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Trial of developing an endotoxin bio-sensor

Research Project

Project/Area Number 21590501
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionMusashino University

Principal Investigator

MUROI Masashi  武蔵野大学, 薬学研究所, 准教授 (70311389)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsエンドトキシン / Toll様受容体 / マクロファージ / 免疫 / 細菌 / Toll-like receptor / lipopolysaccharide
Research Abstract

Development of an endotoxin bio-sensor for detecting dimerization of Toll-like receptor(TLR)/MD-2 complex was attempted. N-terminal(Nluc) or C-terminal(Cluc) half of luciferase was fused to either N-terminal or C-terminal of TLR4.Restoration of luciferase activity, which occurs when Nluc and Cluc come in close proximity, was not observed in response to endotoxin in cells transiently expressing these fusion proteins. Further analysis in a stable expression system may be required.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (51 results)

All 2012 2011 2010 2009

All Journal Article (20 results) (of which Peer Reviewed: 12 results) Presentation (30 results) Book (1 results)

  • [Journal Article] IRAK-1-mediated negative regulation of Toll-like receptor signaling through proteasome-dependent downregulation of TRAF62012

    • Author(s)
      Muroi M, Tanamoto K.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1823 Pages: 255-263

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] IRAK-1-mediated negative regulation of Toll-like receptor signaling through proteasome-dependent downregulation of TRAF62012

    • Author(s)
      Muroi M.
    • Journal Title

      Biochim.Biophys.Acta

      Volume: 1823 Pages: 255-263

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of matrix-assisted laser desorption ionization-time of flight mass spectrometry for discrimination of Escherichia strains possessing highly conserved ribosomal RNA gene sequences2011

    • Author(s)
      Muroi M., Shima K., Nakagawa Y., and Tanamoto K.
    • Journal Title

      Biol. Pharm. Bull

      Volume: 34 Pages: 430-432

    • NAID

      130000657825

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] IRAK-1によるTRAF6の分解を介したTLRシグナリングの抑制的調節2011

    • Author(s)
      室井正志
    • Journal Title

      エンドトキシン・自然免疫研究

      Volume: 14 Pages: 7-10

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Application of matrix-assisted laser desorption ionization-time of flight mass spectrometry for discrimination of Escherichia strains possessing highly conserved ribosomal RNA gene sequences2011

    • Author(s)
      Muroi M.
    • Journal Title

      Biol.Pharm.Bull.

      Volume: 34 Pages: 430-432

    • NAID

      130000657825

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effects of soluble MD-2 and soluble CD14 on bacterial growth2010

    • Author(s)
      Ohnishi T., Muroi M., Tanamoto K.
    • Journal Title

      Microbiol. Immunol

      Volume: 54 Pages: 74-80

    • NAID

      10029543968

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deoxynivalenol and nivalenol inhibit lipopolysaccharide-induced nitric oxide production by mouse macrophage cells2010

    • Author(s)
      Sugiyama K., Muroi M., Tanamoto K., Nishijima M., Sugita-Konishi Y
    • Journal Title

      Toxicol Lett

      Volume: 192 Pages: 150-4

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Urban aerosols induce pro-inflammatory cytokine production in macrophages and cause airway inflammation in vivo2010

    • Author(s)
      Yoshida, T., Yoshioka, Y., Fujimura, M., Kayamuro, H., Yamashita, K., Higashisaka, K., Nakanishi, R., Morishita, Y., Nabeshi, H., Yamashita, T., Muroi, M., Tanamoto, K., Nagano, K., Abe, Y., Kamada, H., Kawai, Y., Mayumi, T., Itoh, N., Yoshikawa, T., Tsun
    • Journal Title

      Biol. Pharm. Bull

      Volume: 33 Pages: 780-783

    • NAID

      130000248036

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 日本薬局方指定菌株の特性と保存管理法に関する研究III2010

    • Author(s)
      Tanamoto K., Muroi M., Igarashi M., Shima K., Nakagawa Y., Fujiwake H.
    • Journal Title

      医薬品医療機器レギュラトリーサイエンス

      Volume: 41 Pages: 890-894

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Journal Article] Urban aerosols induce pro-inflammatory cytokine production in macrophages and cause airway inflammation in vivo2010

    • Author(s)
      Yoshida, T.
    • Journal Title

      Biol.Pharm.Bull.

