Utility of separation technique of live cancer stem cells with using an energy transfer fluorescence probes
Project/Area Number |
21590622
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Laboratory medicine
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Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
OHNISHI Kazunori 浜松医科大学, 医学部附属病院, 教授 (80252170)
竹下 香 (新庄 香) 浜松医科大学, 保健管理センター, 特任助教 (30397393)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | エネルギー移行 / cell sorter / mRNA / 白血病 / 幹細胞 / FRET法 / KRAS / 癌細胞 / 白血病細胞 / bcr-abl / Kras / 肝細胞 |
Research Abstract |
QUAL/FRET method is effective to determine the cells in which have specific mRNA. In this study, we examined the efficacy on insertion of probes using fluorescein labeled dextran firstly. Relatively high molecular ones did not enter into nucleus and distributed in cytoplasm. On the other hand, the efficacy of the insertion was studied in cell lines and peripheral blood cells. The efficacy was not sufficient in adherent cells and lymphocytes. It should be improved by changing molecular size of probe and conjugated fluorescence.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] for the Japan Adult Leukemia Study Group. Phase I trial of gemtuzumab ozogamicin in intensive combination chemotherapy for relapsed or refractory adult acute myeloid leukemia(AML): Japan Adult Leukemia Study Group(JALSG)-AML206 study2011
Author(s)
Usui N, Takeshita A, Nakaseko C, Dobashi N, Fujita H, Kiyoi H, Kobayashi Y, Sakura T, Yahagi Y, Shigeno K, Ohwada C, Miyazaki Y, Ohtake S, Miyawaki S, Naoe T, Ohnishi K
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Journal Title
Cancer Sci
Volume: 102
Pages: 1358-1365
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[Journal Article] CMC-544(inotuzumab ozogamicin) shows less effect on multidrug resistant cells : analyses in cell lines and cells from patients with B-cell chronic lymphocytic leukaemia and lymphoma2009
Author(s)
Takeshita A, Shinjo K, Yamakage N, Ono T, Hirano I, Matsui H, Shigeno K, Nakamura S, Tobita T, Maekawa M, Ohnishi K, Sugimoto Y, Kiyoi H, Naoe T, Ohno R
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Journal Title
Br J Haematol
Volume: 146(1)
Pages: 34-43
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[Journal Article] Effective Blood Utilization via System for Massive Blood Transfusion, including Cardiovascular Operation in Local Areas2009
Author(s)
Takeshita A, Asai T, Murakami M, Fujihara H, Ishizuka T, Nakai S, Yamada C, Suzumura T, Uchiyama Y, Maekawa M, Shigeno K, Washiyama N, Yamashita K, Unno N, Shinjo K
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Journal Title
Jpn J Transfusion Cell Therapy
Volume: 55(1)
Pages: 63-7
NAID
Related Report
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[Journal Article] CMC-544(inotuzumab ozogamicin), an anti-CD22immuno-conjugate of calicheamicin, alters the levels of target molecules of malignant B-cells2009
Author(s)
Takeshita A, Yamakage N, Shinjo K, Ono T, Hirano I, Nakamura S, Shigeno K, Tobita T, Maekawa M, Kiyoi H, Naoe T, Ohnishi K, Sugimoto Y, Ohno R
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Journal Title
Leukemia
Volume: 23(7)
Pages: 1329-36
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[Journal Article] Phase I/II study of humanized anti-CD33antibody conjugated with calicheamicin, gemtuzumab ozogamicin, in relapsed or refractory acute myeloid leukemia : final results of Japanese multicenter cooperative study2009
Author(s)
