| Project/Area Number |
21590526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Tomoyuki 京都大学, 霊長類研究所, 特定助教 (00451948)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 個体 / C型肝炎 / 動物モデル / 自然免疫 / HCV / GBV-B / NK細胞 / CD16 / 新世界ザル / CD16(3G8) / マーモセット / 霊長類モデル |
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| Research Abstract |
We aimed to clarify the role of innate immunity during acute and chronic hepatitis C by a novel non-human primate model. First of all, we established a quantification system of NK cell activity of tamarins. We then found that the intravenous administration of an anti-CD16 monoclonal antibody 3G8 at a dose of 50mg/kg to tamarins successfully depleted CD16+ NK cells, which was consistent with the reduction of NK cell-mediated cytotoxicity. It is notable that lowered but detectable levels of NK activity were observed in the blood obtained from the CD16+ NK cell-depleted tamarins, indicating the presence of a minor CD16-NK subpopulation. By using these assay systems we established, analyses of the roles of the CD16+ NK cells on dynamics of the replication of GBV-B, which is closely related to hepatitis C virus and is classified in genus hepachivirus, and antiviral acquired immune responses in the virus-infected tamarins are now ongoing. On the basis of the results obtained, we would like to examine in detail regarding the roles of NK cells on the control of persistency of the hepatitis virus in near future.
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