Study of the roles of innate immunity on acute and chronic hepatitis C by a novel non-human primate model
| Project/Area Number |
21590526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Tomoyuki 京都大学, 霊長類研究所, 特定助教 (00451948)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 個体 / C型肝炎 / 動物モデル / 自然免疫 / HCV / GBV-B / NK細胞 / CD16 / 新世界ザル / CD16(3G8) / マーモセット / 霊長類モデル |
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| Research Abstract |
We aimed to clarify the role of innate immunity during acute and chronic hepatitis C by a novel non-human primate model. First of all, we established a quantification system of NK cell activity of tamarins. We then found that the intravenous administration of an anti-CD16 monoclonal antibody 3G8 at a dose of 50mg/kg to tamarins successfully depleted CD16+ NK cells, which was consistent with the reduction of NK cell-mediated cytotoxicity. It is notable that lowered but detectable levels of NK activity were observed in the blood obtained from the CD16+ NK cell-depleted tamarins, indicating the presence of a minor CD16-NK subpopulation. By using these assay systems we established, analyses of the roles of the CD16+ NK cells on dynamics of the replication of GBV-B, which is closely related to hepatitis C virus and is classified in genus hepachivirus, and antiviral acquired immune responses in the virus-infected tamarins are now ongoing. On the basis of the results obtained, we would like to examine in detail regarding the roles of NK cells on the control of persistency of the hepatitis virus in near future.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] CD16 positive natural killer cell splay a limited role against primary dengue virus infection in tamarins2012
Author(s)
Yoshida T, Omatsu T, Saito A, Katakai Y, Iwasaki Y, Kurosawa T, Hamano M, Nakamura S, Takasaki T, Yasutomi Y, Kurane I, Akari H
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Journal Title
Archives of Virology
Volume: 157
Issue: 2
Pages: 363-368
DOI
Related Report
Peer Reviewed
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[Journal Article] Long-term persistent GBV-B infection and development of a progressive chronic hepatitis C-like disease in marmosets2011
Author(s)
Iwasaki Y, Mori K, Ishii K, Maki N, Iijima S, Yoshida T, Okabayashi S, Katakai Y, Lee Y-J, Saito A, Fukai H, Kimura N, Ageyama N, Yoshizaki S, Suzuki T, Yasutomi Y, Miyamura T, Kannagi M, Akari H
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Journal Title
Frontiers in Microbiology
Volume: 2
Pages: 240-240
DOI
Related Report
Peer Reviewed
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[Presentation] Long-termpersistent GBV-B infection and development of a progressive chronic hepatitis C-like disease in marmosets.2011
Author(s)
Hirofumi Akari, Yuki Iwasaki, Ken-ichi Mori, Koji Ishii, Noboru Maki, Sayuki Iijima, Tomoyuki Yoshida, Sachi Okabayashi, Yuko Katakai, Young-Jung Lee, Akatsuki Saito
Organizer
International Union of Microbiological Society 2011 Congress
Place of Presentation
Sapporo
Related Report
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[Presentation] Selective and frequent on-synonymous mutations of the viral genome in chronically GBV-B-infected marmosets.2009
Author(s)
Yoshida T, Iwasaki Y, Mori K, Maki N, Ishii K, Iijima S, Yoshizaki S, Katakai Y, Suzuki T, Miyamura T, Akari H
Organizer
16^<th> International Symposium on Hepatitis C Virus and Related Viruses
Place of Presentation
France, Nice
Related Report
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