| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Research Abstract |
Purpose : Although the fecal occult blood test is performed daily as a primary screening test, sensitivity is low, and false positive reactions may occur when hemorrhoids are present.
Fecal DNA analysis appears to be a valid alternative method providing more advanced and reliable detection of CRC. Thus, we evaluated whether the DNA test could improve diagnostic performance for CRC detection.
Methods : DNA was isolated from stools of patients with CRC and Twist1 methylation level was evaluated using pyrosequencing.
Results : We found TWIST1 methylation occurred in feces with a sensitivity of 18% for detection of CRC.
Conclusions : TWIS1 methylation assay may be useful for the detection of CRC.
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