| Project/Area Number |
21590612
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Toyama |
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Principal Investigator |
NIIMI Hideki 富山大学, 大学病院, 助教 (50401865)
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| Co-Investigator(Kenkyū-buntansha) |
KITAJIMA Isao 富山大学, 大学院・医学薬学研究部(医学), 教授 (50214797)
野手 良剛 富山大学, 大学病院, 主任臨床検査技師 (60377364)
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| Co-Investigator(Renkei-kenkyūsha) |
NOTE Ryougou 富山大学, 大学病院, 主任臨床検査技師 (60377364)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 遺伝子検査学 / 遺伝子 / 微生物 / 遺伝子検査 / 敗血症 / 感染症 |
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| Research Abstract |
To detect pathogenic bacteria by PCR sensitively and correctly, we newly developed "eukaryote-made" Taq polymerase, which is free from bacterial DNA contamination(J Clin Microbiol. 2011 Sep ; 49(9): 3316-20). We also developed a novel rapid method to identify pathogenic microorganisms using 7 primer sets, real-time PCR, high resolution melting analysis(RotorGeneQ : QIAGEN), and the original web-based identification software(International patent application : 2007). To combine the eukaryote-made Taq polymerase and a novel identification method, we developed a rapid identification system of pathogenic microorganisms within 3 hours after collection of patient samples.
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