| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
Pathological findings in Toxoplasma gondii-infected host are regulated not only by differences in virulence or pathogenecity of T. gondii strains but also by genetic protective immunity of hosts. We have reported that FN-.deficient mice and MyD88 deficient mice succumbed within 7-10days. In the present study, these murine strains are used as immune-deficient mouse models and have analyzed the effects of T. gondii-HSP70 (T.g.HS70) gene vaccine targeting peripheral immature dendritic cells (DC). Firstly, DC from both FN-γ.and MyD88 deficient mice are capable to differentiate into mature DC by the stimulation with T.g.HS70 and successively induce Th1 polarization from Th0 of syngeneic naive WT mice. Secondary, T.g.HS70 gene vaccine succeeds to induce DC maturation and successive Th1 polarization of CD^4+ T cells in the draining lymph nodes (dLN) maximal at day 5 after the vaccine. Analyses of innate immunity in these events revealed that TLR4/MyD88 signal pathway is involved not only at acute phase but also at chronic phase of vaccine effects.
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