Malarial parasite molecules essential for development from sporoziote to merozoite in the liver stage.

• Project/Area Number: 21590472
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: CHINZEI Yasuo 鈴鹿医療科学大学, 医用工学部, 教授 (60024709)
• Co-Investigator(Kenkyū-buntansha): YUDA Masao 三重大学, 大学院・医学系研究科, 教授 (90293779) ; IWANAGA Shiro 三重大学, 大学院・医学系研究科, 准教授 (20314510) ; OOTSUKI Makoto 鈴鹿医療科学大学, 保健衛生学部, 助教 (60367878)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: マラリア / スポロゾイト / 肝内型原虫 / 遺伝子発現 / ワクチン

Research Abstract

We established the procedures for isolation of exoerithrocytic form parasites from the infected liver preparations by the cell sorter. We also established the expressed sequence tags database of the exoerythrocytic form parasites in the liver stage from sporozoites to merozotes. We analyzed a parasite molecule which functions to regulate the expression of the genes for development t omerozoites in the liver. Wa found this molecule is a transcription factor. We also showed the possibility of this molecule to be a candidate for development of EEF vaccine.

Research Products

• [Journal Article] Production of IFN-by CD4+T cells in response to malaria antigens is IL-2-dependent (2012)
  • Author(s): Kimura D., Miyakoda M., Honma K., Yuda M., Chinzei Y., and Yui K.
  • Journal Title: Int. Immunol Volume: 25 Pages: 2345-2356
  • NAID: 120006984811
• [Journal Article] Centromere Plasmid : A New Genetic Tool for the Study of Plasmodium falciparum (2012)
  • Author(s): Iwanaga S, Kato T, Kaneko I, Yuda M
  • Journal Title: PLoS One Volume: 7 Pages: 33326-33334
  • Peer Reviewed
• [Journal Article] Production of IFN-□□by CD4+ T cells in response to malaria antigens is IL-2-dependent (2012)
  • Author(s): Kimura D., Miyakoda M., Honma K, Yuda M., Chinzei Y., Yui K.
  • Journal Title: Int.Immunol Volume: 22 Pages: 941-952
  • NAID: 120006984811
  • Peer Reviewed
• [Journal Article] A high-coverage artificial chromosome library for the genome-wide screeing of drug-resistance genes in malaria parasites (2012)
  • Author(s): Iwanaga S, Kaneko I, Yuda M
  • Journal Title: Genome Res Volume: 22 Pages: 985-992
  • Peer Reviewed
• [Journal Article] Production of IFN-_Y by CD4_+ T cells in response to malaria antigens is IL-2-dependent (2011)
  • Author(s): Daisuke Kimura1, Mana Miyakoda1, Kiri Honma1, Masao Yuda2, Yasuo Chinzei2, Katsuyuki Yui1
  • Journal Title: International Imunology Volume: 7(In press)
  • Peer Reviewed
• [Journal Article] Waters AP Functional Identification of the Plasmodium Centromere and Generation of a Plasmodium Artificial Chromosome (2010)
  • Author(s): Iwanaga S, Khan SM., Kaneko I, Christodoulou Z, Newbold C, Yuda M, Janse CJ
  • Journal Title: Cell, Host & Microbe Volume: 7 Pages: 1215-1223
• [Journal Article] Functional Identification of the Plasmodium Centromere and Generation of a Plasmodium Artificial Chromosome. (2010)
  • Author(s): Iwanaga S, Khan SM, Kaneko I, Christodoulou Z, Newbold C, Yuda M, Janse CJ, Waters AP
  • Journal Title: Cell, Host & Microbe. 7(掲載確定)
  • Peer Reviewed
• [Journal Article] Transcription Factor AP2-Sp and its Target Genes in Malarial Sporozoites (2009)
  • Author(s): Yuda M, Iwanaga S, Shigenobu S, Kato T, Kaneko I.
  • Journal Title: Mol. Microbiol Volume: 75 Pages: 854-863
• [Journal Article] LISP1 is important for the egress of Plasmodium berghei parasites from liver cells (2009)
  • Author(s): Ishino T, Boisson B, Orito Y, Lacroix C, Bischoff E, Loussert C, Janse C, Menard R, Yuda M
  • Journal Title: Cell. Microbiol Volume: 11 Pages: 1329-1339
• [Journal Article] Transcription Factor AP2-Sp and its Target Genes in Malarial Sporozoites. (2009)
  • Author(s): Yuda M, Iwanaga S, Shigenobu S, Kato T, Kaneko I.
  • Journal Title: Mol.Microbiol. 75 Pages: 854-863
  • Peer Reviewed
• [Journal Article] LISP1 is important for the egress of Plasmodium berghei parasites from liver cells. (2009)
  • Author(s): Ishino T, Boisson B, Orito Y, Lacroix C, Bischoff E, Loussert C, Janse C, Menard R, Yuda M
  • Journal Title: Cell.Microbiol. 11 Pages: 1329-1339
  • Peer Reviewed
• [Patent(Industrial Property Rights)] マラリア原虫人工染色体を用いた薬剤耐性遺伝子の迅速同定法および組換えマラリア原虫作製法 (2011)
  • Inventor(s): 岩永史朗・油田正夫・金子伊澄
  • Industrial Property Rights Holder: 国立大学三重大学
  • Filing Date: 2011-03-26
  • Overseas
• [Patent(Industrial Property Rights)] マラリア原虫人工染色体を用いた薬剤耐性遺伝子の迅速同定法 (2010)
  • Inventor(s): 岩永史朗・油田正夫
  • Industrial Property Rights Holder: 国立大学三重大学
  • Industrial Property Number: 2010-071688
  • Filing Date: 2010-03-26
• [Patent(Industrial Property Rights)] マラリア原虫人工染色体 (2009)
  • Inventor(s): 岩永史朗・油田正夫
  • Industrial Property Rights Holder: 国立大学三重大学(90%)・国立大学鳥取大学(10%)
  • Industrial Property Number: 2009-051454
  • Filing Date: 2009-03-05