Study of Clostridium perfringens beta-tox in : binding to lipid-rafts and effect on signal transduction.
| Project/Area Number |
21590500
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Keiko 徳島文理大学, 薬学部, 助教 (90170315)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ウエルシュ菌 / β毒素 / THP-1細胞 / マイクロドメイン / ネクローシス / ホスホリパーゼC |
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| Research Abstract |
Incubation of THP-l cells with C. perfringens beta-toxin showed the formation of oligomer on lipid rafts. Beta-toxin induced the activation of endogenous phospholipase C and production of IP_3.IP_3-induced Ca^<2+> uptake caused the activation of calmodulin-calcineurin signaling pathway. As beta-toxin caused PI entry and cell swelling, beta-toxin produced cytotoxic activity through necrosis.
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Report
(4 results)
Research Products
(50 results)
