Simultaneous detection of plural cytokines production and gene expressions, in inflammatory reaction induced shock.

• Project/Area Number: 21590473
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Hyogo Medical University
• Principal Investigator: KASHIWAMURA Shinichiro 兵庫医科大学, 医学部, 准教授 (00185761)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ショック / 感染 / サイトカイン

Research Abstract

Inflammatory diseases, such as Rheumatoid Arthritis or infection induced septic shock decline patient's quality of life along with the progression of the diseases. To prevent the progression of diseases, rapid and precise diagnosis becomes an important matter. However, complex immune networks, mainly controlled with cytokines, obstruct proper understandings of those inflammatory diseases. In this study, I introduced newly developed flowcytometry-based suspension-array system which simultaneously measures 20-30 kind of target cytokines and proteins. Using this method, I clearly demonstrated that the abrogation of CD4+CD25+regulatory T cell function via glucocorticoid-induced TNF receptor(GITR) were major immune modulators those induced excess productions of various kinds of cytokine and deterioration of inflammatory diseases.

Research Products

• [Journal Article] Cisplatin-induced acute renal failure in mice is mediated by chymase-activated angiotensin-aldosterone system and interleukin-18 (2012)
  • Author(s): Okui S, Yamamoto H, Li W, Gamachi N, Fujita Y, Kashiwamura S, Miura D, Takai S, Miyazaki M, Urade M, Okamura H, Ueda H.
  • Journal Title: Eur J Pharmacol Volume: 685 Pages: 149-55
  • Peer Reviewed
• [Journal Article] Glucocorticoid-Induced TNF receptor-triggered T cells are key modulators for survival/death of neural stem/progenitor cells induced by ischemic stroke (2012)
  • Author(s): Takata M, Nakagomi T, Kashiwamura S, Nakano-Doi A, Saino O, Nakagomi N, Okamura H, Taguchi A, Matsuyama T.
  • Journal Title: Cell Death Differ Volume: 19 Pages: 756-67
  • Peer Reviewed
• [Journal Article] Abrogation of Treg function deteriorates rheumatoid arthritis (2012)
  • Author(s): Yamagiwa T, Fukunishi S, Tachibana T, Okamura H, Kashiwamura S.
  • Journal Title: Mod Rheumatol Volume: 22 Pages: 80-88
  • NAID: 10030206944
  • Peer Reviewed
• [Journal Article] Immunoregulatory effects of adsorptive granulocyte and monocyte apheresis in patients with drug refractory Crohns disease (2011)
  • Author(s): Nagase K, Fukunaga K, Kashiwamura S, Kono T, Kamikozuru K, Yokoyama Y, Hida N, Ohda Y, Takeda N, Yoshida K, Iimuri M, Kikuyama R, Kato K, Miwa H, Matsumoto T.
  • Journal Title: The Apher Dial Volume: 15 Pages: 367-73
  • Peer Reviewed
• [Journal Article] Infliximab therapy impacts the periphe ral immune-system of immunomodulator and corticosteroid naive patients with Crohns disease (2011)
  • Author(s): Kato K, Fukunaga K, Kamikozuru K, Kashiwa mura S, Hida N, Ohda Y, Takeda N, Yoshida K, Iimuro M, Kikuyama R, Miwa H, Mastumoto T
  • Journal Title: Gut and Liver Volume: 5 Pages: 37-45
  • Peer Reviewed
• [Journal Article] Infliximab therapy impacts the peripheral immune-system of immunomodulator and corticosteroid naive patients with Crohn's disease (2011)
  • Author(s): Kato K, et al
  • Journal Title: Gut and Liver Volume: VOL,5 Issue: 1 Pages: 37-45
  • DOI: 10.5009/gnl.2011.5.1.37
  • Peer Reviewed
• [Journal Article] Immunoregulatory effects of adsorptive granulocyte and monocyte apheresis inpatients with drug refractory Crohn's disease (2011)
  • Author(s): Nagase K, et al
  • Journal Title: Ther.Apher.Dial. Volume: VOL.15 Issue: 4 Pages: 367-73
  • DOI: 10.1111/j.1744-9987.2011.00970.x
  • Peer Reviewed
• [Journal Article] Imunodeficiency reduces neural stem/progenitor cell apoptosis in the cerebral cortex after stroke (2010)
