Identification of ubiquitin-dependent endosomal sorting signal in cytokine receptors

• Project/Area Number: 21590530
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Shinshu University
• Principal Investigator: TAKESHITA Toshikazu 信州大学, 医学部, 教授 (60212023)
• Co-Investigator(Renkei-kenkyūsha): KOJIMA Katsuhiko 信州大学, 医学部, 助教 (80345743) ; YOSHINO Kazuhisa 信州大学, 医学部, 助教 (40551859) ; AMANO Yuji 信州大学, 医学部, 助教 (50624681)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 小胞輸送 / サイトカイン受容体 / エンドソーム / ユビキチン / 小胞輸送シグナル

Research Abstract

In immune or nerve system, intracellular compartments maintain the function, which is supported by endosomal sorting, keeps the specific substances of the system and exchanges the substances between the compartments without physiological and morphological disorder. Here, we showed ubiquitin-independent endosomal sorting of interleukin-2 receptorβ(IL-2Rβ) and identified a novel endosomal sorting signal in the cytoplasmic region of IL-2Rβ. Our report provides the first description of endosomal sorting signal in a ubiquitin-independent manner.

Research Products

• [Journal Article] Hrs recognizes a hydrophobic amino acid cluster in cytokine receptors during ubiquitin-independent endosomal sorting (2011)
  • Author(s): Amano Y, Yamashita Y, Tanaka N, Sugamura K, 他
  • Journal Title: J Biol Chem Volume: 286 Issue: 17 Pages: 15458-15472
  • DOI: 10.1074/jbc.m110.191924
  • NAID: 120007110518
  • Peer Reviewed
• [Presentation] Hrs recognizes a hydrophobic amino acid cluster in cytokine receptors during ubiquitin-independent endosomal sorting (2011)
  • Author(s): 天野勇治、小嶋克彦、吉野和寿、竹下敏一
  • Organizer: 日本免疫学会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-29
• [Presentation] Identification of Hrs-binding motif in the cytokine receptors delivered to lysosome in ubiquitin-independent manner (2010)
  • Author(s): 天野勇治、小嶋克彦、山下勇毅、吉野和寿、竹下敏一
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Identification of Hrs-binding motif in the cytokine receptors delivered to lysosome in ubiquitin-independent manner. (2010)
  • Author(s): 天野勇治
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Identification of Hrs-binding motif in the cytokine receptors delivered to lysosome in ubiquitin-independent manner (2010)
  • Author(s): 天野勇治、小嶋克彦、山下勇毅、吉野和寿、竹下敏一
  • Organizer: 国際免疫学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-24
• [Presentation] Identification of Hrs-binding motif in the cytokine receptors delivered to lysosome in ubiquitin-independent manner. (2010)
  • Author(s): 天野勇治
  • Organizer: 国際免疫学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-24
• [Presentation] 細胞内小胞輸送と細胞増殖におけるIL-2受容体β鎖のHrs結合領域の役割 (2009)
  • Author(s): 山下勇毅、小嶋克彦、天野勇治、吉野和寿、竹下敏一
  • Organizer: 日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-02
• [Presentation] ユビキチン非依存的に小胞輸送されるサイトカイン受容体のHrs会合モチーフの同定 (2009)
  • Author(s): 天野勇治、小嶋克彦、山下勇毅、吉野和寿、竹下敏一
  • Organizer: 日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-02
• [Presentation] ユビキチン非依存性に小胞輸送されるサイトカイン受容体のHrs会合モチーフの同定 (2009)
  • Author(s): 天野勇治
  • Organizer: 日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-02