Mechanism of host chromosome binding of latently infected Epstein-Barr virus episomes

• Project/Area Number: 21590523
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: KANDA Teru 愛知県がんセンター(研究所), 腫瘍ウイルス学部, 室長 (50333472)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: EBウイルス / 潜伏感染 / EBNA1 / リンパ芽球様細胞株 / 免疫沈降 / 質量分析 / エピトープタグ

Research Abstract

Latently-infected Epstein-Barr virus(EBV) genomes are stably maintained by attaching to host chromosomes via virally-encoded EBNA1(Epstein-Barr virus Nuclear Antigen 1) protein. We examined whether cellular p32(TAP, gC1q-R) protein, a major binding partner of EBNA1, plays any role in the chromosome binding of EBNA1.Suppression of p32 expression in EBV infected cells did not affect the chromosomal localization of EBNA1.The result argues against the possibility that p32 protein has any role in the chromosome binding of EBNA1.

Research Products

• [Journal Article] Unexpected instability of Family of Repeats(FR), the critical cis-acting sequence required for EBV latent infection, in EBV-BAC systems (2011)
  • Author(s): Kanda T(corresponding author), Shibata S, Saito S, Murata T, Isomura H, Yoshiyama H, Takada K, and Tsurumi T
  • Journal Title: PLoS One Volume: 6(11)
  • Peer Reviewed
• [Journal Article] A DNA ligand designed to antagonize EBNA1 represses Epstein-Barr virus-induced immortalization (2011)
  • Author(s): Yasuda A., Noguchi K., Minoshima M., Kashiwazaki G., Kanda T., Katayama K., Mitsuhashi J., Bando T., Sugiyama H., Sugimoto Y
  • Journal Title: Cancer Sci Volume: 102(12) Issue: 12 Pages: 2221-2230
  • DOI: 10.1111/j.1349-7006.2011.02098.x
  • NAID: 10030246127
  • Peer Reviewed
• [Journal Article] Unexpected Instability of Family of Repeats (FR), the Critical cis-Acting Sequence Required for EBV Latent Infection, in EBV-BAC Systems (2011)
  • Author(s): Teru Kanda
  • Journal Title: PLoS One Volume: 6 Issue: 11 Pages: e27758-e27758
  • DOI: 10.1371/journal.pone.0027758
  • Peer Reviewed
• [Journal Article] Distinctive effects of the Epstein-Barr virus family of repeats on viral latent gene promoter activity and B-lymphocyte transformation (2009)
  • Author(s): Ali A. K. M., Saito S., Shibata S., Takada K., and Kanda T.
  • Journal Title: J Virol. Volume: 83(18) Issue: 18 Pages: 9163-9174
  • DOI: 10.1128/jvi.01979-08
  • Peer Reviewed
• [Journal Article] Distinctive Effects of the Epstein-Barr Virus Family of Repeats on Viral Latent Gene Promoter Activity and B-lymphocyte Transformation (2009)
  • Author(s): Ahmed KM Ali
  • Journal Title: Journal of Virology 83 Pages: 9163-9174
  • Peer Reviewed
• [Journal Article] HLA-restricted presentation of WT1 tumor antigen in B-lymphoblastoid cell lines established using a maxi-EBV system
  • Author(s): Kanda T(corresponding author), Ochi T, Fujiwara H, Yasukawa M, Okamoto S, Mineno J, Kuzushima K, and Tsurumi T
  • Journal Title: Cancer Gene Ther
  • Peer Reviewed
• [Presentation] FR (family of repeats)1次配列のEBウイルス株間における多様性とその意義 (2011)
  • Author(s): 神田輝
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2011-10-05
• [Presentation] FR(family of repeats) 1次配列のEBウイルス株間における多様性とその意義 (2011)
  • Author(s): 神田輝、村田貴之、高田賢蔵、鶴見達也
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋市
• [Presentation] Primary sequence heterogeneity of Family of Repeats(FR) of Epstein-Barr virus(EBV) results in strain-specific differences in the FR stability in BAC vectors (2011)
  • Author(s): 神田輝、村田貴之、高田賢蔵、鶴見達也
  • Organizer: International Union of Microbiological Societies(IUMS) 2011, XV International Congress of Virology
  • Place of Presentation: 札幌
• [Presentation] Primary sequence heterogeneity of Family of Repeats(FR) of Epstein-Barr virus(EBV) results in strain-specific differences in the FR stability in BAC vectors (2011)
  • Author(s): 神田輝、村田貴之、高田賢蔵、鶴見達也
  • Organizer: 5th International Symposium on Nasopharyngeal Carcinoma
  • Place of Presentation: マレーシアPenang
• [Presentation] EBウイルス潜伏感染細胞内におけるEBNA1蛋白質結合細胞性因子の解析 (2010)
  • Author(s): 神田輝
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島)
  • Year and Date: 2010-11-08
• [Presentation] Family of repeats(FR) 1次配列のEBV株間における多様性と共通点について (2010)
  • Author(s): 神田輝、鶴見達也
  • Organizer: 第8回EBウイルス研究会
  • Place of Presentation: 大阪市
• [Presentation] EBウイルス潜伏感染細胞内におけるEBNA1蛋白質結合細胞性因子の解析 (2010)
  • Author(s): 神田輝、鶴見達也
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島市
• [Presentation] The impact of viral repetitive sequences on the biological properties of Epstein-Barr virus recombinants (2010)
  • Author(s): 神田輝、鶴見達也
  • Organizer: 14th Biennial Conference of the International Association for Research on Epstein-Barr Virus and Associated Diseases
  • Place of Presentation: 英国Birmingham
• [Presentation] 潜伏感染細胞中でEBNA1蛋白質と相互作用する細胞性因子の解析 (2010)
  • Author(s): 神田輝、鶴見達也
  • Organizer: 第7回EBウイルス研究会、第7回EBウイルス研究会
  • Place of Presentation: 札幌市
• [Presentation] 潜伏感染EBウイルスエピゾームの宿主染色体付着機構の解析 (2010)
  • Author(s): 神田輝、鶴見達也
  • Organizer: 第25回ヘルペスウイルス研究会,第25回ヘルペスウイルス研究会
  • Place of Presentation: 浜松市
• [Presentation] 潜伏感染EBウイルスエピゾームの宿主染色体付着に必要なシス塩基配列の解析 (2010)
  • Author(s): 神田輝
  • Organizer: 第27回染色体ワークショップ
  • Place of Presentation: 御殿場市
• [Presentation] 純粋な組換えEBVを用いたエピトープタグ付加EBNA1蛋白質発現リンパ芽球様細胞株の樹立 (2009)
  • Author(s): 神田輝
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル(東京)
  • Year and Date: 2009-10-27
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/ri/01bumon/06shuyo_uirusu/index.html
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/ri/01bumon/06shuyo_uirusu/index.html