Roles of IFN-γ and MyD88 on dendritic cell-vaccine with T. gondii-HSP70
| Project/Area Number |
21590462
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Chiba University |
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Principal Investigator |
AOSAI Fumie 千葉大学, 大学院・医学研究院, 准教授 (80150316)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | トキソプラズマ / HSP70 / 樹状細胞 / 熱ショック蛋白70(HPS70) / IFN-γ / MyD88 / 樹状細胞(DC) / 細胞性防御免疫 / Th1/Th2/Th17 / 細胞傷害性T細胞(CTL) / interferon-gamma(IFN-γ) / myeloid differentiation factor 88(MvD88) / Th1/Th2/Th17分化誘導 / myeloid differentiation factor 88 (MyD88) / Th1 / Th2 / Th17分化誘導 |
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| Research Abstract |
Pathological findings in Toxoplasma gondii-infected host are regulated not only by differences in virulence or pathogenecity of T. gondii strains but also by genetic protective immunity of hosts. We have reported that FN-.deficient mice and MyD88 deficient mice succumbed within 7-10days. In the present study, these murine strains are used as immune-deficient mouse models and have analyzed the effects of T. gondii-HSP70 (T.g.HS70) gene vaccine targeting peripheral immature dendritic cells (DC). Firstly, DC from both FN-γ.and MyD88 deficient mice are capable to differentiate into mature DC by the stimulation with T.g.HS70 and successively induce Th1 polarization from Th0 of syngeneic naive WT mice. Secondary, T.g.HS70 gene vaccine succeeds to induce DC maturation and successive Th1 polarization of CD^4+ T cells in the draining lymph nodes (dLN) maximal at day 5 after the vaccine. Analyses of innate immunity in these events revealed that TLR4/MyD88 signal pathway is involved not only at acute phase but also at chronic phase of vaccine effects.
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Report
(4 results)
Research Products
(46 results)
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[Presentation] 遅延性再発性トキソプラズマ症の一例2011
Author(s)
神白麻衣子, 柿内聡志, 石藤智子, 古本朗嗣, 石田正之, 土橋佳子, 野呂瀬一美, 青才文江, 堤寛, 佐多徹太郎, 森本浩之輔, 有吉紅也
Organizer
第85回日本感染症学会総会抄録集170
Place of Presentation
東京
Year and Date
2011-04-21
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