| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
Genetic analysis of Bac31 and Bac43, which are the Sec-dependent class IIa-type enterococcal bacteriocins, were performed. The chimera mutants composed of two bacteriocins were constructed and analyzed. Two amino acid residues positioned at 13th and 24th were significance for the activities. The factors concerning the expression and secretion of bacteriocin were as follows, gene copy number, promoter activity, signal peptide, and host bacteria. Of the factors, the signal peptide was the most crucial for the secretion level of bacteriocin. The immunity (resistance) genes for Bac31 and Bac43 were confirmed using a newly-constructed gene-expression controllable plasmid vector named pHT7343A.
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