A mechanism for regulation of clostripain activity and modification of inflammatory response by the enzyme.
| Project/Area Number |
21590483
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Chugoku Gakuen University (2011)
Kagawa University (2009-2010) |
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Principal Investigator |
OKABE Akinobu 中国学園大学, 現代生活学部, 教授 (20093677)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Shigeru 中部大学, 応用生物学部, 准教授 (90314913)
NARIYA Hirofumi 香川大学, 医学部, 助教 (30452668)
SUZUKI Motoo 香川大学, 医学部, 助教 (80457340)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | ウェルシュ菌 / clostripain / プロテアーゼ / 病原因子 / 凝集性 / ウエルシュ菌 / Clostripain / クロストリパン / リンカーペプチド / ガス壊疽 / eat-meシグナル / リンカー・ペプチド |
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| Research Abstract |
To examine a pathogenic role of clostripain(Clp) produced by Clostridium perfringens, Clp mutant and wild-type strains were injected into the mouse femur. Both strains showed gas gangrene and leukostasis to a similar extent, but no significant difference in histological characteristics. We developed an in-frame deletion method, by which we constructed various mutant strains. To obtain various toxins and mutant enzymes in large quantities, we constructed xylose-inducible expression system. Although we attempted to analyze regulation of Clp activity by a linker peptide and also an effect of Clp on eat-me signal of polymorphonuclear leukocytes, we failed to obtain conclusive evidence. We found the Clp mutant aggregates upon heating. Addition of EDTA, an inhibitor of Clp, to the culture of the wild type strain caused self-aggregation.
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Report
(4 results)
Research Products
(23 results)
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[Presentation] High-level production and purification of clostripain expressed in a virulence-attenuated strain of Clostridium perfringens2011
Author(s)
Hiroaki Tanaka, Hirofumi Nariya, Motoo Suzuki, Shigeru Miyata, Eiji Tamai, Shinji Kosaka, Noriyasu Fukuoka, Hitoshi Houchi, and Akinobu Okabe
Organizer
XIII International Congress of Bacteriology and Applied Microbiology, International Union of Microbiological Societies Congresses 2011
Place of Presentation
Sapporo, Japan
Related Report
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