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Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway.

Research Project

Project/Area Number 21590507
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionThe University of Tokyo

Principal Investigator

MIZUTANI Taketoshi  東京大学, 医科学研究所, 助教 (00376617)

Co-Investigator(Kenkyū-buntansha) IBA Hideo  東京大学, 医科学研究所, 教授 (60111449)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsSWI/SNF複合体 / HIV-1 / NF-κB / レトロウイルス / SWI/SNF / NF-kappaB / SWI / SNF
Research Abstract

In this study, we identify the human requiem protein(REQ/DPF2) as an adaptor molecule that links the NF-kappaB and SWI/SNF chromatin remodeling factor.
Using sensitive 293FT reporter cell clones that had integrated a SWI/SNF-dependent NF-κB reporter gene, we find in this study that the overexpression of DPF1, DPF2, DPF3a, DPF3b, and PHF10 significantly potentiates the transactivating activity of typical NF-κB dimers. Knockdown analysis using 293FT reporter cells that endogenously express these five proteins at low levels clearly showed that DPF3a and DPF3b, which are produced from the DPF3 gene by alternative splicing, are the most critical for the RelA/p50 NF-κB heterodimer transactivation induced by TNF-αstimulation. Our data further show that this transactivation requires the SWI/SNF complex. DPF3a and DPF3b are additionally shown to interact directly with RelA, p50, and several subunits of the SWI/SNF complex in vitro and to be co-immunoprecipitated with RelA/p50 and the SWI/SNF complex from the nuclear fractions of cells treated with TNF-α. In ChIP experiments, we further found that endogenous DPF3a/b and the SWI/SNF complex are continuously present on HIV-1 LTR, whereas the kinetics of RelA/p50 recruitment after TNF-αtreatment correlate well with the viral transcriptional activation levels.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (11 results)

All 2012 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] Double PHD finger proteins DPF3a and 3b are reouired as transcriptional coactivators in the SWI/SNF complex-dependent activation of the NF-κB Re1A/p50 heterodimer2012

    • Author(s)
      Ishizaka A, Mizutani T, Kobayashi K, Tando T, Sakurai K, Fujiwara T, Iba H
    • Journal Title

      The Journal of Biological Chemistry

      Volume: (印刷中) Issue: 15 Pages: 11924-11933

    • DOI

      10.1074/jbc.m111.322792

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Double Plant Homeodomain (PHD) Finger Proteins DPF3a and -3b Are Required as Transcriptional Co-activators in SWI/SNF Complex-dependent Activation of NF-κB RelA/p50 Heterodimer2012

    • Author(s)
      Ishizaka A, Mizutani T, Kobayashi K, Tando T, Sakurai K, Fujiwara T, Iba H
    • Journal Title

      J.Biol.Chem.

      Volume: 287(15) Pages: 11924-11933

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a non-canonical NF-〓B pathway.2010

    • Author(s)
      ando T, Ishizaka A, Watanabe H, Ito T, Iida S, Haraguchi T, Mizutani T, Izumi T, Isobe T, Akiyama T, Inoue J and Iba H.
    • Journal Title

      J. Biol. Chem

      Volume: 285 Issue: 29 Pages: 21951-60

    • DOI

      10.1074/jbc.m109.087783

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Requiem protein links ReIB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway.2010

    • Author(s)
      Tando T, Ishizaka A, Watanabe H, Ito T, Iida S, Haraguchi T, Mizutani T, Izumi T, Isobe T, Akiyama T, Inoue J, Iba H.
    • Journal Title

      J. Biol. Chem.

      Volume: 285(29) Pages: 21951-21960

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Loss of the Brm-Type SWI/SNF Chromatin Remodeling Complex Is a Strong Barrier to the Tat-Independent Transcriptional Elongation of Human Immunodeficiency Virus Type 1 Transcripts2009

    • Author(s)
      Mizutani T, Ishizaka A, Tomizawa M, Okazaki T, Yamamichi N, Kawana-Tachikawa A, Iwamoto A, Iba H
    • Journal Title

      JOURNAL OF VIROLOGY 83

      Pages: 11569-11580

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] HIV-のTat非依存的な発現におけるSWI/SNF複合体の作用点の解析2010

    • Author(s)
      水谷壮利、石坂彩、伊庭英夫
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      徳島県あわぎんホール
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Analysis for the regulation of transcriptional elongation of HIV-1 transcript by proviral non-coding RNA2010

    • Author(s)
      Mizutani T, Ishizaka A, and Iba H
    • Organizer
      The 10th Awajishima International Forum on Infection and Immunity
    • Place of Presentation
      兵庫県淡路島夢舞台国際会議場
    • Related Report
      2011 Final Research Report
  • [Presentation] Analysis for the regulation of transcriptional elongation of HIV-1 transcript by proyiral non-coding RNA2010

    • Author(s)
      Mizutani T, Ishizaka A, Iba H
    • Organizer
      The 10^<th> Awajishima International Forum on Infection and Immunity
    • Place of Presentation
      兵庫県淡路島夢舞台国際会議場
    • Related Report
      2010 Annual Research Report
  • [Presentation] The Brm-type SWI/SNF chromatin remodeling complex plays an important role in transcriptional elongation of HIV-1 in the absence of Tat.2009

    • Author(s)
      Ishizaka A, Mizutani T, Tomizawa M, Kawana-Tachikawa A, Iwamoto A, Iba H
    • Organizer
      The 9^<th> Awajishima International Forum on Infection and Immunity
    • Place of Presentation
      兵庫県 淡路島夢舞台国際会議場
    • Related Report
      2009 Annual Research Report
  • [Presentation] HIVのTat非依存的な発現におけるSWI/SNF複合体の作用点の解析2009

    • Author(s)
      水谷壮利、石坂彩、富沢麻利子、立川(川名)愛、岩本愛吉、伊庭英夫
    • Organizer
      第57回 日本ウイルス学会学術集会
    • Place of Presentation
      東京都 都市センターホテル
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ims.u-tokyo.ac.jp/div-host-parasite/Version1.html

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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