Regulation of genome replication of negative-strand RNA virus by an accessory protein

• Project/Area Number: 21590510
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Hiroshima University
• Principal Investigator: SAKAGUCHI Takemasa 広島大学, 大学院・医歯薬学総合研究科, 教授 (70196070)
• Co-Investigator(Kenkyū-buntansha): IRIE Takashi 広島大学, 大学院・医歯薬学総合研究科, 准教授 (70419498) ; KIYOTANI Katsuhiro 広島大学, 大学院・医歯薬学総合研究科, 講師 (00106824)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ウイルス / パラミクソウイルス / センダイウイルス / ゲノム極性 / C蛋白質 / プロモーター / cDNAからのウイルス産生系 / cDNAからのウイルス作成系

Research Abstract

Infection experiments with the C protein-deficient Sendai virus, which has a single-stranded negative-sense RNA as a genome, suggested that the C protein was involved in regulation of RNA genome polarity during genome replication. The C protein appeared to suppress genomic RNA synthesis in a promoter-dependent fashion, resulting that progeny viruses dominantly had a negative-sense RNA genome. This regulation seems to be related with interferon-βinduction. We also tested other closely related viruses for the regulation.

Research Products

• [Journal Article] Inhibition of interferon regulatory factor-3 activation by paramyxovirus V protein (2012)
  • Author(s): Irie, T., Kiyotani, K., Igarashi, T., Yoshida, A. Sakaguchi, T.
  • Journal Title: Journal of Virology Volume: vol.86 Pages: 7136-7145
  • Peer Reviewed
• [Journal Article] Optineurin with amyotrophic lateral sclerosis-related mutations abrogates inhibition of interferon regulatory factor-3 activation (2011)
  • Author(s): Sakaguchi, T., Irie, T., Kawabata, R., Yoshida, A., Maruyama, H., Kawakami, H.
  • Journal Title: Neuroscience letters Volume: vol.505 Pages: 279-281
  • NAID: 120005308229
  • Peer Reviewed
• [Journal Article] Analysis of interaction of Sendai virus V protein and melanoma differentiation-associated gene 5 (2011)
  • Author(s): Sakaguchi, T., Irie, T., Kuwayama, M., Ueno, T., Yoshida, A., Kawabata, R.
  • Journal Title: Microbiology and Immunology Volume: vol.55 Pages: 760-767
  • NAID: 10031121530
  • Peer Reviewed
• [Journal Article] Decreased expression in nuclear factor-κB essential modulator due to a novel splice-site mutation causes X-linked ectodermal dysplasia with immunodeficiency (2011)
  • Author(s): Karakawa, S., Okada, S., Tsumura, M., Mizoguchi, Y., Ohno, N., Yasunaga, S., Otsubo, M., Kawai, T., Nishikomori, R., Sakaguchi, T., Takihara, T., Kobayashi, M.
  • Journal Title: Journal of Clinical Immunology Volume: vol.31 Pages: 762-772
  • Peer Reviewed
• [Journal Article] Oncolytic vesicular stomatitis virus administered by isolated limb perfusion suppresses osteosarcoma growth. (2011)
  • Author(s): Kubo, T., Shimose, S., Matsuo, T., Fujimori, J., Sakaguchi, T., Yamaki, M., Shinozaki, K., Woo, S. L., Ochi M.
  • Journal Title: Journal of Orthopaedic Research Volume: vol.29 Pages: 795-800
  • Peer Reviewed
• [Journal Article] Oncolytic vesicular stomatitis virus administered by isolated limb perfusion suppresses osteosarcoma growth (2011)
  • Author(s): Kubo, T., et al
  • Journal Title: Journal of Orthopaedic Research Volume: 29 Pages: 795-800
  • Peer Reviewed
• [Journal Article] Decreased expression in nuclear factor-κB essential modulator due to a novel splice-site mutation causes X-linked ectodermal dysplasia with immunodeficiency (2011)
  • Author(s): Karakawa, S., et al
  • Journal Title: Journal of Clinical Immunology Volume: 31 Pages: 762-772
  • Peer Reviewed
• [Journal Article] Analysis of interaction of Sendai virus V protein and melanoma differentiation-associated gene 5 (2011)
  • Author(s): Sakaguchi, T., et al
  • Journal Title: Microbiology and Immunology Volume: 55 Pages: 760-767
  • NAID: 10031121530
  • Peer Reviewed
• [Journal Article] Optineurin with amyotrophic lateral sclerosis-related mutations abrogates inhibition of interferon regulatory factor-3 activation (2011)
  • Author(s): Sakaguchi, T., et al
  • Journal Title: Neuroscience letters Volume: 505 Pages: 279-281
  • NAID: 120005308229
  • Peer Reviewed
• [Journal Article] Significance of the YLDL motif in the M protein and Alix/ AIP1 for Sendai virus budding in the context of virus infection. (2010)
  • Author(s): Irie, T., Inoue, M., Sakaguchi, T.
  • Journal Title: Virology Volume: vol.405 Pages: 334-341
  • Peer Reviewed
• [Journal Article] Paramyxovirus assembly and budding : building particles that transmit infections. (2010)
  • Author(s): Harrison, M. S., Sakaguchi, T., Schmitt, A. P.
  • Journal Title: The International Journal of Biochemistry & Cell Biology Volume: vol.42 Pages: 1416-1429
  • Peer Reviewed
