Podocyte injury induced by chronic hypoxia in diabetic db/db mice

• Project/Area Number: 21590617
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: University of Fukui
• Principal Investigator: TAKAHASHI Naoki 福井大学, 医学部・附属病院, 助教 (30377460)
• Co-Investigator(Kenkyū-buntansha): KIMURA Hideki 福井大学, 医学部, 准教授 (20283187) ; KASUNO Kenji 福井大学, 医学部附属病院, 助教 (60455243) ; YOSHIDA Haruyoshi 福井大学, 医学部, 教授 (80135574)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 糖尿病 / 糖尿病性腎症 / 低酸素 / ポドサイト

Research Abstract

We analyzed the glomerular lesion induced by chronic hypoxia in db/db mice. Hypoxia-associated glomerular injury may result from the increased macrophage infiltration via monocyte chemoattractant protein-1(MCP-1), but not from vascular endothelial growth factor-A(VEGF-A).

Research Products

• [Journal Article] Severe intraglomerular detachment of podocytes in a Gitelman syndrome patient (2012)
  • Author(s): Naoki Takahashi, Hideki Kimura, Shinichi Mizuno, Masanori Hara, Yoshiaki Hirayama, Hiroyuki Kurosawa, Shouji Tsutaya, Minoru Yasujima, Seiji Yokoi, Daisuke Mikami, Kenji Kasuno, Hironobu Naiki, Masayuki Iwano and Haruyoshi Yoshida
  • Journal Title: Clin Exp Nephrol Volume: (in press) Issue: 3 Pages: 16-16
  • DOI: 10.1007/s10157-012-0624-4
  • NAID: 10030808799
  • Peer Reviewed
• [Journal Article] 腎障害をきたす全身疾患-最新の進歩III.パラプロテイン血症2.アミロイドーシス (2011)
  • Author(s): 高橋直生, 木村秀樹, 内木宏延, 吉田治義
  • Journal Title: 日本内科学会雑誌 Volume: 100 Pages: 1282-1288
• [Journal Article] Smaller LDL size as a possible risk factor for the prevalence of coronary artery diseases in hemodialysis patients-Associations of CETP and the hepatic lipase gene polymorphism with LDL size (2011)
  • Author(s): Kimura H, Miyazaki R, Imura T, Masunaga S, Shimada A, Mikami D, Kasuno K, Takahashi N, Hirano T, Yoshida H
  • Journal Title: Nephrology(Carlton) Volume: 16 Issue: 6 Pages: 558-566
  • DOI: 10.1111/j.1440-1797.2011.01454.x
  • Peer Reviewed
• [Journal Article] Glucocorticoid enhances hypoxia-and/or transforming growth factor-β-induced plasminogen activator inhibitor-1 production in human proximal renal tubular cells (2011)
  • Author(s): Kimura H, Li X, Torii K, Okada T, Kamiyama K, Mikami D, Kasuno K, Takahashi N, Yoshida H
  • Journal Title: Clin Exp Nephrol Volume: 15 Issue: 1 Pages: 34-40
  • DOI: 10.1007/s10157-010-0351-7
  • NAID: 10029004358
  • Peer Reviewed
• [Journal Article] 腎障害をきたす全身疾患-最新の進歩III.パラプロテイン血症 (2011)
  • Author(s): 高橋直生、木村秀撒、吉田治義、内木宏延
  • Journal Title: 日本内科学会雑誌 Volume: 100 Pages: 1282-1288
• [Journal Article] 腎臓病の扉をひらくキーワードレドックス制御因子チオレドキシン研究の臨床展開 (2011)
  • Author(s): 糟野健司、木村秀樹、高橋直生、三上大輔、横山由就、信川泰成、重見賢治、吉田治義
  • Journal Title: 臨床病理 Volume: 59 Pages: 189-195
• [Journal Article] Glucocorticoid enhances hypoxia- and/or transforming growth factor-ss-induced plasminogen activator inhibitor-1 production in human proximal renal tubular cells. (2011)
  • Author(s): Kimura H, Li X, Torii K, Okada T, Kamiyama K, Mikami D, Kasuno K, Takahashi N, Yoshida H.
  • Journal Title: Clin Exp Nephrol Volume: 15 Pages: 34-40
  • Peer Reviewed
• [Journal Article] Smaller LDL size as a possible risk factor for the prevalence of coronary artery diseases in hemodialysis patients-Associations of CETP and the hepatic lipase gene polymorphism with LDL size. (2011)
  • Author(s): Kimura H, Miyazaki R, Imura T, Masunaga S, Shimada A, Mikami D, Kasuno K, Takahashi N, Hirano T, Yoshida H.
