| Project/Area Number |
21590520
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Virology
|
|---|
| Research Institution | National Institute of Infectious Diseases |
|---|
Principal Investigator |
KATANO Harutaka 国立感染症研究所, 感染病理部, 室長 (70321867)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SATA Tetsutaro 国立感染症研究所, 感染病理部, 部長 (00162397)
SATO Yuko 国立感染症研究所, 感染病理部, 主任研究官
FUKUMOTO Hitomi 国立感染症研究所, 感染病理部, 協力研究員
KANNO Akayuki 国立感染症研究所, 感染病理部, 主任研究官 (50272563)
NAKAMURA Tomoyuki 国立感染症研究所, 感染病理部, 研究生
SAKAMOTO Kouta 国立感染症研究所, 感染病理部, 研究生
TAKENOUCHI Erika 国立感染症研究所, 感染病理部, 研究生
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 病原性 / ポリオーマウイルス / メルケル細胞癌 / ウイルス発癌 / メルケル細胞ポリオーマウイルス / large T抗原 / カポジ肉腫 |
|---|
| Research Abstract |
Merkel cell polyomavirus-DNA was detected in 6 of 11(55%) Japanese cases of Merkel cell carcinoma by real-time PCR. Immunohistochemistry using a rabbit polyclonal antibody revealed that large T antigen was expressed in the nuclei of Merkel cell carcinoma cells. Deletion mutant analyses identified a nuclear localization signal in large T antigen. Real-time PCR analysis revealed that Merkel cell polyomavirus replication is associated with host immunity, and that circulation of human polyomavirus-6 and-7 in the serum is rare.
|
