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Studies on combined effects of low-dose cadmium and environmental chemicals

Research Project

Project/Area Number 21590651
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionUniversity of Toyama

Principal Investigator

INADERA Hidekuni  富山大学, 大学院・医学薬学研究部(医学), 教授 (10301144)

Research Collaborator CUI Zheng-guo  富山大学, 大学院・医学薬学研究部(医学), 助教 (90572115)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords環境保健 / カドミウム / アポトーシス / 化学物質
Research Abstract

Cadmium(Cd) is a potential environmental hazard that induces disorders in various organs. In this study, we examined the molecular mechanisms involved in the adaptive response to Cd-induced apoptosis in human myelomonocytic lymphoma U937 cells. When U937 cells were treated with 50μM cadmium chloride(CdCl2) for 12 hours, significant apoptosis occurred. This was associated with an increase in intracellular reactive oxygen species(ROS), sustained phosphorylation of JNK, activation of caspase-3, a decrease in Mcl-1(anti-apoptotic Bcl-2 proteins), and increases in Bim, Noxa and tBid(a pro-apoptotic protein under the Bcl-2 family). No apoptosis occurred when the cells were treated with 1μM CdCl2 for 72 hours. However, pretreatment with low-dose CdCl2 dramatically altered the sensitivity of the cells to 50μM CdCl2 with inhibition of apoptosis. Concomitantly, there were significant decreases in the generation of intracellular ROS and the activation of JNK. Pretreatment with 1μM CdCl2 also attenuated the decrease in Mcl-1 and the increases in Bim, Noxa and tBid induced by 50μM CdCl2. The inhibition of apoptosis following exposure to low-dose Cd suggests a potential mechanism that may help explain why people exposed to low doses of Cd for a long time have an increased risk of developing certain cancers.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (19 results)

All 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (6 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Molecular mechanisms involved in the adaptive response to cadmium-induced apoptosis in human myelomonocytic lymphoma U937 cells2011

    • Author(s)
      Cui Z-G, Ogawa R, Piao J-L, Hamazaki K, Feril L. B, Shimomura A, Kondo T, and Inadera H
    • Journal Title

      Toxicol. in vitro

      Volume: 25 Pages: 1687-1693

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Toxicity monitoring with primary cultured hepatocytes underestimates the acetaminophen-induced inflammatory responses of the mouse liver2011

    • Author(s)
      Tachibana S, Shimomura A, and Inadera H
    • Journal Title

      Tohoku J. Exp. Med

      Volume: 225 Pages: 263-272

    • NAID

      10030196428

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Docosahexaenoic acid is an independent predictor of all-cause mortality in hemodialysis patients2011

    • Author(s)
      Hamazaki K, Terashima Y, Itomura M, Sawazaki S, Inagaki H, Kuroda M, Tomita S, Hirata H, Inadera H, and Hamazaki T.
    • Journal Title

      Am. J. Nephrol

      Volume: 33 Pages: 105-110

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms involved in the adaptive response to cadmium-induced apoptosis in human myelomonocytic lymphoma U937 cells2011

    • Author(s)
      Cui ZG, Inadera H, et al
    • Journal Title

      Toxicology in vitro

      Volume: 25 Pages: 1687-1693

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hyperglycemia perturbs biochemical networks in human trophoblast BeWo cells2010

    • Author(s)
      nadera H, Tachibana S, Takasaki I, Tatematsu M, and Shimomura A.
    • Journal Title

      Endocr. J

      Volume: 57 Pages: 567-577

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Carbon tetrachloride affects inflammation-related biochemical networks in the mouse liver as identified by a customized cDNA microarray system.2010

    • Author(s)
      Inadera H, Tachibana S, Suzuki A, and Shimomura A.
    • Journal Title

      Environ Health Prev. Med

      Volume: 15 Pages: 105-114

    • NAID

      10026960129

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hyperglycemia perturbs biochemical networks in human trophoblast BeWo cells.2010

    • Author(s)
      Inadera H., Tachibana S., Takasaki I., Tatematsu M., Shimomura A
    • Journal Title

      Endocr.J.

      Volume: 57 Pages: 567-577

    • NAID

      10029585441

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Carbon tetrachloride affects inflammation-related biochemical networks in the mouse liver as identified by a customized cDNA microarray system.2010

    • Author(s)
      Inadera H, et al.
    • Journal Title

      Environ.Health Prev.Med. 177

      Pages: 20-30

    • NAID

      10026960129

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Wnt-1 inducible signaling pathway protein-2(WISP-2/CCN5), a downstream protein of Wnt signaling, on adipocyte differentiation. Biochem2009

    • Author(s)
      Inadera H, Shimomura A, and Tachibana S
    • Journal Title

      Biophys. Res. Commun

      Volume: 379 Pages: 969-974

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Wnt-1 inducible signaling pathway protein-2(WISP-2/CCN5), a downstream protein of Wnt signaling, on adipocyte differentiation.2009

    • Author(s)
      Inadera H, et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 379

      Pages: 969-974

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] ドコサヘキサエン酸による温熱誘発アポトーシスの増強とその分子メカニズムの検討2012

    • Author(s)
      崔正国,近藤隆,稲寺秀邦
    • Organizer
      第82回日本衛生学会総会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-25
    • Related Report
      2011 Final Research Report
  • [Presentation] トランスクリプトーム解析を用いた高血糖の胎盤絨毛細胞分化に及ぼす影響の検討2012

    • Author(s)
      稲寺秀邦,崔正国,橘信二郎
    • Organizer
      第82回日本衛生学会総会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 低濃度カドミウム(Cd)前処理による高濃度Cd誘発アポトーシス抵抗性の獲得機構の検討2011

    • Author(s)
      崔正国,小川良平,浜崎景,寺西秀豊,近藤隆,稲寺秀邦
    • Organizer
      第81回日本衛生学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2011-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞内活性酸素生成の修飾による温熱誘発アポトーシスの増強2010

    • Author(s)
      崔正国,稲寺秀邦,寺西秀豊,浜崎景,近藤隆
    • Organizer
      第80回日本衛生学会学術集会
    • Place of Presentation
      仙台
    • Year and Date
      2010-05-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 有害化学物質の生体影響評価のためのDNAマイクロアレイの構築2010

    • Author(s)
      稲寺秀邦,橘信二郎,崔正国,下村明子
    • Organizer
      第80回日本衛生学会学術集会
    • Place of Presentation
      仙台
    • Year and Date
      2010-05-10
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 四塩化炭素長期曝露による抗酸化酵素の誘導2009

    • Author(s)
      橘信二郎、内田満夫、常山幸一、稲寺秀邦
    • Organizer
      第79回日本衛生学会
    • Place of Presentation
      北里大学(東京)
    • Year and Date
      2009-03-30
    • Related Report
      2009 Annual Research Report
  • [Book] 環境エストロゲン「分子予防環境医学」2010

    • Author(s)
      稲寺秀邦
    • Publisher
      本の泉社(東京)
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.med.u-toyama.ac.jp/pubhlth/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.med.u-toyama.ac.jp/pubhlth/index.html

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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