobayashi T., Shimizu Y., Kojima N., Nakanishi H.
  • Organizer: AACR 100th Annual Meeting
  • Place of Presentation: Denver, CO(USA)
  • Year and Date: 2009-04-20
• [Presentation] Cancer vaccine delivery using oligomannose coated liposomes (2009)
  • Author(s): Y.Ikehara
  • Organizer: AACR 100th Annual Meeting 2009
  • Place of Presentation: Denver, CO
  • Year and Date: 2009-04-20
• [Book] 組織細胞化学2011、糖鎖分析技術を取り入れた組織細胞化学研究 (2011)
  • Author(s): 池原譲
  • Publisher: 日本組織細胞化学会出版
• [Book] 組織細胞化学2010、糖鎖・糖タンパク質の生物機能解明と医療応用 (2011)
  • Author(s): 池原譲
  • Publisher: 日本組織細胞化学会出版
• [Book] 組織細胞化学2010-組織細胞化学イメージングの基礎と最前線:生体内分子局在と機能を探る- (2010)
  • Author(s): 池原譲
  • Publisher: 日本組織細胞化学会編集
• [Remarks]
  • URL: http://unit.aist.go.jp/rcmg/rcmg-ga/ci/index.html