  • Author(s): Saino O, Taguchi A, Nakagomi T, Nakano-Doi A, Kashiwamura S, Doe N, Nakagomi N, Soma T, Yoshikawa H, Stern DM, Okamura H, Matsuyama T.
  • Journal Title: J Neuroscience Res Volume: 88 Pages: 2385-97
  • Peer Reviewed
• [Journal Article] Immunodeficiency reduces neural stem/progenitor cell apoptosis and enhances neurogenesis in the cerebral cortex after stroke. (2010)
  • Author(s): Saino O, et al.
  • Journal Title: Journal of Neuroscience Research Volume: 88 Pages: 2385-2397
  • Peer Reviewed
• [Journal Article] 感染性ショックの誘導に関わるサイトカイン動態の多項目解析 (2009)
  • Author(s): 柏村信一郎, 平田淳一, 大家宗彦, 岡村春樹, 丸川征四郎
  • Journal Title: Cytometry Research Volume: 19 Pages: 61-66
  • NAID: 10024783104
  • Peer Reviewed
• [Journal Article] 感染性ショックの誘導に関わるサイトカイン動態の多項目解析 (2009)
  • Author(s): 柏村信一郎, 他
  • Journal Title: Cytometry Research 19 Pages: 61-66
  • NAID: 10024783104
  • Peer Reviewed
• [Presentation] 免疫制御性T細胞に着目した急性期・寛解期および外科手術後潰瘍性大腸炎の免疫的病態の検討 (2011)
  • Author(s): 横山陽子, 福永健, 柏村信一郎, 上小鶴孝二, 菊山梨紗, 吉田幸治, 武田直久, 飯室正樹, 應田義雄, 樋田信幸, 中村志郎, 三輪洋人, 松本譽之
  • Organizer: (シンポジウム)第48回日本消化器免疫学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2011-07-21
• [Presentation] 次世代白血球除去療法を目指した温熱刺激顆粒球・単球吸着療法の基礎検討:オンライン免疫調節治療の可能性 (2011)
  • Author(s): 福永健, 加藤恭一, 柏村信一郎, 松本譽之
  • Organizer: (シンポジウム)第48回日本消化器免疫学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2011-07-21
• [Presentation] 次世代白血球除去療法を目指した温熱刺激顆粒球・単球吸着療法の基礎検討:オンライン免疫調節治療の可能性 (2011)
  • Author(s): 福永健, 他
  • Organizer: (シンポジウム)第48回日本消化器免疫学会総会
  • Place of Presentation: 金沢市(石川県)
  • Year and Date: 2011-07-21
• [Presentation] 免疫制御性T細胞に着目した急性期・寛解期および外科手術後潰瘍性大腸炎の免疫学的病態の検討 (2011)
  • Author(s): 横山陽子, 他
  • Organizer: (シンポジウム)第48回日本消化器免疫学会総会
  • Place of Presentation: 金沢市(石川県)
  • Year and Date: 2011-07-21
• [Presentation] Transition of peripheral immune-characteristics pre, post to trans-operation in ulceractive colitis (2011)
  • Author(s): Yokoyama Y, Fukunaga K, Kashiwamura S, Kamikozuru K, Kikuyama R, Kato K, Takeda N, Mastumoto T.
  • Organizer: 15th International Congress of Mucosal Immunology(ICMI2011)
  • Place of Presentation: Paris
  • Year and Date: 2011-07-07
• [Presentation] Transition of peripheral immune-characteristics pre, post to trans-operation in ulceractive colitis (2011)
  • Author(s): Yokoyama Y, et al
  • Organizer: 15^<th> International Congress of Mucosal Immunology (ICMI2011)
  • Place of Presentation: Paris(フランス)
  • Year and Date: 2011-07-07
• [Presentation] Glucocorticoid-induced TNF receptor-stimulated T cells serve as a negative regulator for CNS repair after cerebral infraction (2011)
  • Author(s): Takata M, Nakagomi T, Kashiwamura S, Nakano-Doi A, Saino O, Okamura H, Mimura O, Soma T, Taguchi A, Matsuyama T.
  • Organizer: XXVth International Symposium on Cerebral Blood Flow, Metabolism and Function & Xth Inter ational Conference on Quantification of Brain Function and PET(Brain 2011)
  • Place of Presentation: Barcelona
  • Year and Date: 2011-05-27
• [Presentation] Glucocorticoid-induced TNF receptor-stimulated T cells serve as a negative regulator for CNS repair after cerebral infraction (2011)
  • Author(s): Takata M, et al
  • Organizer: XXVth International Symposium on Cerebral Blood Flow (Brain 2011)
  • Place of Presentation: Barcelona(スペイン)
  • Year and Date: 2011-05-27
• [Presentation] The transition of peripheral immune-characteristic in ulceractive colitis patients at active, quiescent and trans-colectomy (2011)
  • Author(s): Yokoyama Y, Fukunaga K, Kashiwamura S, Kamikozuru K, Miwa H, Mastumoto T.
  • Organizer: Digestive Disease Week 2011(DDW2011)
  • Place of Presentation: Chicago
  • Year and Date: 2011-05-10
• [Presentation] The transition of peripheral immune-characteristics in ulceractive colitis patients at active, quiescent and trans-colectomy (2011)
  • Author(s): Yokoyama Y, et al
  • Organizer: Digestive Disease Week 2011 (DDW2011)
