Development of a novel, rapid, easy and affordable identification IT system for pathogens in sepsis
| Project/Area Number |
21590612
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
NIIMI Hideki 富山大学, 大学病院, 助教 (50401865)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KITAJIMA Isao 富山大学, 大学院・医学薬学研究部(医学), 教授 (50214797)
野手 良剛 富山大学, 大学病院, 主任臨床検査技師 (60377364)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
NOTE Ryougou 富山大学, 大学病院, 主任臨床検査技師 (60377364)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 遺伝子検査学 / 遺伝子 / 微生物 / 遺伝子検査 / 敗血症 / 感染症 |
|---|
| Research Abstract |
To detect pathogenic bacteria by PCR sensitively and correctly, we newly developed "eukaryote-made" Taq polymerase, which is free from bacterial DNA contamination(J Clin Microbiol. 2011 Sep ; 49(9): 3316-20). We also developed a novel rapid method to identify pathogenic microorganisms using 7 primer sets, real-time PCR, high resolution melting analysis(RotorGeneQ : QIAGEN), and the original web-based identification software(International patent application : 2007). To combine the eukaryote-made Taq polymerase and a novel identification method, we developed a rapid identification system of pathogenic microorganisms within 3 hours after collection of patient samples.
|
Report
(4 results)
Research Products
(52 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Huh-7 ; a HFE-mutated hemochromatotic cell line2010
Author(s)
Hideki Niimi, Atsuko Takano, Terumi Takahara, and Isao Kitajima
Organizer
which suggest the mechanism of Hepci din expression regulated by HFE~ BM P6-Smad pathway, TGF-b meeting in Le iden
Place of Presentation
ライデン(オランダ)
Year and Date
2010-09-03
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