      Volume: 33 Pages: 780-783

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deoxynivalenol and nivalenol inhibit lipopolysaccharide-induced nitric oxide production by mouse macrophage cells2010

    • Author(s)
      Sugiyama K.
    • Journal Title

      Toxicol Lett. 192

      Pages: 150-154

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effects of soluble MD-2 and soluble CD14 on bacterial growth2010

    • Author(s)
      Ohnishi T.
    • Journal Title

      Microbiol. Immunol. 54

      Pages: 74-80

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Caspase-3 is activated and rapidly released from human umbilical vein endothelial cells in response to lipopolysaccharide2009

    • Author(s)
      Shioiri T., Muroi M., Hatao F., Nishida M., Ogawa T., Mimura Y., Seto Y., Kaminishi M., Tanamoto K.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1792 Pages: 1011-1018

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 日本薬局方指定菌株の特性と保存管理法に関する研究2009

    • Author(s)
      Tanamoto K., Muroi M., Nakagawa Y., Shima K., Ichimura K.
    • Journal Title

      Pharm. Regul. Sci

      Volume: 40 Pages: 520-524

    • Related Report
      2011 Final Research Report
  • [Journal Article] Caspase-3 is activated and rapidly released from human umbilical vein endothelial cells in response to lipopolysaccharide2009

    • Author(s)
      Shioiri T.
    • Journal Title

      Biochim. Biophys. Acta 1792

      Pages: 1011-1018

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      室井正志
    • Journal Title

      エンドトキシン研究11(医学図書出版)

      Pages: 17-19

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      杉山圭一
    • Journal Title

      エンドトキシン研究11(医学図書出版)

      Pages: 20-22

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      室井正志
    • Journal Title

      エンドトキシン研究12(医学図書出版)

      Pages: 69-71

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      大西貴弘
    • Journal Title

      エンドトキシン研究12(医学図書出版)

      Pages: 72-74

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      杉山圭一
    • Journal Title

      エンドトキシン研究12(医学図書出版)

      Pages: 81-83

    • Related Report
      2009 Annual Research Report
  • [Presentation] ミクログリア細胞におけるToll-like receptorの発現及び炎症性応答2012

    • Author(s)
      林原紀明、田村直樹、渡辺恵、室井正志、畑尾史彦, 瀬戸泰之, 棚元憲一
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎、長崎ブリックホール
    • Year and Date
      2012-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] ミクログリア細胞におけるToll-like receptorの発現及び炎症性応答2012

    • Author(s)
      林原紀明
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] ダニアレルゲンの自然免疫応答に及ぼす影響2012

    • Author(s)
      北島孝明, 室井正志, 山下直美, 棚元憲一
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎、長崎ブリックホール
    • Year and Date
      2012-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] ダニアレルゲンの自然免疫応答に及ぼす影響2012

    • Author(s)
      北島孝明
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Trichothecene mycotoxins inhibit MyD88-independent pathways of Toll-like receptors2011

    • Author(s)
      Sugiyama, K., Kinoshita, M., Minai, Y., Muroi, M., Tanamoto, K. and Sugita-Konishi, Y
    • Organizer
      9th Joint Meeting of ICS-ISICR
    • Place of Presentation
      イタリア、Monash University Prato Centre
    • Year and Date
      2011-10-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Trichothecene mycotoxins inhibit MyD88-independent pathways of Toll-like receptors2011

    • Author(s)
      Sugiyama K.
    • Organizer
      9th Joint Meeting of ICS-ISICR
    • Place of Presentation
      Palazzo dei Congressi (Italy)
    • Year and Date
      2011-10-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] IRAK-1-mediated negative regulation of Toll-like receptor signaling2011

    • Author(s)
      Masashi Muroi and Ken-ichi Tanamoto
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌、札幌コンベンションセンター
    • Year and Date
      2011-09-08
    • Related Report
      2011 Final Research Report
  • [Presentation] IRAK-1-mediated negative regulation of Toll-like receptor signaling2011

    • Author(s)
      Muroi M.
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光散乱エンドトキシン測定法分析法バリデーション2011

    • Author(s)
      杉浦友香、廣野泰亮、室井正志、棚元憲一
    • Organizer
      日本防菌防黴学会第38回年次大会
    • Place of Presentation
      大阪、千里ライフサイエンスセンター
    • Year and Date
      2011-08-31
    • Related Report
      2011 Final Research Report
  • [Presentation] マトリックス支援レーザー脱離イオン化質量分析法による遺伝的近縁菌株の識別解析2011

    • Author(s)
      室井正志、島圭介、中川恭好、棚元憲一
    • Organizer
      日本防菌防黴学会第38回年次大会
    • Place of Presentation
      大阪、千里ライフサイエンスセンター
    • Year and Date
      2011-08-31
    • Related Report
      2011 Final Research Report
  • [Presentation] 光散乱エンドトキシン測定法分析法バリデーション2011

    • Author(s)
      杉浦友香
    • Organizer
      日本防菌防黴学会第38回年次大会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Year and Date
      2011-08-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] マトリックス支援レーザー脱離イオン化質量分析法による遺伝的近縁菌株の識別解析2011

    • Author(s)
      室井正志
    • Organizer
      日本防菌防黴学会第38回年次大会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Year and Date
      2011-08-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] マトリックス支援レーザー脱離イオン化質量分析法による遺伝的近縁菌株の識別解析2011

    • Author(s)
      室井正志、島圭介、中川恭好、棚元憲一
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡、ツインメッセ静岡
    • Year and Date
      2011-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] 光散乱エンドトキシン測定法における攪拌回転速度の検討2011