  • Place of Presentation: Chicago(米国)
  • Year and Date: 2011-05-10
• [Presentation] GITR(glucocorticoid-induced TNF receptor) triggering reduces, and its inhibition enhances, endogenious neurogenesis in post-stroke brain (2010)
  • Author(s): Takata M, Kashiwamura S, Nakagomi T, Saino O, Nakano-Doi A, Doe N, Nakagomi N, Okamura H, Mimura O, Taguchi A, Mastuyama T.
  • Organizer: 7th World Stroke Congress
  • Place of Presentation: Souel
  • Year and Date: 2010-10-14
• [Presentation] CD25-postive T lymphocytes are key immunomodulator of neurogenesis after stroke (2010)
  • Author(s): Matsuyama T, Saino O, Nakagomi T, Nakano-Doi A, Takata M, Kashiwamura S, Doe N, Nakagomi N, Mimura O, Taguchi A.
  • Organizer: 7th World Stroke Congress
  • Place of Presentation: Souel
  • Year and Date: 2010-10-14
• [Presentation] CD25-positive T lymphocytes are key immunomodulators of neurogenesis after stroke. (2010)
  • Author(s): Matsuyama T, et al.
  • Organizer: 7th World Stroke Congress
  • Place of Presentation: Seoul
  • Year and Date: 2010-10-14
• [Presentation] GITR(glucocorticoid-induced TNF receptor) triggering reduces, and its inhibition enhances, endogenious neurogenesis in post-stroke brain. (2010)
  • Author(s): Takata M, et al.
  • Organizer: 7th World Stroke Congress
  • Place of Presentation: Seoul
  • Year and Date: 2010-10-14
• [Presentation] 脳梗塞後の内因性神経再生におけるGITR(Glucocorticoid-induced TNF receptor)陽性T細胞の関与の検討 (2010)
  • Author(s): 田片将士, 柏村信一郎, 中込隆之, 斎野織恵, 土居亜紀子, 土江伸誉, 中込奈美, 岡村春樹, 三村治, 田口明彦, 松山知之
  • Organizer: 第33回日本神経科学大会・第20回日本神経化学会大会、合同大会(Neuro2010)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] 脳梗塞後の内因性神経再生におけるGITR(Glucocorticoid-induced TNF receptor)陽性T細胞の関与の検討 (2010)
  • Author(s): 田片将士, 他
  • Organizer: 第33回日本神経科学大会・第53回日本神経化学会大会・第20回日本神経回路学会大会、合同大会(Neuro2010)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] CD4陽性T細胞が脳梗塞後の内因性神経幹細胞に及ぼす影響の検討 (2010)
  • Author(s): 斎野織恵, 田口明彦, 中込隆之, 土居亜紀子, 田片将士, 柏村信一郎, 土江伸誉, 松山知之
  • Organizer: 第33回日本神経科学大会・第20回日本神経化学会大会、合同大会(Neuro2010)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] CD4陽性T細胞が脳梗塞後の内因性神経幹細胞に及ぼす影響の検討 (2010)
  • Author(s): 斎野織恵, 他
  • Organizer: 第33回日本神経科学大会・第53回日本神経化学会大会・第20回日本神経回路学会大会、合同大会(Neuro2010)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] Novel-path ological roles of IL-18 in inflammatory diseases (2010)
  • Author(s): Kashiwamura S, Li W, Okamura H.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-24
• [Presentation] Novel-pathological roles of IL-18 in inflammatory diseases. (2010)
  • Author(s): Kashiwamura S, et al.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-24
• [Presentation] 心にもワクチンを (2009)
  • Author(s): 新家荘平, 柏村信一郎
  • Organizer: 日本予防医学会・日本補完代替医療学会合同シンポジウム弟5回日本予防医学会講演会・第12回日本補完代替医療学会学術集会
  • Place of Presentation: 和歌山
  • Year and Date: 2009-11-23
• [Presentation] 心にもワクチンを (2009)
  • Author(s): 新家荘平、柏村信一郎
  • Organizer: 第5回日本予防医学会・日本補完代替医療学会合同シポジウム(招待講演)
  • Place of Presentation: 和歌山
  • Year and Date: 2009-11-23
• [Presentation] 実験的脳梗塞モデルにおける免疫動態の検討 (2009)
  • Author(s): 田片将士, 柏村信一郎, 中込隆之, 斎野織恵, 中野亜紀子, 中込奈美, 田口明彦, 笠原由紀子, 三村治, 松山知之
  • Organizer: 第21回日本脳循環代謝学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-20
• [Presentation] 実験的脳梗塞モデルにおける免疫動態の検討 (2009)
  • Author(s): 田片将士、柏村信一郎, 他
  • Organizer: 第21回 日本脳循環代謝学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-20
• [Presentation] The roles of IL-18 expressed in normal human keratinocytes (2009)
  • Author(s): Kashiwamura S-I, Hosotani Y, Okamura H
  • Organizer: The 9^<th> World Congress on Inflammation
  • Place of Presentation: Tokyo
  • Year and Date: 2009-07-08
• [Presentation] The roles of IL-18 expressed in normal human keratinocytes (2009)
  • Author(s): Kashiwamura S-I, et al.
  • Organizer: The 9th. World Congress on Inflammation.
  • Place of Presentation: Tokyo
  • Year and Date: 2009-07-08