  • Journal Title: Nephrology (Carlton) Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] 特集:広範囲血液・尿生化学検査,免疫学的検査(4)-その数値をどう読むか-Osteoprotegerin(OPG) (2010)
  • Author(s): 高橋直生, 木村秀樹, 糟野健司, 風間順一郎, 吉田治義
  • Journal Title: 日本臨床 Pages: 152-155
• [Journal Article] A specific serum IgA antibody discriminates pneumonia from colonization state in patients with Pseudomonas aeruginosa in sputum culture (2010)
  • Author(s): Masanori Yamashita, Yukio Hida, Hidehiro Sugimoto, Naoki Takahashi, Hideki Kimura, Yasuo Nakatomi, Haruyoshi Yoshida and Takeshi Ishizaki
  • Journal Title: J Microbiol Methods Volume: 82 Pages: 198-204
  • Peer Reviewed
• [Journal Article] Tubulointerstitial nephritis with IgM-positive plasmacytoid large lymphocyte infiltration in a patient with primary biliary cirrhosis and Sjoegren's syndrome (2010)
  • Author(s): Naoki Takahashi, Hideki Kimura, Yumiko Kawajiri, Daisuke Mikami, Chie Yamamoto, Kenji Kasuno, Naofumi Imai, Takeshi Kuroda, Shinichi Nishi, Makoto Yamamoto and Haruyoshi Yoshida
  • Journal Title: Clin Nephrol Volume: 74 Issue: 07 Pages: 74-80
  • DOI: 10.5414/cnp74074
  • Peer Reviewed
• [Journal Article] Tubulointerstitial nephritis with IgM-positive plasmacytoid large lymphocyte infiltration in a patient with primary biliary cirrhosis and Sjoegren's syndrome. (2010)
  • Author(s): Naoki Takahashi, Hideki Kimura, Yumiko Kawajiri, Daisuke Mikami, Chie Yamamoto, Kenji Kasuno, Naofumi Imai, Takeshi Kuroda, Shinichi Nishi, Makoto Yamamoto, Haruyoshi Yoshida.
  • Journal Title: Clin Nephrol Volume: 74 Pages: 74-80
  • Peer Reviewed
• [Journal Article] A specific serum IgA antibody discriminates pneumonia from colonization state in patients with Pseudomonas aeruginosa in sputum culture. (2010)
  • Author(s): Masanori Yamashita, Yukio Hida, Hidehiro Sugimoto, Naoki Takahashi, Hideki Kimura, Yasuo Nakatomi, Haruvoshi Yoshida, Takeshi Ishizaki.
  • Journal Title: J Microbiol Methods Volume: 198 Pages: 198-204
  • Peer Reviewed
• [Journal Article] Osteoprotegerin(OPG) (2010)
  • Author(s): 高橋直生、木村秀樹、糟野健司、吉田治義
  • Journal Title: 日本臨床増刊号 Volume: 68 Pages: 152-155
• [Journal Article] ネフローゼ症候群:診断と治療の進歩, III.二次性ネフローゼ症候群の病態3.その他の二次性ネフローゼ症候群 (2009)
  • Author(s): 高橋直生, 木村秀樹, 糟野健司, 三上大輔, 内木宏延, 吉田治義
  • Journal Title: 日本内科学会雑誌 Volume: 98 Pages: 75-81
• [Journal Article] Acute on chronic subdural hematoma as a rare complication in a microscopic polyangiitis patient receiving antithrombotic treatment (2009)
  • Author(s): Naoki Takahashi, Hideki Kimura, Ryuhei Kitai, Mamiko Sato, Makoto Yoneda, Chie Yamamoto, Daisuke Mikami, Masaru Kuriyama, Toshihiko Kubota, Hiroshi Itoh, and Haruyoshi Yoshida
  • Journal Title: Clin Nephrol Volume: 72 Pages: 211-215
  • Peer Reviewed
• [Journal Article] Dexamethasone enhances basal and TNF-alpha-stimulated production of PAI-1 via the glucocorticoid receptor regardless of 11beta-hydroxysteroid dehydrogenase 2 status in human proximalrenal tubular cells (2009)
  • Author(s): Kimura H, Li X, Torii K, Okada T, Kamiyama K, Mikami D, Takahashi N, Yoshida H