Kobayashi Y, Tobinai K, Takeshita A, Naito K, Asai O, Dobashi N, Furusawa S, Saito K, Mitani K, Morishima Y, Ogura M, Yoshiba F, Hotta T, Bessho M, Matsuda S, Takeuchi J, Miyawaki S, Naoe T, Usui N, Ohno R
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Journal Title
Int J Hematol
Volume: 89(4)
Pages: 460-9
NAID
Related Report
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[Presentation] Efficacy of gemtuzumab ozogamicin(GO) on relapsed/refractory acute promyelocytic leukemia(APL)2011
Author(s)
Takeshita A, Ono T, Kojima Y, Kyo T, Asou N, Suzushima H, Yagasaki H, Maeda T, Okada M, Nakaseko C, Kanbayashi H, Yamaguchi M, Kurosawa M, Tsuboi K, Yjiri T, Nanya Y, UikeN, Akiyama N, Fukuda T, Karimata K, Okumura H, Eto T, Izumiyama K, Watanabe A, Aotsuka N, Ito K, Ozaki K, Naoe T
Organizer
53rd Annual Meeting of American Society of Hematology San Diego
Place of Presentation
CA, USA
Year and Date
2011-12-10
Related Report
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[Presentation] Differences in allo-immunity to erythrocytes between Korea and Japan. Cooperative international study of alloimmunity to antigen diversity in Asian population2011
Author(s)
Fujihara H, Takeshita A, Kim DW, Han KS, Kwon SY, Kwon SW, Suh JS, Watanabe H, Uchikawa M, Kino S, Nollet KE, Ohto H
Organizer
53rd Annual Meeting of American Society of Hematology San Diego
Place of Presentation
CA, USA
Year and Date
2011-12-10
Related Report
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[Presentation] Clinical features and prognostic impact of CD56expression in acute promyelocytic leukemia : Long term follow up data from the Japan Adult Leukemia Study Group(JALSG) APL972011
Author(s)
Ono T, Takeshita A, Kishimoto Y, Kiyoi H, Okada M, Yamauchi T, Tsuzuki M, Horikawa K, Matsuda M, Shinagawa K, Monma F, Ohtake S, Nakaseko C, Takahashi M, Yagasaki F, Kimura Y, Fujita H, Iwanaga M, Asou N, Ohnishi K, Naoe T
Organizer
53rd Annual Meeting of American Society of Hematology San Diego
Place of Presentation
CA, USA
Year and Date
2011-12-10
Related Report
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[Presentation] Role of hematopoietic stem cell transplantation as salvage treatment of acute promyelocytic leukemia initially treated with all-trans retinoic acid : a retrospective analysis of the Japan Adult Leukemia Study Group(JALSG) APL97Study2011
Author(s)
Fujita H, Asou N, Iwanaga M, Hyo R, Nomura S, Kiyoi H, Okada M, Tsuzuki M, Matsuda M, Yamauchi T, Ohtake S, Izumi T, Nakaseko C, Mitani K, Shinagawa K, Takeshita A, Miyazaki Y, Ohnishi K, Miyawaki S, Naoe T
Organizer
53rd Annual Meeting of American Society of Hematology San Diego
Place of Presentation
CA, USA
Year and Date
2011-12-10
Related Report
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[Presentation] Impact of additional chromosomal abnormalities(ACAs) in patients of acute promyelocytic leukemia10years follow up results of the Japan Adult Leukemia Study Group APL97study2010
Author(s)
Ono T, Takeshita A, Kishimoto Y, Kiyoi H, Okada M, Kawai Y, Tsuzuki M, Horikawa K, Matsuda M, Shinagawa K, Monma F, Ohtake S, Nakaseko C, Takahashi M, Yagasaki F, Kimura Y, Iwanaga M, Asou N, Naoe T, Ohno R
Organizer
15th Congress of the European Hematology Association
Place of Presentation
Barcelona, Spain
Related Report
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[Presentation] Clinical Features and Outcomes of Elderly Patients with Acute Promyelocytic Leukemia(APL)-the Japan Adult Leukemia Study Group APL97Study2010
Author(s)
