A mechanism analysis for the formation of drug dependence

• Project/Area Number: 21590601
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied pharmacology
• Research Institution: Iwate Medical University
• Principal Investigator: IRIE Yasuyuki 岩手医科大学, 医学部, 准教授 (70303948)
• Co-Investigator(Renkei-kenkyūsha): TAIRA Eiichi 岩手医科大学, 医学部薬理学講座, 教授 (60263240) ; KONDO Yukiko 岩手医科大学, 医学部薬理学講座, 助教 (70347847)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 遺伝子 / 神経科学 / 脳・神経 / 薬理学 / 薬物依存

Research Abstract

This study was initiated for the purpose to explore the possibility to elucidate the molecular mechanism of drug dependence, such as psychostimulants, to treat or prevent this. When Neuro2a cells were transfected by Amida, a component of the chromatin remodeling complex, and its related proteins, it turned out that the proliferation rate and adhesion ability were affected. These results were caused because the expression of genes for specific cellar functions were altered. Meanwhile, it was revealed that cell death through the endoplasmic reticulum stress was induced by methamphetamine treatment of the CATH. a cells, a cell line derive from dopaminergic neurons in central nervous system.

Research Products

• [Journal Article] Methamphetamine induces endoplasmic reticulum stress related gene CHOP/Gadd153/ddit3 in dopaminergic cells (2011)
  • Author(s): Irie, Yasuyuki ; Saeki, Makio ; Tanaka, Hidekazu ; Kanemura, Yonehiro ; Otake, Shinpei ; Ozono, Yoshiyuki ; Nagai, Toshisaburou ; Kondo, Yukiko ; Kudo, Kenzo ; Kamisaki, Yoshinori ; Miki, Naomasa ; Taira, Eiichi
  • Journal Title: Cell and Tissue Research Volume: Vol.345 Pages: 231-241
  • Peer Reviewed
• [Journal Article] Methamphetamine induces endoplasmic reticulum stress related gene CHOP/Gadd153/ddit3 in dopaminergic cells (2011)
  • Author(s): Irie, Yasuyuki; Saeki, Makio; Tanaka, Hidekazu; Kanemura, Yonehiro; Otake, Shinpei; Ozono, Yoshiyuki; Nagai, Toshisaburou; Kondo, Yukiko; Kudo, Kenzo; Kamisaki, Yoshinori; Miki, Naomasa; Taira, Eiichi
  • Journal Title: Cell and Tissue Research Volume: Vol.345:2 Issue: 2 Pages: 231-241
  • DOI: 10.1007/s00441-011-1207-5
  • Peer Reviewed
• [Journal Article] Inflammatory cytokines decrease the expression of nicotinic acetylcholine receptor during the cell maturation (2010)
  • Author(s): Yukiko Kondo, Eiichi Tachikawa, Shinpei Ohtake, Kenzo Kudo, Kenzo Mizuma, Takeshi Kashimoto, Yasuyuki Irie, Eiichi Taira
  • Journal Title: Mol Cell Biochem Volume: 333 Pages: 57-64
  • Peer Reviewed
• [Journal Article] Inflammatory cytokines decrease the expression of nicotinic acetylcholine receptor during the cell maturation. (2010)
  • Author(s): Yukiko Kondo, Eiichi Tachikawa, Shinpei Ohtake, Kenzo Kudo, Kenzo Mizuma, Takeshi Kashim oto, Yasuyuki Irie, Eiichi Taira
  • Journal Title: Mol Cell Biochem. 333 Pages: 57-64
  • Peer Reviewed
• [Presentation] Phenotypic change of vascular smooth muscle cells by cyclic stretch and vasoactive substances effect (2012)
  • Author(s): 近藤ゆき子、入江康至、平英一
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都
• [Presentation] Monad regulates stability of Amphiregulin mRNA (2012)
  • Author(s): 栗岡恭子、佐伯万騎男、入江康至、上崎善規
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都
• [Presentation] Methamphetamine induces endoplasmic reticulum stress related gene CHOP/Gadd153/ddit3 in dopaminergic cells (2012)
  • Author(s): 入江康至、佐伯万騎男、近藤ゆき子、上崎善規、平英一
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都
• [Presentation] Searching a Substrate for Denitrase, an Activity that Reduces Nitrotyrosine Immunoreactivity in Proteins (2011)
  • Author(s): Yasuyuki Irie
  • Organizer: 9th Annual Congress of International Drug Discovery Science and Technology
  • Place of Presentation: Shenzhen
  • Year and Date: 2011-11-05
• [Presentation] Expression Change of Nnicotinic Receptor by the Cell Maturity in the APC Cell Line (2011)
  • Author(s): Yukiko Kondo, Yasuyuki Irie, Eiichi Taira
  • Organizer: 9th Annual Congress of International Drug Discovery Science and Technology
  • Place of Presentation: Shenzhen
  • Year and Date: 2011-11-05
• [Presentation] Invited Lecture Searching a Substrate for Denitrase, an Activity that Reduces Nitrotyrosine Immunoreactivity in Proteins (2011)