  • Journal Title: Nephrol Dial Transplan Volume: 24 Issue: 6 Pages: 1759-1765
  • DOI: 10.1093/ndt/gfn756
  • Peer Reviewed
• [Journal Article] Acute on chronic subdural hematoma as a rare complication in a microscopic polyangiitis patient receiving antithrombotic treatment (2009)
  • Author(s): Takahashi N, Kimura H, Kitai R, Sato M, Yoneda M, Yamamoto C, Mikami D, Kuriyama M, Kubota T, Itoh H, Yoshida H
  • Journal Title: Clin Nephrol 72 Pages: 211-215
  • Peer Reviewed
• [Journal Article] Dexamethasone enhances basal and TNF-α-stimulated production of PAI-1 via the glucocorticoid receptor regardless of 11β- hydroxysteroid dehydrogenase 2 status in human proximal renal tubular cells (2009)
  • Author(s): Kimura H, Li X, Torii K, Okada T, Kamiyama K, Mikami D, Takahashi N, Yoshida H
  • Journal Title: Nephrol Dial Transplant 24 Pages: 1759-1765
  • Peer Reviewed
• [Journal Article] 二次性ネフローゼ症候群の病態 3. その他の二次性ネフローゼ症候群 (2009)
  • Author(s): 高橋直生, 木村秀樹, 糟野健司, 三上大輔, 内木宏延, 吉田治義
  • Journal Title: 日本内科学会雑誌 98 Pages: 75-81
• [Presentation] Clinical and molecular-pathological effects of chronic hypoxia on diabetic nephropathy in db/db mice (2011)
  • Author(s): Naoki Takahashi
  • Organizer: 44th American Society of Nephrology annual meeting
  • Place of Presentation: Philadelphia
• [Presentation] 慢性低酸素環境におけるdb/dbマウスの糖尿病性腎症の進展機序の解析 (2011)
  • Author(s): 高橋直生
  • Organizer: 第54回日本腎臓学会
  • Place of Presentation: 横浜
• [Presentation] Overexpression of Human Liver-Fatty Acid-Binding Protein (L-FABP) Endogenously Enhances Peroxisome Proliferator-Activated Receptor (PPAR)-α Activity Possibly Via Inducing Hepatocyte Nuclear Factor (HNF)-4α in Immortalized Mouse Proximal Renal Tubular Cells (mProx) (2011)
  • Author(s): Hideki Kimura, Daisuke Mikami, Kazuko Kamiyama, Kenji Kasuno, Naoki Takahashi, Takeshi Sugaya, Haruyoshi Yoshida
  • Organizer: 44th American Society of Nephrology annual meeting
  • Place of Presentation: Philadelphia
• [Presentation] Urinary Thioredoxin 1 Is an Oxidative Stress-Specific Biomarker of Acute Kidney Injury (2011)
  • Author(s): Kenji Kasuno, Eri Muso, Hideki Kimura, Naoki Takahashi, Yasunari Nobukawa, Kiyoshi Mori, Haruyoshi Yoshida
  • Organizer: 44th American Society of Nephrology annual meeting
  • Place of Presentation: Philadelphia
• [Presentation] レドックス制御蛋白「尿中チオレドキシン」の酸化ストレス特異的急性腎障害(AKI)マーカーとしての有用性 (2011)
  • Author(s): 糟野健司, 木村秀樹、高橋直生, 三上大輔, 横山由就、武曾恵里、信川泰成, 重見研司、吉田治義
  • Organizer: 第54回日本腎臓学会
  • Place of Presentation: 横浜
• [Presentation] 血液透析(HD)患者の虚血性心疾患(CAD)の危険因子としての低比重リポ蛋白(LDL)サイズの減少 (2011)
  • Author(s): 木村秀樹,三上大輔, 嶋田章弘、宮崎良一、糟野健司, 高橋直生, 吉田治義、平野勉
  • Organizer: 第54回日本腎臓学会
  • Place of Presentation: 横浜
• [Presentation] 血液透析患者の鉄欠乏性貧血における網赤血球ヘモグロビン等量の有用性 (2011)
  • Author(s): 横山由就, 三上大輔, 糟野健司, 高橋直生, 木村秀樹, 山本智恵, 宮城恭子, 宮崎良一, 吉田治義
  • Organizer: 第56回日本透析医学会
  • Place of Presentation: 横浜
• [Presentation] 急性腎障害(AKI)の病因別診断に尿中チオレドキシンが有用である (2011)
  • Author(s): 糟野健司、木村秀樹、高橋直生, 三上大輔, 横山由就, 村瀬詠廉, 横井靖二, 信川泰成, 吉田治義
  • Organizer: 第58回日本臨床検査医学会
  • Place of Presentation: 岡山
• [Presentation] 慢性低酸素環境におけるdb/dbマウスの糖尿病性糸球体硬化の進展 (2010)
