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The use of novel humanized mouse model for the study of immunotherapy against HTLV-1 infection

Research Project

Project/Area Number 21590512
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionUniversity of the Ryukyus

Principal Investigator

SAITO Mineki  琉球大学, 大学院・医学研究科, 准教授 (40398285)

Co-Investigator(Kenkyū-buntansha) TANAKA Yuetsu  琉球大学, 大学院・医学研究科, 教授 (30163588)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords感染防御 / ワクチン / ヒト化マウス / HTLV-1 / 免疫学 / HAM
Research Abstract

In this study, HTLV-1-negative normal human peripheral blood mononuclear cells(PBMCs) were transplanted directly into the spleens of NOD/SCID/γCnull(NOG) mice together with mitomycin treated HTLV-1-infected cell line. We demonstrated that the both human CD4 and CD8 positive T cells were infected with HTLV-1, and that Tax expression was induced by short time(6hrs) in vitro cultivation, as observed in naturally infected T-cells. Moreover, viral gene expression was also very similar to those observed in naturally infected T-cells. Our method provides a simple, rapid and effective approach for assessment of HTLV-1 infection in vivo ; it is therefore useful in the study of infection, pathogenesis and immunotherapies.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (32 results)

All 2012 2011 2010 2009

All Journal Article (13 results) (of which Peer Reviewed: 11 results) Presentation (18 results) Book (1 results)

  • [Journal Article] Immunopathogenesis of Human T-cell leukemia virus type 1(HTLV-1)-associated myelopathy/tropical spastic paraparesis(HAM/TSP): Recent perspectives2012

    • Author(s)
      Saito M, Bangham CR.
    • Journal Title

      Leukemia Research and Treatment

      Volume: 2012 Pages: 1-1

    • DOI

      10.1155/2012/123637

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of an unique CXCR4 epitope whose ligation inhibits infection by both CXCR4 and CCR5 tropic human immunodeficiency type-I viruses2012

    • Author(s)
      Adachi T, Tanaka R, Kodama A, Saito M, Takahashi Y, Ansari AA, Tanaka Y.
    • Journal Title

      Retro virology

      Volume: 8 Pages: 84-84

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of an unique CXCR4 epitope whose ligation inhibits infection by both CXCR4 and CCR5 tropic human immunodeficiency type-I viruses2012

    • Author(s)
      Adachi T, Tanaka R, Kodama A, Saito M, et al
    • Journal Title

      Retrovirology

      Volume: 8 Issue: 1

    • DOI

      10.1186/1742-4690-8-84

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunopathogenesis of Human T-cell leukemia virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) : Recent perspectives2012

    • Author(s)
      Saito M, Bangham CR
    • Journal Title

      Leukemia Research and Treatment

      Volume: Article ID Pages: 259045-259045

    • DOI

      10.1155/2012/259045

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunogenetics and the pathological mechanisms of human T-cell leukemia virus type 1-(HTLV-1)-associated myelopathy/tropical spastic paraparesis(HAM/TSP)2010

    • Author(s)
      Saito M.
    • Journal Title

      Interdisciplinary Perspectives on Infectious Diseases

      Volume: 2010 Pages: 1-8

    • DOI

      10.1155/2010/478461

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Activation of the PI3K-Akt pathway by human T cell leukemia virus type 1(HTLV-1) oncoprotein Tax increases Bcl3 expression, which is associated with enhanced growth of HTLV-1-infected T cells2010

    • Author(s)
      Saito K, Saito M, Taniura N, Okuwa T, Ohara Y.
    • Journal Title

      Virology

      Volume: 403 Pages: 173-180

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] HAM/TSP病態研究の最近の進歩2010

    • Author(s)
      齊藤峰輝
    • Journal Title

      血液・腫瘍科

      Volume: 60 Pages: 642-650

    • NAID

      40019064467

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Journal Article] Activation of the PI3K-Akt pathway by human T cell leukemia virus type 1 (HTLV-1) oncoprotein Tax increases Bc13 expression, which is associated with enhanced growth of HTLV-1-infected T cells.2010

