Mechanism of SARS coronavirus infection and proliferation
| Project/Area Number |
21590517
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所) (2011)
Miyagi Cancer Center Research Institute (2009-2010) |
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Principal Investigator |
TANAKA Nobuyuki 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 部長 (60280872)
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| Co-Investigator(Kenkyū-buntansha) |
SUGAMURA Kazuo 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), 発がん制御研究部, 特任部長 (20117360)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | SARS / ウイルス / 共受容体 / 細胞内侵入 / コロナウイルス / ACE2 / 偽ウイルス / スパイク |
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| Research Abstract |
SARS-CoV attaches to the cell surface ACE2, however, requirement of additional cell surface proteins need to be clarified. In this study, we investigated the nature of co-receptors of SARS-CoV. By utilizing soluble SARS-CoV spike protein and a chemical crosslinker reagent, we identified two L-type lectins, DC-SIGN and L-SIGN, in the Spike-ACE2 complex. ACE2 forms complex with these proteins prior to the addition of the Spike. Although neither DC-SIGN nor L-SIGN increased SARS-CoV pseudovirus adsorption to the cell surface, these lectins facilitated incorporation of SARS-CoV in to the cells. Intracytoplasmic region of DC-SIGN and L-SIGN, but not that of ACE2, was required for the incorporation. These results suggest that the intracytoplasmic regions of DC-SIGN and L-SIGN play roles on SARS-CoV infection.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Regulation of hepatitis C virus secretion by the Hrs-dependent exosomal pathway2012
Author(s)
Tamai K, Shiina M, Tanaka N, Nakano T, Yamamoto A, Kondo Y, Kakazu E, Inoue J, Fukushima K, Sano K, Ueno Y, Shimosegawa T, Sugamura K.
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Journal Title
Virology
Volume: 422(2)
Pages: 377-85
Related Report
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[Journal Article] The AAA-ATPase VPS4 regulates extracellular secretion and lysosomal targeting ofα-synuclein2011
Author(s)
Hasegawa T, Konno M, Baba T, Sugeno N, Kikuchi A, Kobayashi M, Miura E, Tanaka N, Tamai K, Furukawa K, Arai H, Mori F, Wakabayashi K, Aoki M, Itoyama Y, Takeda A.
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Journal Title
PLoS One
Volume: 6(12)
Pages: 29460-29460
Related Report
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[Journal Article] Lymphotropic HCV strain can infect human primary naive CD4+cells and affect their proliferation and IFN-γsecretion activity2011
Author(s)
Kondo Y, Ueno Y, Kakazu E, Kobayashi K, Shiina M, Tamai K, Machida K, Inoue J, Wakui Y, Fukushima K, Obara N, Kimura O, Shimosegawa T.
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Journal Title
J Gastroenterol
Volume: 46(2)
Pages: 232-41
Related Report
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[Journal Article] Identification of a high incidence region for retroviral vector integration near exon 1 of the LMO2 locus2009
Author(s)
Yamada K, Tsukahara T, Yoshino K, Kojima K, Agawa H, Yamashita Y, Amano Y, Hatta M, Matsuzaki Y, Kurotori N, Wakui K, Fukushima Y, Osada R, Shiozawa T, Sakashita K, Koike K, Kumaki S, Tanaka N, Takeshita T.
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Journal Title
NAID
Related Report
Peer Reviewed
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[Journal Article] Identification of a high incidence region for retroviral vector inte gration near exon 1 of the LMO2 locus2009
Author(s)
Yamada.K, Tsukahara.T, Yoshino.K, Kojima.K, AgawaH, Yamashita Y, Amano Y, Hatta.M, Matsuzaki.Y, Kurotori N, Wakui K, Fukushima Y, Osada R, Shiozawa T, Sakashita K, Koike K, Kumaki S, Tanaka N, Takeshita T.
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Journal Title
Retrovirology 6巻
Pages: 1-9
Related Report
Peer Reviewed
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