  • Author(s): Yasuyuki Irie
  • Organizer: 9th Annual Congress of International Drug Discovery Science and Technology
  • Place of Presentation: Shenzhen
  • Year and Date: 2011-11-05
• [Presentation] 新規成長抑制因子Amidaの機能解析 (2011)
  • Author(s): 入江康至、佐伯万騎男、加藤功一、近藤ゆき子、大竹伸平、水間謙三、岩田博夫、上崎善規、平英一
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] 血管平滑筋細胞の周期性伸展培養と生理活性物質刺激による形質変換 (2011)
  • Author(s): 近藤ゆき子、水間謙三、入江康至、平英一
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] 新規細胞死関連蛋白質Amidaの機能解析 (2010)
  • Author(s): 入江康至
  • Organizer: 岩手医科大学未来医療開発プロジェクトキックオフミティング
  • Place of Presentation: 盛岡
  • Year and Date: 2010-12-11
• [Presentation] Toxic dose of methamphetamine induces endoplasmic reticulum stress related gene CHOP/Gadd153/ddit3 in dopaminergic neural cells (2010)
  • Author(s): Yasuyuki Irie, Makio Saeki, Hidekazu Tanaka, Yonehiro Kanemura, Yoshiyuki Ozono, Toshisaburou Nagai, Yasuhiro Tsukamoto, Hidenori Kato, Yukiko Kondo, Kenzo Kudo, Kenzo Mizuma, Shinpei Otake, Yoshinori Kamisaki, Naomasa Miki, Eiichi Taira
  • Organizer: The 16th World Congress of Basic and Clinical Pharmacology(WorldPharma 2010)
  • Place of Presentation: Copenhagen
  • Year and Date: 2010-07-21
• [Presentation] Toxic dose of methamphetamine induces endoplasmic reticulum stress related gene CHOP/Gadd153/ddit3 in dopaminergic neural cells. (2010)
  • Author(s): Yasuyuki Irie, Makio Saeki, Hidekazu Tanaka, Yonehiro Kanemura, Yoshiyuki Ozono, Toshisaburou Nagai, Yasuhiro Tsukamoto, Hidenori Kato, Yukiko Kondo, Kenzo Kudo, Kenzo Mizuma, Shinpei Otake, Yoshinori Kamisaki, Naomasa Miki, Eiichi Taira
  • Organizer: The 16th World Congress of Basic and Clinical Pharmacology (WorldPharma 2010)
  • Place of Presentation: Copenhagen
  • Year and Date: 2010-07-21
• [Presentation] Expression change in nicotinic receptor according to cellular maturity of antigen presenting cell line (2010)
  • Author(s): Yukiko Kondo, Eiichi Tachikawa, Shinpei Ohtake, Kenzo Kudo, Kenzo Mizuma, Takeshi Kashimoto, Yasuyuki Irie, Eiichi Taira
  • Organizer: The 16th World Congress of Basic and Clinical Pharmacology(WorldPharma 2010)
  • Place of Presentation: Copenhagen
  • Year and Date: 2010-07-20
• [Presentation] Expression change in nicotinic receptor according to cellular maturity of antigen presenting cell line (2010)
  • Author(s): Yukiko Kondo, Eiichi Tachikawa, Shinpei Ohtake, Kenzo Kudo, Kenzo Mizuma, Takeshi Kashimoto, Yasuyuki Irie, Eiichi Taira
  • Organizer: The 16th World Congress of Basic and Clinical Pharmacology (WorldPharma 2010)
  • Place of Presentation: Copenhagen
  • Year and Date: 2010-07-20
• [Presentation] IGF-1によるギセリン発現の制御 (2010)
  • Author(s): 大竹伸平、入江康至、近藤ゆき子、水間謙三、平英一
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
• [Presentation] 血管平滑筋細胞の周期性伸展培養による生理活性物質受容体の発現変化 (2010)
  • Author(s): 近藤ゆき子、大竹伸平、水間謙三、入江康至、平英一
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
• [Presentation] 局所麻酔薬が副腎髄質細胞に及ぼす影響 (2010)
  • Author(s): 水間謙三、近藤ゆき子、入江康至、大竹伸平、平英一、鈴木健二
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
• [Presentation] 新規細胞死関連蛋白質Amidaの機能解析 (2010)
  • Author(s): 入江康至、佐伯万騎男、近藤ゆき子、大竹伸平、水間謙三、上崎善規、平英一
  • Organizer: 第61回日本薬理学会北部会
  • Place of Presentation: 札幌
• [Presentation] 新規細胞死関連蛋白質Amidaの機能解析 (2010)
  • Author(s): 入江康至
  • Organizer: 岩手医科大学未来医療開発プロジェクトキックオフミーティング
  • Place of Presentation: 盛岡
• [Presentation] Methamphetamineは、ドパミン作動性ニューロン由来細胞に小胞体ストレスを引き起こす (2010)
  • Author(s): 入江康至、佐伯万騎男、近藤ゆき子、上崎善規、平英一
  • Organizer: 第62回日本薬理学会北部会
  • Place of Presentation: 仙台
• [Presentation] Methamphetamine induced dopamine terminal toxicity involves endoplasmic reticulum stress (2009)
  • Author(s): Yasuyuki Irie
  • Organizer: International Workshop on Signal Transduction
  • Place of Presentation: Shanghai
  • Year and Date: 2009-07-23
• [Presentation] Methamphetamine induced dopamine terminal toxicity involves endoplasmic reticulum stress. (2009)
  • Author(s): Yasuyuki Irie
  • Organizer: International Workshop on Signal Transduction
  • Place of Presentation: 上海
  • Year and Date: 2009-07-23
• [Presentation] Psychostimulantによって発現調節される遺伝子 (2009)
  • Author(s): 入江康至、佐伯万騎男、近藤ゆき子、大竹伸平、水間謙三、上崎善規、平英一
  • Organizer: 第60回日本薬理学会北部会
  • Place of Presentation: 富山