    • Author(s)
      Saito K, Saito M, et al.
    • Journal Title

      Virology

      Volume: 403 Pages: 173-180

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunogenetics and the pathological mechanisms of human T-cell leukemia virus type 1-(HTLV-1)-associated myelopathy/tropical spastic paraparesis(HAM/TSP)2010

    • Author(s)
      齊藤峰輝
    • Journal Title

      Interdisciplinary Perspectives on Infectious Diseases Article ID 478461

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo expression of the HBZ gene of HTLV-1 correlates with proviral load, inflammatory markers and disease severity in HTLV-1 associated myelopathy/tropical spastic paraparesis(HAM/TSP)2009

    • Author(s)
      Saito M, Matsuzaki T, Satou Y, Yasunaga J, Saito K, Arimura K, Matsuoka M, Ohara Y.
    • Journal Title

      Retro virology

      Volume: 6 Pages: 19-19

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] HAM/TSPの病態に関する最近の考え方2009

    • Author(s)
      齊藤峰輝
    • Journal Title

      血液・腫瘍科

      Volume: 56 Pages: 600-606

    • NAID

      40019064284

    • Related Report
      2011 Final Research Report
  • [Journal Article] Immunogenetics of human T-cell leukemia virus type 1(HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP)2009

    • Author(s)
      齊藤峰輝
    • Journal Title

      Inflammation and Regeneration 29

      Pages: 310-316

    • NAID

      10026094043

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HAM/TSPの病態に関する最近の考え方2009

    • Author(s)
      齊藤峰輝
    • Journal Title

      血液・腫瘍科 58

      Pages: 600-606

    • NAID

      40019064284

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] ヒトリンパ球移植免疫不全マウス(hu-PBL-SCID)を用いた新規HTLV-1感染動物モデル作製の試み2011

    • Author(s)
      齊藤峰輝、田中礼子、児玉晃、田中勇悦
    • Organizer
      第4回HTLV-1研究会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-19
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒトリンパ球移植免疫不全マウス(hu-PBL-SCID)を用いた新規HTLV-1感染動物モデル作製の試み2011

    • Author(s)
      齋藤峰輝
    • Organizer
      第4回HTLV-1研究会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2011-09-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] HTLV-1関連脊髄症(HAM)におけるHBZ遺伝子発現の意義2011

    • Author(s)
      齊藤峰輝、田中礼子、児玉晃、松崎敏男、田中勇悦
    • Organizer
      第23回日本神経免疫学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] HTLV-1関連脊髄症(HAM)におけるHBZ遺伝子発現の意義2011

    • Author(s)
      齋藤峰輝
    • Organizer
      第23回日本神経免疫学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] HTLV-1関連脊髄症(HAM)におけるOX40陽性細胞の解析とHTLV-1感染ヒト化マウス作製の試み2011

    • Author(s)
      齊藤峰輝、田中礼子、児玉晃、田中勇悦
    • Organizer
      第64回日本細菌学会九州支部総会・第48回日本ウイルス学会九州支部総会
    • Place of Presentation
      北九州市
    • Year and Date
      2011-08-26
    • Related Report
      2011 Final Research Report
  • [Presentation] HTLV-1関連脊髄症(HAM)におけるOX40陽性細胞の解析とHTLV-1感染ヒト化マウス作製の試み2011

    • Author(s)
      齋藤峰輝
    • Organizer
      第64回日本細菌学会九州支部総会・第48回日本ウイルス学会九州支部総会
    • Place of Presentation
      門司港ホテル(北九州市)
    • Year and Date
      2011-08-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Successful development of novel monoclonal antibodies against HTLV-1 bZIP factor and their applications in studying the pathogenesis of HAM/TSP2011

