Development of immunotherapy and virotherapy against ATL

• Project/Area Number: 21590504
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Hokkaido University
• Principal Investigator: OHASHI Takashi 北海道大学, 遺伝子病制御研究所, 准教授 (10282774)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: HTLV-I / 成人T細胞白血病 / 動物モデル / 免疫治療 / ウイルス療法 / MHC-I / Single chain trimer / ATL / CTL / Tax / Epitope / Vaccinia

Research Abstract

The potential for novel therapies against Human T-cell leukemia virus type I (HTLV-I) induced-adult T-cell leukemia (ATL) has been assessed in a rat model of HTLV-I infection. The results indicate that the single chain trimer (SCT) of MHC-I linked to HTLV-I epitope peptide is able to induce HTLV-I-specific cytotoxic responses and that LC16m8Δ, a highly attenuated vaccinia strain, has an ability to lyse HTLV-I-infected T cells. These results should be useful for further verifying the strategies to fight against HTLV-I.

Research Products

• [Journal Article] Nuclear and cytoplasmic effects of human CRM1 on HIV-1 production in rat cells (2011)
  • Author(s): Nagai-Fukataki M, Ohashi T, Hashimoto I, Kimura T, Hakata Y, Shida H
  • Journal Title: Genes Cells Volume: 16 Pages: 203-216
• [Journal Article] Nuclear and cytoplasmic effects of human CRM1 on HIV-1 production in rat cells. (2011)
  • Author(s): Nagai-Fukataki M.
  • Journal Title: Genes Cells Volume: 16 Pages: 203-216
  • Peer Reviewed
• [Journal Article] Inhibitory effect of human TRIM5alpha on HIV-1 production (2010)
  • Author(s): Zhang X., Kondo M., Chen J., Miyoshi H., Suzuki H., Ohashi T., and Shida H
  • Journal Title: Microbes Infect Volume: 12 Pages: 768-777
• [Journal Article] Inhibitory effect of human TRIM5alpha on HIV-1 production. (2010)
  • Author(s): Zhang X.
  • Journal Title: Microbes Infect. Volume: 12 Pages: 768-777
  • Peer Reviewed
• [Journal Article] Induction of IL-10-and IFN-g-producing T-cell responses by autoreactive T-cells expressing human T-cell leukemia virus type I Tax (2009)
  • Author(s): Takatsuka N., Hasegawa A., Takamori A., Shimizu Y., Kato H., Ohashi T., Amagasa T., Masuda T., Kannagi M
  • Journal Title: Int. Immunol Volume: 21 Issue: 10 Pages: 1089-1100
  • DOI: 10.1016/j.micinf.2010.05.004
• [Journal Article] Functional analysis of Foxp3 and CTLA-4 expressing HTLV-1-infected cells in a rat model (2009)
  • Author(s): Takayanagi R., Ohashi T., and Shida H
  • Journal Title: Microbes Infect Volume: 11 Issue: 9 Pages: 964-972
  • DOI: 10.1093/intimm/dxp089
• [Journal Article] Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages (2009)
  • Author(s): Okada H., Zhang X., Fofana IB., Nagai M., Suzuki H., Ohashi T., and Shida H
  • Journal Title: Retrovirology Volume: 6 Issue: 12 Pages: 43-43
  • DOI: 10.1016/j.micinf.2009.06.007
• [Journal Article] Immunogenicity of newly constructed attenuated vaccinia strain LC16m8△ that expresses SIV Gag protein (2009)
  • Author(s): Suzuki H., Kidokoro M., Fofana IB., Ohashi T., Okamura T., Honda M., Yamamoto N., and Shida H
  • Journal Title: Vaccine Volume: 27 Issue: 1 Pages: 966-971
  • DOI: 10.1186/1742-4690-6-43
• [Journal Article] Induction of IL-10- and IFN-γ-producing T-cell responses by autoreactive T-cells expressing human T-cell leukemia virus type I Tax. (2009)
  • Author(s): Takatsuka N
  • Journal Title: International Immunology 21 Pages: 1089-1100
  • Peer Reviewed
• [Journal Article] Functional analysis of Foxp3 and CTLA-4 expressing HTLV-1-infected cells in a rat model. (2009)
  • Author(s): Takayanagi R.
  • Journal Title: Microbes and Infection 11 Pages: 964-972
  • Peer Reviewed
• [Journal Article] Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages. (2009)
  • Author(s): Okada H.
  • Journal Title: Retrovirology 6 Pages: 43-43
  • Peer Reviewed
• [Journal Article] Immunogenicity of newly constructed attenuated vaccinia strain LC16m8Δ that expresses SIV Gag protein. (2009)
  • Author(s): Suzuki H.
  • Journal Title: Vaccine 27 Pages: 966-971
  • Peer Reviewed
• [Presentation] ラットモデルを用いた低病原性ワクシニアウイルスの抗HTLV-I腫瘍効果の解析 (2011)
  • Author(s): 大橋貴、中村貴史、木所稔、志田壽利
  • Organizer: 第4回HTLV-I研究会
  • Place of Presentation: 東京大学弥生講堂
  • Year and Date: 2011-09-19
• [Presentation] ラットモデルを用いた低病原性ワクシニアウイルスの抗HTLV-I腫瘍効果の解析 (2011)
  • Author(s): 大橋貴
  • Organizer: 第4回HTLV-I研究会
  • Place of Presentation: 東京大学弥生講堂(東京)
  • Year and Date: 2011-09-19
• [Remarks]
  • URL: http://www.igm.hokudai.ac.jp/molvir/index.html
• [Remarks]
  • URL: http://www.igm.hokudai.ac.jp/molvir/index.html
• [Remarks]
  • URL: http://www.igm.hokudai.ac.jp/molvir/index.html
• [Remarks]
  • URL: http://www.igm.hokudai.ac.jp/molvir/index.html