    • Author(s)
      杉浦友香、廣野泰亮、室井正志、棚元憲一
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡、グランシップ
    • Year and Date
      2011-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] マトリックス支援レーザー脱離イオン化質量分析法による遺伝的近縁菌株の識別解析2011

    • Author(s)
      室井正志
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      ツインメッセ静岡(静岡県)
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光散乱エンドトキシン測定法における攪拌回転速度の検討2011

    • Author(s)
      杉浦友香
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      ツインメッセ静岡(静岡県)
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] IRAK-1によるTRAF6の分解を介したTLRシグナリングの抑制的調節2010

    • Author(s)
      室井正志、棚元憲一
    • Organizer
      第16回日本エンドトキシン・自然免疫研究会
    • Place of Presentation
      奈良、かしはら万葉ホール
    • Year and Date
      2010-11-13
    • Related Report
      2011 Final Research Report
  • [Presentation] IRAK-1によるTRAF6の分解を介したTLRシグナリングの抑制的調節2010

    • Author(s)
      室井正志
    • Organizer
      第16回日本エンドトキシン・自然免疫研究会
    • Place of Presentation
      かしはら万葉ホール(奈良県)
    • Year and Date
      2010-11-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] Stimulation of Toll-like receptors leads to proteasome-dependent downregulation of TRAF6 through interaction with IRAK-12010

    • Author(s)
      Masashi Muroi and Ken-ichi Tanamoto
    • Organizer
      Annual Meeting of The Society for Leukocyte Biology & The International Endotoxin and Innate Immunity Society
    • Place of Presentation
      バンクーバー、Fairmont Hotel
    • Year and Date
      2010-10-09
    • Related Report
      2011 Final Research Report
  • [Presentation] Stimulation of Toll-like receptors leads to proteasome-dependent down regulation of TRAF6 through interaction with IRAK-12010

    • Author(s)
      Muroi M.
    • Organizer
      Annual Meeting of The Society for Leukocyte Biology & The International Endotoxin and Innate Immunity Society
    • Place of Presentation
      Vancouver(Canada)
    • Year and Date
      2010-10-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] IRAK-1により誘導されるTRAF6のproteasome依存性の分解2010

    • Author(s)
      室井正志、棚元憲一
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山、桃太郎アリーナ
    • Year and Date
      2010-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] TRAF6はIRAK-1と相互作用することによりproteasome依存性に分解される2010

    • Author(s)
      室井正志、棚元憲一
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Year and Date
      2010-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] TRAF6はIRAK-1と相互作用することによりproteasome依存性に分解される2010

    • Author(s)
      室井正志
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] IRAK-1により誘導されるTRAF6のproteasome依存性の分解2010

    • Author(s)
      室井正志
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山県桃太郎アリーナ(岡山県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] TLRシグナルに対するdeoxynivalenolの抑制機構2009

    • Author(s)
      杉山圭一、室井正志、棚元憲一、小西良子
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸、神戸国際展示場
    • Year and Date
      2009-10-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 非病原性細菌による感染症に対する内分泌かく乱候補物質の影響評価2009

    • Author(s)
      水谷紀子、室井正志、五十嵐ありさ、菅野慎二、鎌田洋一、小西良子、棚元憲一
    • Organizer
      第16回日本免疫毒性学会学術大会
    • Place of Presentation
      旭川、市民文化会館
    • Year and Date
      2009-08-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 非結晶性ナノシリカの自然免疫抑制作用に関する検討2009

    • Author(s)
      森重智弘、吉岡靖雄、田辺綾、堤康央、室井正志、棚元憲一、河合裕一、眞弓忠範、向洋平、岡田直貴、中川晋作
    • Organizer
      第36回日本トキシコロジー学会
    • Place of Presentation
      盛岡、アイーナ
    • Year and Date
      2009-07-17
    • Related Report
      2011 Final Research Report
  • [Presentation] 非結晶性ナノシリカの自然免疫抑制作用に関する検討2009

    • Author(s)
      森重智弘
    • Organizer
      第36回日本トキシコロジー学会
    • Place of Presentation
      岩手県民情報交流センター(岩手県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 非病原性細菌による感染症に対する内分泌かく乱候補物質の影響評価2009

    • Author(s)
      水谷紀子
    • Organizer
      第16回日本免疫毒性学会学術大会
    • Place of Presentation
      旭川市民文化会館(北海道)
    • Related Report
      2009 Annual Research Report
  • [Presentation] TLRシグナルに対するdeoxynivalenolの抑制機構2009

    • Author(s)
      杉山圭一
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2009 Annual Research Report
  • [Book] IRAK-1によるTRAF6の分解を介したTLRシグナリングの抑制的調節、エンドトキシン・自然免疫研究2011

    • Author(s)
      室井正志、棚元憲一
    • Publisher
      医学図書出版
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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