• [Journal Article] Conserved charged amino acids within Sendai virus C protein play multiple roles in the evasion of innate immune responses (2010)
  • Author(s): Irie, T., Nagata, N., Igarashi, T., Okamoto, I., Sakaguchi, T
  • Journal Title: PLoS One Volume: vol.5 Pages: 10719-10719
  • NAID: 120002598781
  • Peer Reviewed
• [Journal Article] Conserved charged amino acids within Sendai virus C protein play multiple roles in the evasion of innate immune responses. (2010)
  • Author(s): Irie, T., et al.
  • Journal Title: PLoS One Volume: 5
  • NAID: 120002598781
  • Peer Reviewed
• [Journal Article] Paramyxovirus assembly and budding : building particles that transmit infections. (2010)
  • Author(s): Harrison, M.S., et al.
  • Journal Title: The International Journal of Biochemistry & Cell Biology Volume: 42 Pages: 1416-1429
  • Peer Reviewed
• [Journal Article] Significance of the YLDL motif in the M protein and Alix/AIP1 for Sendai virus budding in the context of virus infection. (2010)
  • Author(s): Irie, T., et al.
  • Journal Title: Virology Volume: 405 Pages: 334-341
  • Peer Reviewed
• [Presentation] センダイウイルスC蛋白質によるウイルスRNA合成の極性制御 (2012)
  • Author(s): 入江崇、坂口剛正
  • Organizer: First Negative Strand Virus-Japan Symposium
  • Place of Presentation: 佐世保市
  • Year and Date: 2012-01-21
• [Presentation] ヌクレオカプシド構造及びIFN誘導性におけるセンダイウイルスC蛋白質の関与 (2012)
  • Author(s): 吉田明日香、坂口剛正、入江崇
  • Organizer: First NegativeStrand Virus-Japan Symposium
  • Place of Presentation: 佐世保市
  • Year and Date: 2012-01-21
• [Presentation] ヌクレオカプシド構造及びIFN誘導性におけるセンダイウイルスC蛋白質の関与 (2012)
  • Author(s): 吉田明日香, 他
  • Organizer: First Negative strand Virus-Japan Symposium
  • Place of Presentation: 佐世保市
  • Year and Date: 2012-01-20
• [Presentation] Sendai virus C protein regulates genomic and antigenomic RNA synthesis during the course of infection (2012)
  • Author(s): Irie, T., Sakaguchi, T
  • Organizer: XVInternational Congress of Virology
  • Place of Presentation: Sapporo
• [Presentation] Sendai virus C protein regulates genomic and antigenomic RNA synthesis during the course of infection (2011)
  • Author(s): Irie, T., et al
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-11
• [Presentation] The accessory C protein of Sendai virus is involved in folding of the N protein (2011)
  • Author(s): Yoshida, A., et al
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-11
• [Presentation] Theaccessory C protein of Sendai virus is involved in folding of the N protein (2011)
  • Author(s): Yoshida, A., Sakaguchi, T., Irie, T
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Sapporo
• [Presentation] センダイウイルス感染におけるアクセサリーV蛋白質の機能 (2011)
  • Author(s): 入江崇、五十嵐友季、坂口剛正
  • Organizer: 第26回中国四国ウイルス研究会
  • Place of Presentation: 徳島市
• [Presentation] アクセサリー蛋白質:パラミクソウイルスの増殖と病原性 (2010)
  • Author(s): 坂口剛正
  • Organizer: 第25回中国四国ウイルス研究会
  • Place of Presentation: 岡山市(招待講演)
  • Year and Date: 2010-06-26
• [Presentation] パラミクソウイルスV蛋白質による自然免疫誘導抑制機構の解明(2) (2010)
  • Author(s): 入江崇、五十嵐友季、吉田明日香、川端涼子、坂口剛正
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島市
• [Presentation] Paramyxovirus Sendai virus V protein binds IRF3 and inhibits its activation (2010)
  • Author(s): Irie, T., Igarashi, T., Yoshida, A., Kawabata, R., Sakaguchi, T
  • Organizer: The10th Awaji international forum on Infection and Immunity
  • Place of Presentation: Awaji
• [Presentation] アクセサリー蛋白質:パラミクソウイルスの増殖と病原性 (2010)
  • Author(s): 坂口剛正
  • Organizer: 第25回中国四国ウイルス研究会
  • Place of Presentation: 岡山市
• [Presentation] センダイウイルスY蛋白質の核移行とインターフェロン応答阻害能の関係 (2010)
  • Author(s): 入江崇、長田奈津子、坂口剛正
  • Organizer: 第25回中国四国ウイルス研究会
  • Place of Presentation: 岡山市
• [Presentation] 杉浦奨励賞受賞講演:エンベロープウイルスの粒子形成・出芽機構の解析 (2009)
  • Author(s): 入江崇
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京、都市センターホテル
  • Year and Date: 2009-10-25
• [Presentation] センダイウイルスY蛋白質の核移行とインターフェロン応答阻害能の関係 (2009)
  • Author(s): 入江崇
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京、都市センターホテル
  • Year and Date: 2009-10-25
• [Presentation] パラミクソウイルスV蛋白質による自然免疫誘導抑制機構の解明 (2009)
  • Author(s): 五十嵐友季、清谷克寛、坂口剛正、入江崇
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京都
• [Presentation] センダイウイルスY蛋白質の核移行とインターフェロン応答阻害能の関係 (2009)
  • Author(s): 入江崇、長田奈津子、坂口剛正
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京都
• [Presentation] アクセサリー蛋白質VとCのセンダイウイルス増殖に及ぼす影響 (2009)
  • Author(s): 加藤篤、永田志保、清谷克寛、久保田耐、坂口剛正
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京都
• [Presentation] センダイウイルスC蛋白質による自然免疫回避機構の多様性 (2009)
  • Author(s): 入江崇、坂口剛正
  • Organizer: 第24回中国四国ウイルス研究会
  • Place of Presentation: 岡山市
• [Remarks]
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