    • Author(s)
      Mineki Saito
    • Organizer
      15th International Conference on Human Retroviruses : HTLV and Related Viruses
    • Place of Presentation
      Leuven, Belgium
    • Year and Date
      2011-06-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] HTLV-1マイナス鎖にコードされるHBZのHTLV-1関連脊髄症における病因的意義2011

    • Author(s)
      齋藤峰輝
    • Organizer
      第52回日本神経学会学術大会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      2011-05-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] HTLV-1関連脊髄症におけるOX40陽性T細胞の意義2010

    • Author(s)
      齊藤峰輝
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      あわぎんホール(徳島市)
    • Year and Date
      2010-11-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Enhanced expression of OX40 by HTLV-1 Tax and its roles in the pathogenesis of HTLV-1-associated myelopathy/tropical spastic paraparesis(HAM/TSP)2010

    • Author(s)
      Saito M, Tanaka R, Matsuzaki T, Umehara F, Tanaka Y
    • Organizer
      The 10th International Symposium on Neuro Virology
    • Place of Presentation
      Milan, Italy
    • Year and Date
      2010-10-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Enhanced expression of OX40 by HTLV-1 Tax and its roles in the pathogenesis of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP)2010

    • Author(s)
      Mineki Saito
    • Organizer
      The 10th International Symposium on NeuroVirology
    • Place of Presentation
      University of Milan, Italy.
    • Year and Date
      2010-10-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] HTLV-1 bZIP Factor(HBZ)に対する抗体作製とHAM臨床検体を用いた解析2010

    • Author(s)
      齊藤峰輝
    • Organizer
      第3回HTLV-1研究会・合同班会議
    • Place of Presentation
      東京大学医科学研究所(東京都)
    • Year and Date
      2010-08-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Successful development of novel monoclonal antibodies against HTLV-1 bZIP factor and their applications in studying the pathogenesis of HAM/TSP2010

    • Author(s)
      Saito M, Tanaka R, Kodama A, Matsuzaki T, Suehara M, Tanaka Y
    • Organizer
      15th International Conference on Human Retroviruses : HTLV and Related Viruses
    • Place of Presentation
      Leuven, Belgium
    • Year and Date
      2010-06-06
    • Related Report
      2011 Final Research Report
  • [Presentation] HTLV-1関連脊髄症におけるOX40陽性T細胞の意義2010

    • Author(s)
      齊藤峰輝
    • Organizer
      第51回日本神経学会総会
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Year and Date
      2010-05-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] HTLV-1関連脊髄症(HAM/TSP)におけるOX40陽性T細胞の意義2009

    • Author(s)
      齊藤峰輝
    • Organizer
      第57回日本ウイルス学会学術集会
    • Place of Presentation
      都市センターホテル(東京都)
    • Year and Date
      2009-10-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] HTLV-1関連脊髄症(HAM)におけるOX40陽性T細胞の意義2009

    • Author(s)
      齊藤峰輝
    • Organizer
      第2回HTLV-1研究会・合同班会議
    • Place of Presentation
      東京大学医科学研究所(東京都)
    • Year and Date
      2009-08-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] シンポジウム「ATLとHTLV-1研究の最前線」HAM/TSPの病態2009

    • Author(s)
      齊藤峰輝
    • Organizer
      第49回日本リンパ網内系学会総会
    • Place of Presentation
      仙台国際センター(宮城県)
    • Year and Date
      2009-07-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] HAM, ATL治療標的候補分子Bc1-3のHTLV-1感染による高発現機構の解析と制御法の検討2009

    • Author(s)
      齊藤峰輝
    • Organizer
      第50回日本神経学会総会
    • Place of Presentation
      仙台国際センター(宮城県)
    • Year and Date
      2009-05-21
    • Related Report
      2009 Annual Research Report
  • [Book] HTLV-1. Encyclopedia of Genetics 2nd Edition2012

    • Author(s)
      Saito M.
    • Publisher
      Elsevier
    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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