Study on proteasome-dependent regulation of viral infection

• Project/Area Number: 21590508
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: University of Yamanashi (2010-2011); Osaka University (2009)
• Principal Investigator: MORIISHI Kohji 山梨大学, 大学院・医学工学総合研究部, 教授 (90260273)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: プロテアソーム / フラビウイルス / ウイルス複製 / ウイルス感染 / C型肝炎ウイルス / フラビウイル / フラピウイルス / ブロテアソーム

Research Abstract

It has been unknown the mechanism by which ubiquitin-independent proteasome regulates hepatitis C virus(HCV) infection. HCV propagation was suppressed by inhibition of ubiquitin-independent proteasome in cell culture system. Furthermore, ubiquitin-independent proteasome associated with cell proliferation and DNA repair in the liver of mouse model. These data suggest that ubiquitin-independent proteasome plays an important role in HCV infection and pathogenicity.

Research Products

• [Journal Article] Exploitation of lipid components by viral and host proteins for hepatitis C virus infection (2012)
  • Author(s): Moriishi K, Matsuura Y
  • Journal Title: Front. Microbiol. Volume: 3 Pages: 54-54
  • Peer Reviewed
• [Journal Article] Upregulation of nuclear PA28gamma expression in cirrhosis and hepatocelluar carcinoma (2012)
  • Author(s): Kondo M, Moriishi K, Wada H, Murabashi S, Wakasa K, Matsuura Y, Doki Y, Mori M, Nagano H
  • Journal Title: Exp. Ther. Med. Volume: 3 Pages: 379-385
  • Peer Reviewed
• [Journal Article] CD44 participates in the IP-10 induction in cells replicating HCV RNA through an interaction with TLR2 and hyaluronan (2012)
  • Author(s): Abe T, Fukuhara T, Wen X, Ninomiya A, Moriishi K, Maehara Y, Takeuchi O, Kawai T, Akira S, Matsuura Y
  • Journal Title: J. Virol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Exploitation of lipid components by viral and host proteins for hepatitis C virus infection (2012)
  • Author(s): Moriishi, K.
  • Journal Title: Frontiers in Virology Volume: 3 Pages: 54-54
  • DOI: 10.3389/fmicb.2012.00054
  • Peer Reviewed
• [Journal Article] Upregulation of nuclear PA28ganmia expression in cirrhosis and hepatocelluar carcinoma (2012)
  • Author(s): Kondo, M
  • Journal Title: Exp.Ther.Med. Volume: 3 Issue: 3 Pages: 379-385
  • DOI: 10.3892/etm.2011.415
  • Peer Reviewed
• [Journal Article] Elimination of hepatitis C virus from hepatocytes by a selective activation of therapeutic molecules (2011)
  • Author(s): Wen X, Abe T, Kukihara H, Taguwa S, Mori Y, Tani H, Kato N, Suzuki T Tatsumi M, Moriishi K, Matsuura Y
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Will there be an HCV meeting in 2020? Summary of the 17th international meeting on hepatitis C virus and related viruses (2011)
  • Author(s): Wakita T, Suzuki T, Evans MJ, Shimotohno K, Chayama K, Matsuura Y, Hijikata M, Moriishi K, Seya T, Enomoto N, Koike K, Kato N, Kanto T, Hotta H
  • Journal Title: Gastroenterology Volume: 141
  • Peer Reviewed
• [Journal Article] Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus (2011)
  • Author(s): Taguwa S, Kambara H, Fujita N, Noda T, Yoshimori T, Koike K, Moriishi K, Matsuura Y
  • Journal Title: J. Virol. Volume: 85 Pages: 13185-13194
  • Peer Reviewed
• [Journal Article] Involvement of cyclophilin B in the replication of Japanese encephalitis virus (2011)
  • Author(s): Kambara H, Tani H, Mori Y, Abe T, Katoh H, Fukuhara T, Taguwa S, Moriishi K, Matsuura Y
  • Journal Title: Virology Volume: 412 Pages: 211-219
  • Peer Reviewed
• [Journal Article] Network based analysis of hepatitis C virus core and NS4B protein interactions (2010)
  • Author(s): Tripathi LP, Kataoka C, Taguwa S, Moriishi K, Mori Y, Matsuura Y, Mizuguchi K
  • Journal Title: Mol. Biosyst. Volume: 6 Pages: 2539-2533
  • Peer Reviewed
• [Journal Article] Establishment of an indicator cell system for hepatitis C virus (2010)
  • Author(s): Tanaka Y, Mori Y, Tani H, Abe T, Moriishi K, Kojima H, Nagano T, Okabe T, Suzuki T, Tatsumi M, Matsuura Y
  • Journal Title: Microbiol. Immunol. Volume: 54 Pages: 206-220
  • Peer Reviewed
• [Journal Article] Involvement of PA28gamma in the propagation of hepatitis C virus (2010)