Ono T, Takeshita A, Kishimoto Y, Kiyoi H, Okada M, Kawai Y, Tsuzuki M, Horikawa K, Matsuda M, Shinagawa K, Monma F, Ohtake S, Nakaseko C, Takahashi M, Yagasaki F, Kimura Y, Iwanaga M, Asou N, Naoe T, Ohno R
Organizer
52nd annual meeting of American Society of Hematology
Place of Presentation
Orland, FL, USA
Related Report
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[Presentation] Erythrocyte alloimmunization analyzed by gender and transfusion status2010
Author(s)
Takeshita A, Watanabe H, Fujihara H, Oshida M, Yurugi K, Tomoda Y, Uchikawa M, Kino S, Nollet KE, Ohto H
Organizer
52nd American Society
Place of Presentation
Orland, FL, USA
Related Report
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[Presentation] Collaborative Study Group of Allo-immunity to Antigen Diversity in Asian Populations. Differences in allo-immunity to erythrocytes in Asian countries ; Cooperative study of allo-immunity to antigen diversity in Asian populations2009
Author(s)
Watanabe H, Takeshita A, Kim DW, Han KS, Kwon SY, Suh JS, Natalie CPH, Nadarajan VS, Permpikul P, Nuranissa Bte Yacob Marican NBY, Uchikawa M, Kino S, Ohto H
Organizer
51st Annual Meeting of the American Society of Hematology
Place of Presentation
New Orleans, LA, USA
Related Report
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[Presentation] Collaborative Study Group of Allo-immunity to Antigen Diversity in Asian Populations. Allo-immunity to erythrocytes in relation to blood transfusion and pregnancy ; Cooperative study of allo-immunity to antigen diversity in Asian populations2009
Author(s)
Takeshita A, Watanabe H, Kim DW, Han KS, Kwon SY, Suh JS, Natalie CPH, Nadarajan VS, Permpikul P, Nuranissa Bte Yacob Marican NBY, Uchikawa M, Kino S, Ohto H
Organizer
51st Annual Meeting of the American Society of Hematology
Place of Presentation
Orleans, LA, USA
Related Report
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[Presentation] CMC-544, an anti-CD22immunoconjugate of calicheamicin, decreases the level of compliment inhibitory factors on malignant B-cell lymphoma cells2009
Author(s)
Takeshita A, Yamakage N, Shinjo K, Hirano I, Ono T, Fujihara H, Watanabe H, Iino K, Hashimoto D, Maekawa M, Ohnishi K, Ohno R
Organizer
14nd Congress of European Society of Hematology
Place of Presentation
Berlin, Germany
Related Report
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[Presentation] A Phase I/II Trial of Gemtuzumab Ozogamicin in Combination with Cytarabine and Anthracyclines(Daunorubicin or Idarubicin) for CD33-Positive Refractory or Relapsed AML Patients Younger Than65Years. Results of Phase I Parts of the JALSG-AML206Study2009
Author(s)
Usui N, Takeshita A, Nakaseko C, Dobashi N, Fujita H, Kiyoi H, Kobayashi Y, Fujisawa S, Yagasaki F, Nishii K, Kiguchi T, Miyazaki Y, Ohtake S, Miyawaki S, Ohno R, Naoe T, Ohnishi K
Organizer
51st Annual Meeting of the American Society of Hematology
Place of Presentation
New Orleans, LA, USA
Related Report
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[Presentation] Collaborative Study Group of Allo-immunity to Antigen Diversity in Asian Populations : Differences in allo-immunity to erythrocytes in Asian countries ; Cooperative study of allo-immunity to antigen diversity in Asian populations2009
Author(s)
Watanabe H, Takeshita A, Kim DW, Han KS, Kwon SY, Suh JS, Natalie CPH, Nadarajan VS, Permpikul P, Nuranissa Bte Yacob Marican NBY, Uchikawa M, Kino S, Ohto H
Organizer
51st Annual Meeting of the American Society of Hematology
Place of Presentation
New Orleans, LA, USA
Related Report
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