| Project/Area Number |
21590637
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Junji 自治医科大学, 医学部, 研究員 (90536301)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 臨床化学 / アミロイドーシス / 血清アミロイドA / 表面プラズモン共鳴 / 自己抗体 |
|---|
| Research Abstract |
The purpose of this study was the development of the method using SPR analysis(Biacore), which could detect low affinity antibodies. As a model, anti-amyloid antibodies, which might appear in AA-amyloidosis, the major complication in rheumatoid arthritis, were targeted. In the process of investigation, we succeeded in establishing monoclonal anti-SAA, either anti-mouse or anti-human, antibodies. Especially, an antibody specific to AA carboxyl-terminus generated from SAA degradation during amyloidogenic process must be useful for the further investigation in fundamental and clinical aspects in AA-amyloidosis. For antibody detection using Biacore, a sensor chip immobilized with anti-AA antibodies was reacted in order with extracted amyloid tissues, patient serum, and anti-human immunoglobulin. The system worked when anti-SAA antibodies were used instead of patient serum. Thus, the system itself is able to be applied for detection of other autoantibodies. However, the trails using patient sera failed to detect the significant antibody activity. To amplify the sensitivity may be the next problem to improve.
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