  • Author(s): Moriishi K, Shoji I, Mori Y, Suzuki R, Suzuki T, Kataoka C, Matsuura Y
  • Journal Title: Hepatology Volume: 52 Pages: 411-420
  • Peer Reviewed
• [Journal Article] Peripheral B cells may serve as a reservoir for persistent hepatitis C virus infection (2010)
  • Author(s): Ito M, Masumi A, Mochida K, Kukihara H, Moriishi K, Matsuura Y, Yamaguchi K, Mizuochi T
  • Journal Title: J. Innate Immun. Volume: 2 Pages: 607-617
  • Peer Reviewed
• [Journal Article] Network based analysis of hepatitis C virus core and NS4B protern interactlons. (2010)
  • Author(s): Tripathi LP
  • Journal Title: Mol Biosyst. Volume: 6 Pages: 2539-2553
  • Peer Reviewed
• [Journal Article] Peripheral B cells may serve as a reservoir for persistent hepatitis C virus infection. (2010)
  • Author(s): Ito M
  • Journal Title: J Innate Immun Volume: 2 Pages: 607-617
  • Peer Reviewed
• [Journal Article] Cochaperone activity of human butyrate-induced transcript 1 facilitates hepatitis C virus replication through an Hsp90-dependent pathway (2009)
  • Author(s): Taguwa S, Kambara H, Omori H, Tani H, Abe T, Mori Y, Suzuki T, Yoshimori T, Moriishi K, Matsuura Y
  • Journal Title: J. Virol. Volume: 83 Pages: 10427-10436
  • Peer Reviewed
• [Journal Article] Proteasomal turnover of hepatitis C virus core protein is regulated by two distinct mechanisms : a ubiquitin-dependent mechanism and a ubiquitin-independent but PA28gamma-dependent mechanism (2009)
  • Author(s): Suzuki R, Moriishi K, Fukuda K, Shirakura M, Ishii K, Shoji I, Wakita T, Miyamura T, Matsuura Y, Suzuki T
  • Journal Title: J. Virol. Volume: 83 Pages: 2389-2392
  • Peer Reviewed
• [Journal Article] Human VAP-C negatively regulates hepatitis C virus propagation (2009)
  • Author(s): Kukihara H, Moriishi K, Taguwa S, Tani H, Abe T, Mori Y, Suzuki T, Fukuhara T, Taketomi A, Maehara Y, Matsuura Y
  • Journal Title: J. Virol. Volume: 83 Pages: 7959-7969
  • Peer Reviewed
• [Journal Article] Baculovirus induces type I interferon production through toll-like receptor-dependent and-independent pathways in a cell-type-specific manner (2009)
  • Author(s): Abe T, Kaname Y, Wen X, Tani H, Moriishi K, Uematsu S, Takeuchi O, Ishii KJ, Kawai T, Akira S, Matsuura Y
  • Journal Title: J. Virol. Volume: 83 Pages: 7629-7640
  • Peer Reviewed
• [Journal Article] Tacrolimus ameliorates metabolic disturbance and oxidative stress caused by hepatitis C virus core protein : analysis using mouse model and cultured cells (2009)
  • Author(s): Moriya K
  • Journal Title: Am J Pathol. 175 Pages: 1515-15124
  • Peer Reviewed
• [Journal Article] Cochaperone activity of human butyrate-induced transcript 1 facilitates hepatitis C virus replication through an Hsp90-dependent pathway (2009)
  • Author(s): Taguwa S
  • Journal Title: J.Virol. 83 Pages: 10427-10436
  • Peer Reviewed
• [Journal Article] Human VAP-C negatively regulates hepatitis C virus propagation (2009)
  • Author(s): Kukihara H
  • Journal Title: J.Virol. 83 Pages: 7959-7969
  • Peer Reviewed
• [Presentation] Regulation of HCV replication by FKBP8-dependent or-independent Hsp90 activity (2011)
  • Author(s): Kawakami, K., Kasai, H., Yamashita, A., Enomoto, N., Matsuura, Y., Kusunoki, M., and Moriishi K.
  • Organizer: 第34回日本分子生物学会総会
  • Place of Presentation: 横浜
• [Presentation] Regulation of HCV replication by FKBP8-dependent or-independent Hsp90 activity (2011)
  • Author(s): Kawakami, K., Kasai, H., Yamashita, A., Enomoto, N., Matsuura, Y., Kusunoki, M., and Moriishi K.
  • Organizer: 18th International Sympodium on Hepatitis C virus and Related Viruses
  • Place of Presentation: Seattle. USA.
• [Presentation] Involvement of cyclophilin B in the replication of Japanese encephalitis virus (2011)
  • Author(s): Kambara H., Tani H., Mori Y., Abe T., Katoh H., Fukuhara T., Taguwa S., Moriishi K., and Matsuura Y.
  • Organizer: 2011 Sapporo
  • Place of Presentation: Japan
• [Presentation] Inhibitory effect of marine natural products on the replication of hepatitis C virus (2011)
  • Author(s): Fujimoto Y., Yamashita A., and Moriishi K.
  • Organizer: 2011 Sapporo
  • Place of Presentation: Japan