  • Author(s): 高橋直生, 木村秀樹, 糟野健司, 三上大輔, 井村敏雄, 杉本英弘, 吉田治義
  • Organizer: 日本腎臓学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-06-17
• [Presentation] Acceleration of Diabetic Glomerulopathy in db/db Mice in Chronic Hypoxia (2010)
  • Author(s): Naoki Takahashi
  • Organizer: 43th American Society of Nephrology annual meeting
  • Place of Presentation: Denver
• [Presentation] 慢性低酸素環境におけるdb/dbマウスの糖尿病性糸球体硬化の進展 (2010)
  • Author(s): 高橋直生
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: 神戸
• [Presentation] 高糖、炎症、低酸素状態下の近位尿細管細胞におけるVEGF-A、C発現動態の解析ステロイド作用の検討も含めて (2010)
  • Author(s): 木村秀樹, 杉本英弘, 上山和子, 三上大輔, 糟野健司, 高橋直生, 吉田治義
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: 神戸
• [Presentation] 血液透析患者における低比重リポ蛋白サイズの独立規定因子の解析 (2010)
  • Author(s): 木村秀樹, 宮崎良一, 井村敏雄, 嶋田章弘, 増永慎也, 三上大輔, 糟野健司, 高橋直生, 平野勉, 吉田治義
  • Organizer: 第57回日本臨床検査医学会
  • Place of Presentation: 東京
• [Presentation] 腎不全患者を対象とした活性化血小板の測定および血小板凝集能検査との比較検討 (2010)
  • Author(s): 岡田敏春, 高橋直生, 鳥居国雄, 山野智子, 関口清美, 木村秀樹, 吉田治義
  • Organizer: 第57回日本臨床検査医学会
  • Place of Presentation: 東京
• [Presentation] 低酸素下のヒト近位尿細管細胞において、グルココルチコイドはPAI-1の産生を増強しVEGFの産生を抑制する (2010)
  • Author(s): 鳥居国雄, 木村秀樹, 上山和子, 杉本英弘, 岡田敏春, 山野智子, 関口清美, 三上大輔, 糟野健司, 高橋直生, 吉田治義
  • Organizer: 第57回日本臨床検査医学会
  • Place of Presentation: 東京
• [Presentation] Acceleration of Diabetic Glomerulopathy in db/db Mice in Chronic Hypoxia. (2010)
  • Author(s): Naoki Takahashi, Hideki Kimura, Kazuko Kamiyama, Tomomi Kurose, Hidehiro Sugimoto, Toshio Imura, Dai suke Mikami, Kenji Kasuno, Haruyo shi Yoshida.
  • Organizer: 43th American Society of Nephrology annual meeting
  • Place of Presentation: Denver
• [Presentation] LDL Size Reduction as a Possible Risk Factor for the Prevalence of Coronary Artery Diseases in Hemodialysis Patients. (2010)
  • Author(s): Hideki Kimura, Ryoichi Miyazaki, Shinya Masunaga, Akihiro Shimada, Toshio Imura, Daisuke Mikami, Kenji Kasuno, Naoki Takahashi, Tsutomu Hirano, Haruyoshi Yoshida.
  • Organizer: 43th American Society of Nephrology annual meeting
  • Place of Presentation: Denver
• [Presentation] Discrete Regulation of VEGF-A and -C in Human Proximal Renal Tubular Cells under Hypoxic and/or Inflammatory Conditions-Inhibitory Effects of Glucocorticoids on Basal and Inducible VEGF Expression. (2010)
  • Author(s): Hideki Kimura, Hidehiro Sugimoto, Kazuko Kamiyama, Daisuke Mikami, Kenji Kasuno, Naoki Takahashi, Haruyoshi Yoshida.
  • Organizer: 43th American Society of Nephrology annual meeting
  • Place of Presentation: Denver
• [Presentation] Urinary Thioredoxin Is a Quick and Predictive Biomarker of Acute Kidney Injury. (2010)
  • Author(s): Kenji Kasuno, Eri Muso, Daisuke Mikami, Naoki Takahashi, Hideki Kimura, Tomomi Kurose, Yasunari Nobukawa, Haruyoshi Yoshida.
  • Organizer: 43th American Society of Nephrology annual meeting
  • Place of Presentation: Denver
• [Remarks]
  • URL: http://jinnai.labos.ac/
• [Remarks]
  • URL: http://jinnai.med.lab.u-fukui.ac.jp/ja/document/
• [Remarks]
  • URL: http://www1.med.u-fukui.ac.jp/JINNAI/index_files/gyoseki.htm