NECROTIC DNA FRAGMENTATION AND ASEPTIC INFLAMMATION

• Project/Area Number: 21590542
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Tokyo University of Science
• Principal Investigator: MIZUTA Ryushin 東京理科大学, 生命科学研究所, 准教授 (50297628)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ネクローシス / DNA断片化 / 無菌的炎症 / 酸化ストレス

Research Abstract

The DNA fragmentation in cell death is a well-known phenomenon, but its physiological significance has insufficiently investigated. We found the aggravated drug-induced liver injury in the mouse deleted one of the genes of DNA fragmentation proteins. The aggravation is due to the increase of oxidative stress. Thus, the DNA fragmentation in cell death is essential for scavenging dead cells and maintaining homeostasis by reducing oxidative stress.

Research Products

• [Journal Article] 乳汁中HMGB1蛋白量はウシ乳房炎重症度と相関する (2012)
  • Author(s): 水田龍信
  • Journal Title: 日本乳房炎研究会(第16回学術集会)Proceedings Pages: 29-31
• [Journal Article] Increased concentration of high-mobility-group box 1(HMGB1) protein in milk is related to the severity of bovine mastitis (2011)
  • Author(s): Furukawa, Y., Hayashi, T., Mizuta, M., Ebara, S., Kiku, Y., Ozawa, T., Matsubara, T., Ito, I., Kitamura, D., Mizuta, R.
  • Journal Title: Vet Res Commun Volume: 35 Pages: 47-54
• [Journal Article] Increased concentration of high-mobility-group box 1 (HMGB1) protein in milk is related to the severity of bovine mastitis. (2011)
  • Author(s): 古川祐樹
  • Journal Title: Vet Res Commun Volume: 35 Pages: 47-54
  • Peer Reviewed
• [Journal Article] Transcribed Guanine-rich RNA Aggregates on Template DNA and Changes Its Conformation (2010)
  • Author(s): Mizuta, R.
  • Journal Title: Chemistry Letters Volume: 39 Pages: 1088-1089
  • NAID: 10027185890
• [Journal Article] Transcribed Guanine-rich RNA Aggregates on Template DNA and Changes Its Conformation. (2010)
  • Author(s): 水田龍信
  • Journal Title: Chemistry Letter Volume: 39 Pages: 1088-1089
  • NAID: 10027185890
  • Peer Reviewed
• [Journal Article] DNaseγ-dependent and-independent apoptotic DNA fragmentations in Ramos Burkitt's lymphoma cell line (2009)
  • Author(s): Mizuta, R., Mizuta, M., Araki, S., Suzuki, K., Ebara, S., Furukawa, Y., Shiokawa, D., Tanuma, S., Kitamura, D
  • Journal Title: BIOMEDICAL RESEARCH Volume: 30 Pages: 165-170
• [Journal Article] Possible contribution of Dnaseγto immunoglobulin V gene diversification (2009)
  • Author(s): Okamoto, N., Okamoto, M., Araki, S., Arakawa, H., Mizuta, R., Kitamura, D.
  • Journal Title: Immunology Letters Volume: 125 Pages: 22-30
• [Journal Article] DNase γ-dependent and -independent apoptotic DNA fragmentations in Ramos Burkitt's lymphoma cell line (2009)
  • Author(s): 水田龍信
  • Journal Title: BIOMEDICAL RESEARCH 30 Pages: 165-170
  • Peer Reviewed
• [Journal Article] Possible contribution of Dnase γ to immunoglobulin V gene diversification (2009)
  • Author(s): 岡本憲明
  • Journal Title: Immunology Letters 125 Pages: 22-30
  • Peer Reviewed
• [Presentation] DNAによる核の堅牢性、脆弱性の制御-薬剤性肝障害を例として (2012)
  • Author(s): 水田龍信
  • Organizer: 第3回バイオメディカルインターフェース・ワークショップ
  • Place of Presentation: 宮古島公民館(宮古島)
  • Year and Date: 2012-02-25
• [Presentation] DNA断片化と細胞核の分解 (2011)
  • Author(s): 水田龍信、三沢正行、荒井智也、北村大介
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] DNA断片化と細胞核の分解 (2011)
  • Author(s): 水田龍信
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-15
• [Presentation] 乳汁中HMGB1蛋白量はウシ乳房炎重症度と相関する (2011)
  • Author(s): 水田龍信、菊佳男、尾澤知美、松原朋子、林智人
  • Organizer: 第16回日本乳房炎研究会学術集会
  • Place of Presentation: 筑波
  • Year and Date: 2011-10-14
• [Presentation] 乳汁中HMGB1蛋白量はウシ乳房炎重症度と相関する (2011)
  • Author(s): 水田龍信
  • Organizer: 第16回日本乳房炎研究会学術集会
  • Place of Presentation: つくば国際会議場(筑波)
  • Year and Date: 2011-10-14
• [Presentation] 乳汁中HMGB1蛋白量はウシ乳房炎重症度と相関する (2011)
  • Author(s): 水田龍信
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学(大阪)
  • Year and Date: 2011-09-19
• [Presentation] Regulation of oxidative stress and tumorigenesis (2011)
  • Author(s): 水田龍信
  • Organizer: International Symposium on Technologies against Cancer 2011
  • Place of Presentation: Tokyo/Tower Hall Funabori
  • Year and Date: 2011-09-02
• [Presentation] DNAによる核の堅牢性、脆弱性の制御 (2011)
  • Author(s): 水田龍信
  • Organizer: 日本生化学会関東支部例会
  • Place of Presentation: 東京理科大学森戸記念館(東京)(招待講演)
  • Year and Date: 2011-06-25
• [Presentation] 肝細胞死におけるDNA断片化酵素の役割 (2011)
  • Author(s): 水田龍信
  • Organizer: 第18回肝細胞研究会
  • Place of Presentation: 東京ガーデンパレス(東京)
  • Year and Date: 2011-06-24
• [Presentation] アセトアミノフェン誘発性肝障害における生理的機能解析 (2010)
  • Author(s): 荏原翔太
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] DNA断片化と細胞核の分解 (2009)
  • Author(s): 水田龍信
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11