Evolutionary path of streptococcal and staphylococcal superantigens

• Project/Area Number: 21590503
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Research Institute, International Medical Center of Japan
• Principal Investigator: AKIYAMA Toru 独立行政法人国立国際医療研究センター, (研究所), 室長 (20246466)
• Co-Investigator(Kenkyū-buntansha): KIRIKAE Teruo 独立行政法人国立国際医療研究センター, (研究所), 部長 (50192563)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: レンサ球菌 / スーパー抗原 / ゲノム / 進化 / ゲノム解析

Research Abstract

Molecular evolution path of bacterial superantigen(SAG), which contribute the virulence of Streptococcus and Staphylococcus aureus(SA) remains to be elucidated. In the present study, whole genome sequence of group G streptococcus(GGS) was determined for the first time. It was found that GGS is closet relative of GAS in the bacteria ever sequenced. Based on synteny analysis of GGS and GAS, as well as SA, it is strongly suggested that SPEG and SMEZ are the direct descendant of common ancestor of streptococcal SAG while SELW and SSL are the direct descendant of common ancestor of staphylococcal SAG.

Research Products

• [Journal Article] Complete Genome Sequence of Melissococcus plutonius DAT561, a Strain That Shows an Unusual Growth Profile and Is Representative of an Endemic Cluster in Japan (2012)
  • Author(s): Okumura K, Arai R, Okura M, Kirikae T, Takamatsu D, Osaki M, Miyoshi-Akiyama T.
  • Journal Title: J Bacteriol Volume: 194(11) Issue: 11 Pages: 3014-3014
  • DOI: 10.1128/jb.00437-12
  • Peer Reviewed
• [Journal Article] Complete genome sequence of Melissococcus plutonius ATCC 35311 (2011)
  • Author(s): Okumura K, Arai R, Okura M, Kirikae T, Takamatsu D, Osaki M, Miyoshi-Akiyama T.
  • Journal Title: J Bacteriol Volume: 193(15) Issue: 15 Pages: 4029-30
  • DOI: 10.1128/jb.05151-11
  • Peer Reviewed
• [Journal Article] Complete genome sequencing and analysis of a Lancefield group G Streptococcus dysgalactiae subsp. equisimilis strain causing streptococcal toxic shock syndrome(STSS) (2011)
  • Author(s): Shimomura Y, Okumura K, Murayama SY, Yagi J, Ubukata K, Kirikae T, Miyoshi-Akiyama T.
  • Journal Title: BMC Genomics Volume: 12 Issue: 1 Pages: 17-17
  • DOI: 10.1186/1471-2164-12-17
• [Journal Article] Complete genome sequencing and analysis of a Lancefield group G Streptococcus dysgalactiae subsp. equisimilis strain causing streptococcal toxic shock syndrome (STSS). (2011)
  • Author(s): Shimomura Y, Okumura K, Murayama SY, Yagi J, Ubukata K, Kirikae T, Miyoshi-Akiyama T.
  • Journal Title: BMC Genomics Volume: 12 Pages: 1-17
  • Peer Reviewed
• [Journal Article] Positive correlationbetween low adhesion of group A Streptococcus to mammalian cells and virulence in a mouse model (2009)
  • Author(s): Miyoshi-Akiyama T, Zhao J, Uchiyama T, Yagi J, Kirikae T.
  • Journal Title: FEMS Microbiol Lett 293(1) Pages: 107-114
  • Peer Reviewed
• [Presentation] Complete Genome Sequence of Melissococcus plutonius ATCC 35311 (2011)
  • Author(s): K.Okumura, R.Arai, M.Okura, T.Kirikae, D.Takamatsu, M.Osaki, T.Miyoshi-Akiyama
  • Organizer: 111th General Meeting of American Society for Microbiology
  • Place of Presentation: New Orleans, Louisiana, USA
• [Presentation] Complete Genome Sequence of Streptococcus dysgalactiae subsp. equisimilis Strain RE378 (2009)
  • Author(s): 村山そう明,秋山徹,下村有美,切替照雄,岡田圭祐,生方公子
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
• [Presentation] Experimental model of SDSE infection in CD46 transgenic mice (2009)
  • Author(s): 岡田圭祐,松井英則,吉田春乃,村山そう明,高橋哲史,中村正彦,秋山徹,切替照雄,内山竹彦,生方公子
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
• [Presentation] ゲノム解析からみたS. dysgalactiae subsp. equisimilisの病原性 (2009)
  • Author(s): 秋山徹
  • Organizer: 第58回日本感染症学会東日本地方会学術集会/第56回日本化学療法学会東日本支部総会合同学会
• [Presentation] レンサ球菌に存在するスーパー抗原の起源に関する解析 (2009)
  • Author(s): 奥村香世、下村有美、村山そう明、八木淳二、生方公子、切替照雄、秋山徹
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
• [Presentation] 黄色ブドウ球菌・溶連菌Streptococcus dysgalactiae subsp. equisimilis GGS_124株の全ゲノム配列の決定 (2009)
  • Author(s): 下村有美、奥村香世、村山そう明、八木淳二、生方公子、切替照雄、秋山徹
  • Organizer: 第82回日本細菌学会
  • Place of Presentation: 横浜
• [Presentation] Origin of Streptococcal Superantigens Based on Mass Genome Comparison among Streptococcus pyogenes and S. dysgalactiae subsp equisimilis (2009)
  • Author(s): Miyoshi-Akiyama T, K. Okumura, Y. Shimomura, S. Murayama, J. Yagi, K. Ubukata, T. Kirikae
  • Organizer: American Society of Microbiology, 109th General Meeting
  • Place of Presentation: Phildaelphia, USA
• [Presentation] Origin of Streptococcal Superantigens Based on Mass Genome Comparison among Streptococcus pyogenes and S.dysgalactiae subsp.equisimilis (2009)
  • Author(s): Miyoshi-Akiyama T, K.Okumural, Y.Shimomural, S.Murayama, J.Yagi, K.Ubukata, T.Kirikae
  • Organizer: American Society of Microbiology, 109th General Meeting
  • Place of Presentation: 米国・フィラデルフィア
• [Presentation] ゲノム解析からみたS.dysgalactiae subsp.equisimilis の病原性 (2009)
  • Author(s): 秋山徹
  • Organizer: 第58回日本感染症学会東日本地方会学術集会/第56回日本化学療法学会東日本支部総会 合同学会
  • Place of Presentation: 東京