Development of a new chemotherapy for cryptosporidiosis

• Project/Area Number: 21590467
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nagoya City University
• Principal Investigator: YABU Yoshisada 名古屋市立大学, 大学院・医学研究科, 講師 (70080083)
• Co-Investigator(Renkei-kenkyūsha): SAIMOTO Hiroyuki 鳥取大学, 大学院・工学研究科, 教授 (20186977)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: クリプトスポリジウム / アスコフラノン / 経口投与 / 腹腔内投与 / 静脈内投与 / 治療薬開発 / SCIDマウス / 免疫電顕

Research Abstract

Chemotherapeutic effect of ascofuranone, a new antibiotic, was determined using Cryptosporidium parvum-infected SCID mice. The drug was administrated to infected mice by oral, intraperitoneal, and intravenous route. A suspension of ascofuranone(100mg/kg) was given every 24 h for 6 consecutive days to infected mice and efficacy was evaluated by oocysts reduction in the feces of treated mice by fluorescent microscopically. Oocysts in the feces were over 2 x 10^5/g at the beginning of oral administration, the numbers of oocysts were gradually decreased to 8 x 10^3/g on day 6.After stopping the treatment, numbers of oocysts were increased to over 4 x 10^5/g on day 10.Almost same results were observed in intraperitoneally and intravenously administrations.

Research Products

• [Journal Article] Differential kinetic activities of glycerol kinase among African trypanosome species : phylogenetic and therapeutic implications (2011)
  • Author(s): Ohashi-Suzuki M, Yabu Y, Ohshima S, Nakamura K, Kido Y, Sakamoto K, Kita K, Ohta N, Suzuki T
  • Journal Title: J.Vet.Med.Sci. Volume: 73 Pages: 615-621
  • NAID: 130000444748
  • Peer Reviewed
• [Journal Article] Purification and kinetic characterization of recombinant alternative oxidase from Trypanosoma brucei brucei (2010)
  • Author(s): Kido Y., Sakamoto, K., Nakamura, K., Harada, M., Suzuki T., Yabu, Y., Saimoto, H., Yamakura F., Ohmori D., Moore A., Harada S., Kita K
  • Journal Title: Biochim. Biophys. Acta Volume: 1797巻 Pages: 443-450
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20056101
  • Peer Reviewed
• [Journal Article] Trypanosome alternative oxidase, a potential therapeutic target for sleeping sickness, is conserved among Trypanosoma brucei subspecies (2010)
  • Author(s): Nakamura K., Fujioka S., Fukumoto S., Inoue N., Sakamoto K., Hirata H., Kido Y., Yabu Y., Suzuki T., Watanabe Y., Saimoto H., Akiyama H., Kita k
  • Journal Title: Parasit. Int Volume: 59巻 Pages: 560-564
  • NAID: 10029826326
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20688188
  • Peer Reviewed
• [Journal Article] TbUNC119 and its binding protein complex are essential for propagation, motility, and morphogenesis of Trypanosoma brucei procyclic form cells (2010)
  • Author(s): Ohshima S., Ohashi-Suzuki M., Miura Y., Yabu Y., Okada N., Ohta N., Suzuki T
  • Journal Title: PLos ONE Volume: 5巻 Pages: 1-13
  • URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008729/?tool=pubmed
  • Peer Reviewed
• [Journal Article] Differential kinetic activities of glycerol kinase among African trypanosomes species : phylogenic and therapeutic implications (2010)
  • Author(s): Ohashi-Suzuki M., Yabu Y., Ohshima S., Nakamura K., Kido Y., Sakamoto K., Kita K., Ohta N., Suzuki T
  • Journal Title: J. Vet. Med. Sci. Volume: 73巻 Pages: 615-621
  • URL: https://www.jstage.jst.go.jp/article/jvms/73/5/73_10-0481/_article
  • Peer Reviewed
• [Journal Article] TbUNC119 and its binding protein complex are essential for propagation, motility and morphogenesis of Trypanosoma brucei procyclic form cells (2010)
  • Author(s): S.Ohshima, M.Ohashi-Suzuki, Y.Miura, Y.Yabu, N.Okada, N.Ohta, T.Suzuki
  • Journal Title: PLoS One Volume: 5 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Trypanosome alternative oxidase, a potential therapeutic target for sleeping sickness, is conserved among Trypanosoma brucei subspecies (2010)
  • Author(s): Nakamura K, Fujioka S, Fukumoto S, Inoue N, Sakamoto K, Hirata H, Kido Y, Yabu Y, Suzuki T, Watanabe Y, Saimoto H, Akiyama H, Kita K
  • Journal Title: Parasitology International Volume: 59 Pages: 560-564
  • NAID: 10029826326
  • Peer Reviewed
• [Journal Article] Purification and kinetic characterization of recombinant alternative oxidase from Trypanosoma brucei brucei (2010)
  • Author(s): Kido Y, Sakamoto K, Nakamura K, Harada M, Suzuki T, Yabu Y, Saimoto H, Yamakura F, Ohmori D, Moore A, Harada S, Kita K.
  • Journal Title: Biochim Biophys Acta 1797 Pages: 443-450
  • Peer Reviewed
• [Presentation] Cryptosporidium parvumシアン耐性酸化酵素(Alternative oxidase, AOX)の酵素学的解析 (2011)
  • Author(s): 原田倫世
  • Organizer: 第80回日本寄生虫学会
  • Place of Presentation: 東京慈恵会医科大学(東京)
  • Year and Date: 2011-07-18
• [Presentation] アフリカトリパノソーマ原虫の動き関連分子、TbUNC199の解析 (2011)
  • Author(s): 鈴木高史
  • Organizer: 第80回日本寄生虫学会
  • Place of Presentation: 東京慈恵会医科大学(東京)
  • Year and Date: 2011-07-18
• [Presentation] Cryptoaporidium parvumシアン耐性酸化酵素(Alternative oxidase, AOX)の酵素学的解析 (2011)
  • Author(s): 原田倫世、藤本陽子、松崎素道、城戸康年、坂元君年、籔義貞、中井裕、北潔
  • Organizer: 第80回日本寄生虫学会総会
  • Place of Presentation: 東京慈恵会医科大学本館(東京都)
  • Year and Date: 2011-07-18
• [Presentation] アフリカトリパノソーマ原虫の動き関連分子、TbUNC119の解析 (2011)
  • Author(s): 鈴木高史、大島茂、鈴木光子、籔義貞、太田伸生
  • Organizer: 第80回日本寄生虫学会総会
  • Place of Presentation: 東京慈恵会医科大学本館(東京都)
  • Year and Date: 2011-07-18
• [Presentation] Trichomonas foetusの市販培養液を用いた培養法の開発 (2010)
  • Author(s): 籔義貞、真田祥太郎、岡田則子
  • Organizer: 日本寄生虫学会西日本支部大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2010-11-06
• [Presentation] 「ミトコンドリア」を持たない寄生原虫・クリプトスポリジウムにおける呼吸鎖の生化学的解析 (2009)
  • Author(s): 原田倫世
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸ポートランド
  • Year and Date: 2009-10-22
• [Presentation] 「ミトコンドリア」を持たない寄生原虫・クリプトスポリジウムにおける呼吸鎖の生化学的解析 (2009)
  • Author(s): 原田倫世, 藤本陽子, 城戸康年, 坂元君年, 松崎素道, 籔義貞, 鈴木高史, 笹原武史, 中井裕, 北潔
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22