| Project/Area Number |
21590517
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所) (2011)
Miyagi Cancer Center Research Institute (2009-2010) |
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Principal Investigator |
TANAKA Nobuyuki 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 部長 (60280872)
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| Co-Investigator(Kenkyū-buntansha) |
SUGAMURA Kazuo 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), 発がん制御研究部, 特任部長 (20117360)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | SARS / ウイルス / 共受容体 / 細胞内侵入 / コロナウイルス / ACE2 / 偽ウイルス / スパイク |
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| Research Abstract |
SARS-CoV attaches to the cell surface ACE2, however, requirement of additional cell surface proteins need to be clarified. In this study, we investigated the nature of co-receptors of SARS-CoV. By utilizing soluble SARS-CoV spike protein and a chemical crosslinker reagent, we identified two L-type lectins, DC-SIGN and L-SIGN, in the Spike-ACE2 complex. ACE2 forms complex with these proteins prior to the addition of the Spike. Although neither DC-SIGN nor L-SIGN increased SARS-CoV pseudovirus adsorption to the cell surface, these lectins facilitated incorporation of SARS-CoV in to the cells. Intracytoplasmic region of DC-SIGN and L-SIGN, but not that of ACE2, was required for the incorporation. These results suggest that the intracytoplasmic regions of DC-SIGN and L-SIGN play roles on SARS-CoV infection.
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