• [Presentation] Marine natural products as a source of the novel antiviral agent targeting to HCV NS3 helicase XV International Congress of Virology (2011)
  • Author(s): Yamashita A., Fujimoto Y., and Moriishi K.
  • Organizer: 2011 Sapporo
  • Place of Presentation: Japan
• [Presentation] HCVによる脂質代謝障害の分子機序 (2010)
  • Author(s): 森石恆司
  • Organizer: 第58回日本ウイルス学会
  • Place of Presentation: 徳島市
  • Year and Date: 2010-11-09
• [Presentation] Involvement of PA28gamma in the propagation of PA28gamma (2010)
  • Author(s): Moriishi K
  • Organizer: The 17th international symposium on hepatitis C virus and related viruses
  • Place of Presentation: 横浜市
  • Year and Date: 2010-09-14
• [Presentation] Mechanism of hepatitis C virus infection : interaction between viral and host proteins (2010)
  • Author(s): Moriishi K
  • Organizer: International symposium on organelle network : microbiology, immunology, and cell biology
  • Place of Presentation: 大阪市
  • Year and Date: 2010-07-13
• [Presentation] Involvement of PA28gamma in the propagation of HCV. (2010)
  • Author(s): Moriishi K., Shoji I., Mori Y., Suzuki R., Suzuki T., Kataoka C., Matsuura Y.
  • Organizer: 17th International Sympodium on Hepatitis C virus and Related Viruses
  • Place of Presentation: Yokohama. Japan
• [Presentation] HCV replication enhances secretion of an immature cathepsin B through induction of an incomplete autophagy (2010)
  • Author(s): Taguwa S., Kambara H., Fujita N., Noda T., Yoshimori T., Moriishi K., Matsuura Y.
  • Organizer: 17th International Sympodium on Hepatitis C virus and Related Viruses
  • Place of Presentation: Yokohama. Japan
• [Presentation] Inhibition of autophagy induces lysosomal vacuolation in cells replicating HCV (2010)
  • Author(s): Kambara H., Taguwa S., Fujia N., Noda T., Yoshimori T., Moriishi K., Matsuura Y.
  • Organizer: 17th International Sympodium on Hepatitis C virus and Related Viruses
  • Place of Presentation: Yokohama. Japan
• [Presentation] 核小体蛋白質B23はC型肝炎ウイルス複製を抑制する (2010)
  • Author(s): 加藤大志、森嘉生、寒原裕登、要祐喜、谷英樹、阿部隆之、神谷亘、森石恆司、松浦善治
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] C型肝炎ウイルスはオートファジーを誘導して持続感染を成立させる (2010)
  • Author(s): 寒原裕登、田鍬修平、藤田尚信、野田健司、森石恆司、吉森保、松浦善治
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] HCVによる脂質代謝障害の分子機序 (2010)
  • Author(s): 森石恆司、松浦善治
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] 宿主タンパクのHCV産生制御 (2010)
  • Author(s): 森石恆司
  • Organizer: 北大遺伝子病制御研究所、研究集会「感染と癌」
  • Place of Presentation: 札幌
• [Presentation] Suppression of HCV Replication in Hepatocytes through a Selective Induction of IRF7 (2009)
  • Author(s): Wen X, Abe T, Taguwa S, Mori Y, Moriishi K, Matsuura Y.
  • Organizer: 16th International Sympodium on Hepatitis C virus and Related Viruses
  • Place of Presentation: Nice, France
• [Presentation] Hyaluronan Participates in the IP-10 Induction in Cells Infected with HCV through an Engagement of TLR2 and CD44 (2009)
  • Author(s): Abe T, Kaname Y, Moriishi K, Kanto T, Hayashi N, Matsuura Y.
  • Organizer: 16th International Sympodium on Hepatitis C virus and Related Viruses
  • Place of Presentation: Nice, France
• [Presentation] Involvement of PA28gamma and E6AP in the Degradation of HCV Core Protein and the Viral Production (2009)
  • Author(s): Moriishi K, Shoji I, Suzuki R, Suzuki T, Matsuura Y
  • Organizer: 16th International Sympodium on Hepatitis C virus and Related Viruses
  • Place of Presentation: Nice, France
• [Presentation] HCVコア蛋白質のプロテアソームによる分解とウイルス産生制御 (2009)
  • Author(s): 森石恆司、勝二郁夫、鈴木亮介、鈴木哲朗、松浦善治
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
• [Presentation] ヒラルロン酸による炎症性ケモカインIP-10の過剰産生とC型肝炎の慢性化 (2009)
  • Author(s): 阿部隆之、要祐喜、森石恆司、考藤達哉、林紀夫、松浦善治
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
• [Presentation] 宿主蛋白質を利用したC型肝炎ウイルスの感染戦略 (2009)
  • Author(s): 森石恆司
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Involvement of PA28g and E6AP in the degradation of HCV core protein and the viral production (2009)
  • Author(s): 森石恆司
  • Organizer: The 16th international symposium on hepatitis C virus and related viruses
  • Place of Presentation: フランス、ニース
• [Presentation] HCVコア蛋白質のプロテアソームによる分解とウイルス産生制御 (2009)
  • Author(s): 森石恆司
  • Organizer: 第57回日本ウイルス学会
  • Place of Presentation: 東京